A5014676265- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Viral Infectious Diseases and Gene Expression in Insects
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Electrochemical sensors and biosensors
- CRISPR and Genetic Engineering
- Robotic Mechanisms and Dynamics
- Porphyrin and Phthalocyanine Chemistry
- Pluripotent Stem Cells Research
- Advanced Biosensing Techniques and Applications
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- Biotin and Related Studies
- Pancreatic function and diabetes
- Microbial Fuel Cells and Bioremediation
- Mechanical Engineering and Vibrations Research
- Advanced Fluorescence Microscopy Techniques
- MicroRNA in disease regulation
- Mechatronics Education and Applications
- Mechanics and Biomechanics Studies
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
Hiroshima University
2016-2025
Higashihiroshima Medical Center
2014-2019
Moscow State University of Medicine and Dentistry
2017
The University of Western Australia
2017
Tokyo Institute of Technology
1997-2014
Tokyo University of Agriculture and Technology
2009-2013
Cornell University
2007-2012
Ehime University
2007
University of Florida
2007
Honda (Japan)
2004
Abstract Glucosylation of anthocyanin in carnations (Dianthus caryophyllus) and delphiniums (Delphinium grandiflorum) involves novel sugar donors, aromatic acyl-glucoses, a reaction catalyzed by the enzymes acyl-glucose–dependent 5(7)-O-glucosyltransferase (AA5GT AA7GT). The AA5GT enzyme was purified from carnation petals, cDNAs encoding Dc delphinium homolog Dg AA7GT were isolated. Recombinant proteins showed activities vitro. Although expression developing petals highest at early stages,...
Water-soluble cyclodextrin (CyD) complexed with porphyrin derivatives different substituents in the meso-positions showed photodynamic activities toward cancer cells under illumination at wavelengths over 600 nm, most suitable for therapy (PDT). In particular, aniline- and phenol-substituted had high activity because of efficient intracellular uptake complexes by tumor cells. These greater than photofrin, currently main drug clinical use as a photosensitizer. results represent significant...
Abstract There is a growing demand to develop biocontainment strategies that prevent unintended proliferation of genetically modified organisms in the open environment. We found hypophosphite (H 3 PO 2 , HPt) transporter HtxBCDE from Pseudomonas stutzeri WM88 was also capable transporting phosphite Pt) but not phosphate 4 Pi), suggesting potential for engineering Pt/HPt-dependent bacterial strain as strategy. disrupted all Pi and organic transporters an Escherichia coli expressing HtxABCDE...
Recent progress in genetic engineering and synthetic biology have greatly expanded the production capabilities of cyanobacteria, but concerns regarding biosafety issues risk contamination cultures outdoor culture conditions remain to be resolved. With this dual goal mind, we applied recently established biological containment strategy based on phosphite (H3PO3, Pt) dependency model cyanobacterium Synechococcus elongatus PCC 7942 ( Syn 7942). Pt assimilation capability was conferred by...
Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein system using universal adapter interface between IgG DNA-modified reporter molecules. As demonstration this capability, successfully used DNA nano-barcodes, quantum dots, horseradish peroxidase enzyme detect multiple...
[60]Fullerene (C60) derivatives were incorporated into liposomes using a fullerene exchange method involving the transfer of from cavity two γ-cyclodextrin molecules to liposome. A lipid-membrane-incorporated C60 derivative bearing polar group showed much higher photodynamic activity than analogous system incorporating pristine C60.
A preparation of a lipid-membrane-incorporated tetraphenylporphyrin was achieved from the corresponding tetraphenylporphyrin·cyclodextrin complexes using an exchange method in both liposomes and cells.
It is currently not clear how to quantifiably relate pore-scale observations of colloid transport larger scales, so, we proposed a geometric theory showing that pore-scale-derived rate constants may be appropriate model scale system. This study considered three different types colloids: latex microspheres, Escherichia coli, and microspheres made poly lactic acid (PLA). Colloid attachment detachment were calculated using digital microscope images, taken in rapid (1 s) sequences, from which...
Frustrated phagocytosis occurs when an asbestos fiber > 10 μm in length is engulfed imperfectly by a macrophage, and it believed to be associated with chromosomal instability. Few studies have focused on dynamic cellular imaging assess the toxicity of hazardous inorganic materials such as asbestos. One reason for this relative lack fluorescent probes available facilitate experimental visualization materials. We recently developed asbestos-specific based asbestos-binding proteins, achieved...
The weak absorbance of pristine C60 , C70 and fullerene derivatives at wavelengths over 600 nm hampers the use these molecules as photosensitizers (PSs) for photodynamic therapy (PDT). coexistence light-harvesting antenna with a derivative in lipid membrane bilayers solved this issue. By controlling location membrane, liposomal dyad system PDT improved activity via an efficient photoenergy transfer from to derivative. was found be much higher than those systems using .
A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a signal and works single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure open to closed states produces FRET in response vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 pre-fixed model expressed inside living...