- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Rice Cultivation and Yield Improvement
- Genomics and Phylogenetic Studies
- Wheat and Barley Genetics and Pathology
- Plant responses to water stress
- GABA and Rice Research
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Genetics and Plant Breeding
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Plant Gene Expression Analysis
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Seed Germination and Physiology
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Plant Virus Research Studies
Sichuan Agricultural University
2024
National Agriculture and Food Research Organization
2017-2023
Institute of Crop Science
2017-2023
Changzhi University
2019
Shanxi Normal University
2019
Institute of Agrobiological Sciences
2009-2018
Agrogen (Czechia)
2013-2015
Tokyo Medical and Dental University
2011
Center for Genomic Science
2011
RIKEN BioResource Research Center
2011
Abstract Inflorescence architecture is a key agronomical factor determining grain yield, and thus has been major target of cereal crop domestication. Transition from spread panicle typical ancestral wild rice ( Oryza rufipogon Griff.) to the compact present cultivars O. sativa L.) was crucial event in Here we show that controlled by dominant gene, Os LG1, previously reported SBP-domain transcription controls ligule development. Association analysis indicates single-nucleotide polymorphism-6...
Summary We constructed physical maps of rice chromosomes 1, 2, and 6–9 with P1‐derived artificial chromosome (PAC) bacterial (BAC) clones. These maps, only 20 gaps, cover more than 97% the predicted length six chromosomes. submitted a total 193 Mbp non‐overlapping sequences to public databases. analyzed DNA 1316 genetic markers centromere‐specific repeats facilitate characterization chromosomal recombination frequency genomic composition structure centromeric regions. found marked changes in...
In rice (Oryza sativa L.), there is a diversity in flowering time that strictly genetically regulated. Some indica cultivars show extremely late under long-day conditions, but little known about the gene(s) involved. Here, we demonstrate functional defects florigen gene RFT1 are main cause of an cultivar, Nona Bokra. Mapping and complementation studies revealed sequence polymorphisms regulatory coding regions likely to conditions. We detected promoter region lead reduced expression levels...
Wheat yellow mosaic virus (WYMV) is a pathogen transmitted into its host's roots by the soil-borne vector Polymyxa graminis. Ym1 and Ym2 genes protect host from significant yield losses caused virus, but mechanistic basis of these resistance remains poorly understood. Here, it has been shown that act within root either hindering initial movement WYMV and/or suppressing viral multiplication. A mechanical inoculation experiment on leaf revealed presence reduced infection incidence, rather than...
Abstract Background Microarray technology is limited to monitoring the expression of previously annotated genes that have corresponding probes on array. Computationally not fully been validated, because ESTs and full-length cDNAs cannot cover entire transcribed regions. Here, mRNA-Seq (an Illumina cDNA sequencing application) was used monitor whole mRNAs salinity stress-treated rice tissues. Results Thirty-six-base-pair reads from were mapped genomic sequence: 72.0% 75.2% uniquely genome,...
Having a deep genetic structure evolved during its domestication and adaptation, the Asian cultivated rice (Oryza sativa) displays considerable physiological morphological variations. Here, we describe whole-genome sequencing of aus cultivar Kasalath by using advanced next-generation (NGS) technologies to gain better understanding sequence structural changes among highly differentiated cultivars. The de novo assembled sequences represented 91.1% (330.55 Mb) genome contained 35 139 expressed...
Abstract Reproductive barriers are commonly observed in both animals and plants, which they maintain species integrity contribute to speciation. This report shows that a combination of loss-of-function alleles at two duplicated loci, DUPLICATED GAMETOPHYTIC STERILITY 1 (DGS1) on chromosome 4 DGS2 7, causes pollen sterility hybrid progeny derived from an interspecific cross between cultivated rice, Oryza sativa, Asian annual wild O. nivara. Male gametes carrying the DGS1 allele nivara...
A long awn is one of the distinct morphological features wild rice species. This organ thought to aid in seed dispersal and prevent predation by animals. Most cultivated varieties Oryza sativa glaberrima, however, have lost ability form awns. The causal genetic factors responsible for loss these two species remain largely unknown. Here, we evaluated three sets chromosome segment substitution lines (CSSLs) a common O. background (cv. Koshihikari) that harbor genomic fragments from nivara,...
