A5068332120- Molecular Junctions and Nanostructures
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Nanowire Synthesis and Applications
- Advanced Chemical Sensor Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Nanopore and Nanochannel Transport Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Innovative Microfluidic and Catalytic Techniques Innovation
- Graphene and Nanomaterials Applications
Georgia Institute of Technology
2017-2023
University of North Carolina at Chapel Hill
2018
Queen's University Belfast
2017
Manufacturing Institute
2017
Teagasc - The Irish Agriculture and Food Development Authority
2017
University College Cork
2017
Biosensors based on Electrochemical Impedance Spectroscopy (EIS) detect the binding of an analyte to a receptor functionalized electrode by measuring subsequent change in extracted charge-transfer resistance (R CT ). In this work, stability long chain alkanethiol, 16-mercaptohexadecanoic acid was compared that polymer-based surface linker, ortho-aminobenzoic (o-ABA). These two classes linkers were selected due marked differences their structural properties. The drift R observed for native...
A fully integrated system that combines extended gate field-effect transistor (EGFET)-based potentiometric biosensors and electrochemical impedance spectroscopy (EIS)-based has been demonstrated. This configuration enables the sequential measurement of same immunological binding event on sensing surface consequently sheds light fundamental origins signals produced by FET EIS biosensors, as well correlation between two. Detection both bovine serum albumin (BSA)/anti-BSA model in buffer...
μ-opioid receptors (MORs) in the nucleus accumbens (NAc) can regulate reward-related behaviors that are dependent on mesolimbic dopamine, but precise mechanism of this MOR regulation is unknown. We hypothesized MORs within NAc core dopamine release. Specifically, we infused antagonist CTAP (d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2) into while release was evoked by electrical stimulation ventral tegmental area and measured fast-scan cyclic voltammetry. report dose-dependently inhibited...
Across a wide range of biomarker detection schemes, carboxyl-terminated thiol self-assembled monolayers (COOH-SAMs) on Au are utilized to functionalize the sensing surface with bioreceptor via amine coupling. However, commonly used COOH-SAM preparation methods result in large defect densities due cooperative hydrogen bonding between carboxylic acid end groups, which turn leads nonselective adsorption (NSA) proteins hydrophobic surfaces exposed by these defects. In this work, X-ray...
Abstract Graphene has been utilized in sensors to detect a wide range of biomolecules (e.g. glucose, DNA, antigens, enzyme activity, dopamine) using various sensing modalities surface plasmon resonance, potentiometry, electro-impedance spectroscopy, cyclic voltammetry). However, while graphene-based biosensors have demonstrated many different architectures, little attention given the effects substrate that supports atomically thin graphene layer. In this work, we investigate protein adhesion...
Mass transport to the sensor surface is a critical step in biosensing, often being factor determining limit of detection. Modeling analyte under convection and diffusion challenging task requiring complex simulation. Herein, we provide general model for mass planar nanowire biosensors flow that applicable over wide range variables. The then used examine importance radial compared with flow. Only diffusive nanowires are found have greatly reduced settling times sensors due diffusion. However,...
Introduction Real-time bioprocess monitoring and control is needed for the scalable production deployment of cancer cell therapies at reasonable cost. For example, CAR-T therapy shows promise as an effective treatment with high (83%) remission rates. 1 However, cost these treatments too expensive their mass adoption. 2 One reason steep price difficulty scaling while ensuring delivery efficacious to patient. factor preventing scalability lack process analytical technology. As a potential...
Introduction Real-time bioprocess monitoring and control is needed for the scalable production deployment of cancer cell therapies at reasonable cost. For example, CAR-T therapy shows promise as an effective treatment with high (70-90%) remission rates. 1 However, cost these treatments too expensive their mass adoption. 2 One reason steep price difficulty scaling while ensuring delivery efficacious to patient. factor preventing scalability lack process analytical technologies. As a potential...
Theoretical models have predicted that pH-responsive surface groups can reduce the sensitivity of field-effect transistor biosensors. However, attempts to prove this experimentally shown conflicting results. In work, a graphene field effect (gFET) biosensor is used, which, without modification, pH insensitive. The then functionalized using 1-hydroxypyrene modulate gFET. A ranging from 3.9 36.8 mV/pH was demonstrated. biosensitivity gFETs tested streptavidin–biotin as model system....
The detection of chemical and biological species is critical for applications in healthcare, environmental monitoring, food safety drug screening. Potentiometric biochemical sensors rely on the specific adsorption charged analytes that changes potential functionalized sensor surface. change surface can be measured using a field-effect-transistor (FET) enabling low-cost fabrication, easy integration with portable readout electronics. FET concept has previously been applied to numerous (nano-)...