A5012464432- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Weed Control and Herbicide Applications
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Mycorrhizal Fungi and Plant Interactions
- Genetic Mapping and Diversity in Plants and Animals
- Toxin Mechanisms and Immunotoxins
- Polyamine Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Herbal Medicine Research Studies
- GABA and Rice Research
- Plant Molecular Biology Research
- Pharmacological Effects of Natural Compounds
South China Agricultural University
2016-2025
Xinxiang Medical University
2024
First Affiliated Hospital of Xinxiang Medical University
2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2015-2023
Shantou University
2021
Universiti Malaysia Terengganu
2021
Shell (Malaysia)
2021
South Central Minzu University
2010
State Ethnic Affairs Commission
2010
Huazhong Agricultural University
1999
• The rice–rice blast pathosystem is of great interest, not only because the damaging potential rice to crop, but also both pathogen and its host are experimentally amenable. resistance gene Pik, which one five classical alleles located at Pik locus on long arm chromosome 11, confers high stable many Chinese isolates. isolation functional characterization were performed in present study through genetic genomic approaches. A combination Pik-1 Pik-2 required for expression resistance. Both...
Abstract The resistance (R) gene Pi37, present in the rice cultivar St. No. 1, was isolated by an silico map-based cloning procedure. equivalent genetic region Nipponbare contains four nucleotide binding site–leucine-rich repeat (NBS–LRR) type loci. These candidates for Pi37 (Pi37-1, -2, -3, and -4) were amplified separately from 1 via long-range PCR, cloned into a binary vector. Each construct individually transformed highly blast susceptible Q1063. subsequent complementation analysis...
The indica rice variety Kasalath carries Pi36, a gene that determines resistance to Chinese isolates of blast and has been located 17-kb interval on chromosome 8. genomic sequence the reference japonica Nipponbare was used for an in silico prediction (R) content hence identification candidate gene(s) Pi36. Three such sequences, which all had both nucleotide-binding site leucine-rich repeat motif, were present. three genes amplified from DNA number varieties by long-range PCR, resulting...
Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair NBS-LRR genes, Pikh-1 and Pikh-2. While appears to be constitutively transcribed, transcript abundance Pikh-2 responds challenge. The CC (coiled coil) domain interacts directly with both AvrPik-h Transient expression assays demonstrated mediates initiation host defence response. Nucleocytoplasmic partitioning is required for their...
Abstract The fall armyworm (FAW), Spodoptera frugiperda , is a destructive pest native to America and has recently become an invasive insect in China. Because of its rapid spread great risks China, understanding FAW genetic background pesticide resistance urgent essential develop effective management strategies. Here, we assembled chromosome-level genome male (SFynMstLFR) compared re-sequencing results the populations from America, Africa, Strain identification 163 individuals collected...
A stepwise mutation that occurred in both pathogens and their respective hosts has played a seminal role the co-evolutionary arms race evolution diverse pathosystems. The process driven by rice blast AvrPik Pik alleles was investigated through population genetic evolutionary approaches. diversity of non-signal domain higher than its signal peptide domain. Positive selection for particular northeastern region China stronger south. perfect relationship between functional lineages...
To control the development of resistance to conventional insecticides acting as γ-aminobutyric acid (GABA) receptor antagonists (e.g., fipronil), new GABAergic 5,5-disubstituted 4,5-dihydropyrazolo[1,5-a]quinazolines were designed via a scaffold-hopping strategy and synthesized with facile method. Among 50 target compounds obtained, 5a, 5b, 7a, 7g showed excellent insecticidal activities against susceptible strain Plutella xylostella (LC50 values ranging from 1.03 1.44 μg/mL), which superior...
Four new limonoid-type nortriterpenoids, 1-detigloyl-1-O-methacryloylsalannin (1), 28-deoxo-2,3-dihydronimbolide (2), 12-acetoxy-3-O-acetyl-7-O-tigloylvilasinin (3) and 12-acetoxy-3-O-acetyl-7-O-methacryloylvilasinin (4), along with five known ones, were isolated from seeds of Azadirachta indica A. Juss. Their structures elucidated by various spectroscopic methods, including UV, IR, MS, NMR, X-ray crystallography, quantum chemical calculation, as well comparison their data those reported. In...
