A5052847980- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Porphyrin and Phthalocyanine Chemistry
- Supramolecular Chemistry and Complexes
- RNA Interference and Gene Delivery
- Antimicrobial agents and applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Polydiacetylene-based materials and applications
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- Surfactants and Colloidal Systems
- Alzheimer's disease research and treatments
- Nanoparticle-Based Drug Delivery
- Molecular Junctions and Nanostructures
- Molecular Sensors and Ion Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Synthesis and Properties of Aromatic Compounds
- Fullerene Chemistry and Applications
- Photoreceptor and optogenetics research
- Graph theory and applications
- Photochromic and Fluorescence Chemistry
- Advanced Polymer Synthesis and Characterization
- Luminescence and Fluorescent Materials
- Force Microscopy Techniques and Applications
Nara Institute of Science and Technology
2016-2025
National Archives and Records Administration
2017-2019
PRG S&Tech (South Korea)
2018
University of California, San Francisco
2015
Osaka University
2006
Kanazawa University
2005
The increasing prevalence of antibiotic-resistant bacterial infections, coupled with the decline in number new antibiotic drug approvals, has created a therapeutic gap that portends an emergent public health crisis.
There is an urgent need for new antibiotics which are effective against drug‐resistant bacteria without contributing to resistance development. We have designed and developed antimicrobial copolymers with cationic amphiphilic structures based on the mimicry of naturally occurring peptides. These exhibit potent activity a broad spectrum including methicillin‐resistant Staphylococcus aureus no adverse hemolytic activity. Notably, these polymers also did not result in any measurable development...
There is a growing interest in the use of lipid bilayer nanodiscs for various biochemical and biomedical applications. Among different types nanodiscs, unique features synthetic polymer-based have attracted additional interest. A styrene–maleic acid (SMA) copolymer demonstrated to form has been used structural biology related studies on membrane proteins. However, application SMA polymer based limited because strong absorption aromatic group interfering with experimental measurements. Thus,...
Herein, we report the synthesis and planar chiral properties of a pair water-soluble cationic pillar[5]arenes with stereogenic carbons. Interestingly, although units molecules were rotatable, only one diastereomer existed in water both cases. As new type source, these transmitted information from cavities to assembly extended π-conjugated compound, affording circularly polarized luminescence (CPL). The chirality transfer process resulting CPL extremely sensitive feed ratio owing combined...
Membrane protein integrase (MPIase) is a glycolipid found in Escherichia coli cell membranes. It consists of diacylglycerol and sugar chain comprising approximately 10 repeating trisaccharide units, made up three types N-acetylated amino sugars linked by pyrophosphate. In cooperation with proteinaceous translocons, MPIase regulates membrane integration. this study, using various microscopic techniques, we demonstrated that externally added induces the formation single tubule protruding...
Polymethacrylate-copolymer (PMA) encased lipid-nanodiscs modulate Aβ<sub>1–40</sub> aggregation and neurotoxicity.
In humans, β-amyloid and islet amyloid polypeptide (IAPP, also known as amylin) aggregations are linked to Alzheimer's disease type-2 diabetes, respectively. There is significant interest in better understanding the aggregation process by using chemical tools. Here, we show ability of a cationic polymethacrylate-copolymer (PMAQA) quickly induce β-hairpin structure accelerate formation amorphous aggregates β-amyloid-1-40, whereas it constrains conformational plasticity amylin for several days...
Abstract The behavior of self‐assembly processes nanoscale particles on plasma membranes can reveal mechanisms important biofunctions and/or intractable diseases. Self‐assembly citrate‐coated gold nanoparticles (cAuNPs) liposomes was investigated. adsorbed cAuNPs were initially fixed the liposome surfaces and did not self‐assemble below phospholipid phase transition temperature ( T m ). In contrast, anisotropic cAuNP observed upon heating composite above , where phospholipids became fluid....
The function and mode of action curcumin in modulating the formation lipid raft domains were investigated by microscopic observation using model membranes. Curcumin induces fusion at extremely low concentrations through alteration boundary between ordered disordered phases.
The discovery of anticancer therapeutics effective in eliminating dormant cells is a significant challenge cancer biology. Here, we describe new synthetic polymer-based agents that mimic the mode action peptides. These polymers developed here are designed to capture cationic, amphiphilic traits target anionic lipids exposed on cell surfaces and act by disrupting membranes. Because polymer mechanism not dependent proliferation, hypothesized were active against cells. exhibited cytotoxicity...
