A5014605284- Amyotrophic Lateral Sclerosis Research
- DNA Repair Mechanisms
- Urologic and reproductive health conditions
- Prion Diseases and Protein Misfolding
- Bacterial Genetics and Biotechnology
- Hormonal and reproductive studies
- Neurogenetic and Muscular Disorders Research
- Sperm and Testicular Function
- Chemical Reactions and Isotopes
- Prostate Cancer Treatment and Research
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Advanced Proteomics Techniques and Applications
- Plant Genetic and Mutation Studies
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Cholinesterase and Neurodegenerative Diseases
- Neurological diseases and metabolism
- biodegradable polymer synthesis and properties
- Cytokine Signaling Pathways and Interactions
- Alzheimer's disease research and treatments
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Cleft Lip and Palate Research
Central University of South Bihar
2016-2024
University of Massachusetts Chan Medical School
2013
Jawaharlal Nehru University
2007-2011
International Centre for Genetic Engineering and Biotechnology
2007
Replication initiation is a crucial step in genome duplication and homohexameric DnaB helicase plays central role the replication process by unwinding duplex DNA interacting with several other proteins during of replication. N-terminal domain critical for activity DnaG primase interactions. We present here crystal structure (NTD) H. pylori (HpDnaB) at 2.2 Å resolution compare structural differences among helicases correlate functional differences. The details NTD suggest that linker region...
Human seminal plasma contains a large array of proteins required for the normal physiology and metabolism spermatozoa. These are mainly secreted from prostate epithelium, testes, vesicles. Fortunately, many these found to be present at elevated concentration in act as biomarker different carcinomas their levels also enhanced serum involved tumor progression metastasis apart fertility. The which were overexpressed carcinoma patients identified by 2-DE MALDI-TOF/MS. We have designed strategy...
Abstract Human seminal plasma (HuSP) contains several proteins that bind heparin and related glycosaminoglycans. Heparin binding (HBPs) from have been shown to participate in modulation of capacitation or acrosome reaction thus correlated with fertility some species. However, these not studied detail human. The objective this study was purify major HBPs HuSP order characterize proteins. were isolated by affinity—chromatography on Heparin—Sepharose column, purified reverse‐phase...
Hexameric DnaB type replicative helicases are essential for DNA strand unwinding along with the direction of replication fork movement. These in general contain an amino terminal domain and a carboxy separated by linker region. Due to lack crystal structure full-length like helicase, function these types not clear. We have reported recently that Helicobacter pylori helicase is vitro it can bypass Escherichia coli DnaC activity vivo . Using biochemical, biophysical genetic complementation...
TDP-43 proteinopathies is a disease hallmark that characterizes amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The N-terminal domain of (NTD) important to both physiology proteinopathy. However, its folding dimerization process still poorly characterized. In the present study, we have investigated folding/unfolding NTD employing all-atom molecular dynamics (MD) simulations in 8 M dimethylsulfoxide (DMSO) at high temperatures. MD results showed unfolding...
Entamoeba histolytica, the causative agent of human amoebiasis, is essentially anaerobic, requiring a small amount oxygen for growth. It cannot tolerate higher concentration present in tissues or blood. However, during tissue invasion it exposed to level oxygen, leading stress. Cysteine, which vital thiol E. plays an essential role its oxygen-defence mechanisms. The major route cysteine biosynthesis this parasite condensation O-acetylserine with sulfide by de novo cysteine-biosynthetic...
The clam Scapharca inaequivalvis possesses two cooperative oxygen binding hemoglobins in its red cells: a homodimeric HbI and heterotetrameric A2B2 HbII. Each AB dimeric half of HbII is assembled manner very similar to that the well-studied HbI. This study presents crystal structures along with data both crystalline state wet nanoporous silica gels. Despite ligand-linked structural transitions observed crystals, or encapsulated gels apparently exhibits minimal cooperativity binding, contrast...
Abstract The seminal plasma comprises secretions from various accessory sex glands. During fertilization spermatozoa undergo complex sequences of precisely timed events that are regulated by the activation different intracellular signaling pathways. precision and efficacy these pathways often influenced assembly interactions multiprotein complexes, thereby directing flow regulatory information. Our knowledge about protein complexes present in human (HuSP) is limited. Here we report...