Phloem-mobile insecticides are efficient for piercing and sucking insect control. Introduction of sugar or amino acid groups to the parent compound can improve phloem mobility insecticides, so a glycinergic–fipronil conjugate (GlyF), 2-(3-(3-cyano-1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((trifluoromethyl)sulfinyl)-1H-pyrazole-5-yl)ureido) acetic acid, was designed synthesized. Although "Kleier model" predicted that this is not mobile, GlyF be continually detected during 5 h collection...
Characterization and genetic engineering of plant transporters involved in the pesticide uptake translocation facilitate relocation to tissue where pests feed, thus improving bioavailability agrichemicals. We aimed identify thiamethoxam (THX) rice modify their expression for better brown planthopper (BPH) control with less application. A yeast library expressing 1385 was screened, leading identification an amino acid transporter-like (ATL) gene, namely OsATL15, which facilitates THX both...
Pik-p is carried by cv. K60, which one of the Japanese differentials widely used in both Japan and China since 1980s. Its utility specificity was evaluated with a total 612 isolates Magnaporthe oryzae collected from various regions combination 16 main resistance genes being breeding programs. an independently dominantly acting gene Pik cluster, conditions differential reactions against many contains higher Guangdong, Jiangsu, Sichuan provinces, China, indicating that this could be still...
Low-altitude mobility, exemplified by unmanned aerial vehicles (UAVs), has introduced transformative advancements across various domains, like transportation, logistics, and agriculture. Leveraging flexible perspectives rapid maneuverability, UAVs extend traditional systems' perception action capabilities, garnering widespread attention from academia industry. However, current UAV operations primarily depend on human control, with only limited autonomy in simple scenarios, lack the...
Abstract Controlling weeds before their emergence is crucial for minimizing impacts on crop yield and quality. Bipyridyl herbicides (BPyHs), a class of highly effective broad-spectrum herbicides, cannot be used as pre-emergence because they can absorbed inactivated by negatively charged soil after application. Here, we design fabricate an adsorbed-but-active supramolecular herbicide consisting cationic polyacrylamide bipyridyl (CPAM-BPyHs). CPAM positively polymer. It preferentially bind to...
The elite rice cultivar Yuejingsimiao 2 (YJ2) is characterized by a high level of grain quality and yield, resistance against Magnaporthe oryzae. YJ2 showed 100% to four fungal populations collected from Guangdong, Sichuan, Liaoning, Heilongjiang Provinces, which higher frequency than that shown the well-known (R) gene donor cultivars such as Sanhuangzhan 28zhan. Segregation analysis for with F(2) F(4) indicated was controlled multiple genes are dominant or recessive. putative R were roughly...
Creating novel pesticides with phloem mobility is essential for controlling insects in vascular tissue and root, conjugating existing amino acid an effective approach. In order to obtain a highly phloem-mobile candidate efficient pesticides, electro-neutral l-glutamine-fipronil conjugate (l-GlnF) retaining α-amino function was designed synthesized fit the substrate specificity of transporter. Cotyledon uptake loading tests Ricinus communis have verified that l-GlnF mobile, its higher than...
The phenylpyrazole insecticide fipronil blocks resistance to dieldrin (RDL) γ-aminobutyric acid (GABA) receptors in insects, thereby impairing inhibitory neurotransmission. Some insect species, such as the diamondback moth (Plutella xylostella), possess more than one Rdl gene. involvement of multiple Rdls toxicity and remains largely unknown. In this study, we investigated roles two genes, PxRdl1 PxRdl2, P. xylostella action. Xenopus oocytes, PxRDL2 were 40 times less sensitive PxRDL1. also...
In our previous study, a series of novel pyrazoloquinazolines were synthesized. Pyrazoloquinazoline 5a showed high insecticidal activity against the diamondback moth (Plutella xylostella) and no cross-resistance to fipronil. Patch clamp electrophysiology performed on P. xylostella pupae brains two-electrode voltage Xenopus Laevis oocytes indicated that might act ionotropic γ-aminobutyric acid (GABA) receptor (GABAR) glutamate-gated chloride channel (GluCl). Moreover, 5a's potency PxGluCl was...