Mixing cyclic pentagonal pillar[5]quinone with hexagonal pillar[6]arene in a 12:20 molar feed ratio resulted spontaneous production of vesicles, while assembly and alone produced disks wires, respectively. Incorporation rings into sheets gave curvature contributed to the formation vesicles. Conventional vesicles are generally synthesized by amphiphilic molecules containing hydrophobic hydrophilic parts. Therefore, co-assembly obtain spherical demonstrated this study is new concept based on...
Cell-secreted vesicles, such as exosomes, have recently been recognized mediators of cell communication. A recent study in cattle showed the involvement exosome-like vesicles control cumulus expansion, a prerequisite process for normal ovulation; however, whether this is case other mammalian species not known. Therefore, aimed to examine presence ovarian follicles and their effects on expansion vitro pigs. The porcine follicular fluid (pFF) was confirmed by transmission electron microscopic...
Constructing binding pockets by hierarchically assembling tailored building blocks and understanding structure-property relationships are challenging goals. Herein, amphiphilic pillar[5]arene pillar[6]arene were prepared used to construct 2D sheets, which consisted of well-defined hydrophobic hydrophilic interlayers. In the interlayers, internal created packing pairs pillar[n]arenes, external simultaneously generated from gaps between pillar[n]arenes due electrostatic attractions. Aromatic...
Well-defined hybrid nanodiscs were produced by employing bicelle formation of a binary lipid mixture in water. The resulting nanoparticles have bilayer coated with ceramic layers, which are formed the sol–gel reaction among alkoxysilyl headgroups lipid. bicelles displayed significant morphological stability against dry environments and surfactant addition, stark contrast to conventional phospholipid bicelles.
CD44 is a transmembrane cell adhesion molecule that cleaved by the membrane proteinase, disintegrin and metalloproteinase 10 (ADAM10), on surface via ectodomain shedding after cholesterol depletion. Lipid raft-mediated essential for cancer invasion. As cell–cell cell–matrix adhesions are critical progression, lipid raft-targeting agents may be effective therapy. Here, we found curcumin its derivatives induced ADAM10-mediated of in tumor cells. We also membrane-active compounds whose effect...
Abstract Lipid‐membrane‐incorporating C 60 and 70 (LMIC LMIC ) were prepared by the fullerene‐exchange reaction from γ‐cyclodextrin cavity to vesicles (we call this method “exchange method”). An advantage of is that ratios [C ]/[lipids] can be arbitrarily controlled adjusting fullerenes liposome. The maximum ratio (30 mol %) obtained was approximately 14 100 times higher than those achieved for , respectively, classical method, which we “premixing method” (dissolving lipids or in chloroform,...
A preparation of a lipid-membrane-incorporated tetraphenylporphyrin was achieved from the corresponding tetraphenylporphyrin·cyclodextrin complexes using an exchange method in both liposomes and cells.
This highlight review describes our contribution to the field of biomimetic and technomimetic molecular machines specially designed be studied as single molecules on surfaces. Prototypes wheels, vehicles, gears motors will described from their design study controlled motions using ultra-high-vacuum low-temperature scanning tunneling microscopes (UHV-LT-STM). in microscopes.
Lipid nanodisc is a promising nano-carrier for molecular delivery that efficiently delivers hydrophobic molecules to intact cells.
The activities of biomolecules are affected by the proton concentrations at biological membranes. Here, we succeeded in evaluating interface concentration (-log[H+] defined as pH') cardiolipin (CL)-enriched membrane models inner mitochondrial (IMM) using a spiro-rhodamine-glucose molecule (RHG). According to fluorescence microscopy and 1H-NMR studies, RHG interacted with Stern layer membrane. acid/base equilibrium between its protonated open form (o-RHG) deprotonated closed spiro-form...
Cationic amphiphilic polymers have been a platform to create new antimicrobial materials that act by disrupting bacterial cell membranes. While activity characterization and chemical optimization done in numerous studies, there remains gap our knowledge on the mechanisms of polymers, which is needed connect their structures biological activities. To end, we used single giant unilamellar vesicle (GUV) method identify membrane-disrupting mechanism methacrylate random copolymers. The copolymers...