Abstract Upon wounding or pathogen invasion, leaves of sorghum [Sorghum bicolor (L.) Moench] plants with the P gene turn purple, whereas recessive allele brown tan. This purple phenotype is determined by production two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced tan-phenotype plants. Using map-based cloning in progeny from a cross between Nakei-MS3B (PP) tan Greenleaf (pp) cultivars, we isolated this gene, was located 27-kb genomic region around 58.1 Mb...
Floret opening in barley is induced by the swelling of lodicule, a trait under control cleistogamy1 (cly1) gene. The product cly1 member APETALA2 (AP2) transcription factor family, which inhibits lodicule development. A sequence polymorphism at miR172 target site within has been associated with variation development and hence cleistogamous phenotype. It was unclear whether actually functions regulation and, if it does, gene contributes to cleistogamy. also interesting explore miR172-mediated...
Two species of rice have been independently domesticated from different ancestral wild in Asia and Africa. Comparison mutations that underlie phenotypic physiological alterations associated with domestication traits these gives insights into the history both regions. Asian cultivated rice, Oryza sativa, African glaberrima , modified improved for common beneficial humans, including erect plant architecture, nonshattering seeds, nonpigmented pericarp, lack awns. Independent orthologous genes...
We used an 8987-EST collection to construct a cDNA microarray system with various genomics information (full-length cDNA, expression profile, high accuracy genome sequence, phenotype, genetic map, and physical map) in rice. This array was as probe hybridize target RNAs prepared from normally grown callus of rice treated for 6 hr or 3 days the hormones abscisic acid (ABA) gibberellin (GA). identified 509 clones, including many clones that had never been annotated ABA- GA-responsive. These...
Full-length (FL) cDNA sequences provide the most reliable evidence for presence of genes in genomes. In this report, detailed gene structures barley, whole genome shotgun (WGS) and additional transcript data cultivar Haruna Nijo were quality controlled compared with published Morex information. scaffolds have longer total sequence length much higher N50 fewer than those WGS contigs. The provided efficient FLcDNA mapping, resulting high coverage detection transcription start sites....
A core collection of Japanese wheat varieties (JWC) consisting 96 accessions was established based on their passport data and breeding pedigrees. To clarify the molecular basis JWC collection, genome-wide single-nucleotide polymorphism (SNP) genotyping performed using genotyping-by-sequencing (GBS) approach. Phylogenetic tree population structure analyses these SNP revealed genetic diversity relationships among accessions, classifying them into four groups; "varieties in Hokkaido area",...
Insertion-deletion (indel) polymorphisms, such as simple sequence repeats, have been widely used DNA markers to identify QTLs and genes facilitate rice breeding. Recently, next-generation sequencing has produced deep sequences that allow genome-wide detection of indels. These polymorphisms can potentially be develop high-accuracy polymerase chain reaction (PCR)-based markers. Here, re-sequencing 5 indica, 2 aus, 3 tropical japonica cultivars Japanese elite cultivar 'Koshihikari' was...
A new YAC (yeast artificial chromosome) physical map of the 12 rice chromosomes was constructed utilizing latest molecular linkage map. The 1439 DNA markers on genetic selected a total 1892 YACs from library. 675 distinct were assigned to specific chromosomal locations. In all chromosomes, 297 contigs and 142 islands formed. length these estimated 270 Mb which corresponds approximately 63% entire genome (430 Mb). Because each contig has been measured, we could then estimate distance between...
Sorghum (Sorghum bicolor L. Moench) accumulates 3-deoxyanthocyanidins and exhibits orange to purple coloration on parts of the leaf in response infection with fungus Bipolaris sorghicola. We aimed identify key genes determining this color variation. populations derived from Nakei-MS3B M36001 accumulated apigeninidin, or both apigeninidin luteolinidin, different proportions lesions caused by B. sorghicola infection, suggesting that relative two determine QTL analysis genomic sequencing...
The mechanism underlying the interaction between host plant and host-selective toxin (HST)-producing Alternaria alternata during infection is of particular interest for sustainable crop production. blotch apple (Malus × domestica Borkh.) caused by A. pathotype a major disease particularly in East Asia, which largest producer apples globally. A single dominant gene, Alt, controls susceptibility cultivar 'Delicious' to blotch. In this study, we fine mapped Alt locus characterized three...