A5083114048- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Monoclonal and Polyclonal Antibodies Research
- Angiogenesis and VEGF in Cancer
- Wnt/β-catenin signaling in development and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Enhanced Recovery After Surgery
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Platelet Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Urticaria and Related Conditions
- Caveolin-1 and cellular processes
- Nitric Oxide and Endothelin Effects
- S100 Proteins and Annexins
- Pancreatic and Hepatic Oncology Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Cell Function and Interaction
- Congenital heart defects research
- T-cell and B-cell Immunology
- Autoimmune Bullous Skin Diseases
- Colorectal Cancer Surgical Treatments
Amsterdam University Medical Centers
2018-2022
Vrije Universiteit Amsterdam
2020-2022
The Netherlands Cancer Institute
2021
Cancer Center Amsterdam
2018
Amsterdam UMC Location Vrije Universiteit Amsterdam
2018
University of Amsterdam
2014-2017
Sanquin
2014-2017
Amsterdam UMC Location University of Amsterdam
2014-2017
Abstract During immune surveillance and inflammation, leukocytes exit the vasculature through transient openings in endothelium without causing plasma leakage. However, exact mechanisms behind this intriguing phenomenon are still unknown. Here we report that maintenance of endothelial barrier integrity during leukocyte diapedesis requires local RhoA cycling. Endothelial depletion vitro or Rho inhibition vivo provokes neutrophil-induced vascular leakage manifests physical movement neutrophils...
Endothelial cell-cell junctions maintain a restrictive barrier that is tightly regulated to allow dynamic responses permeability-inducing angiogenic factors as well inflammatory agents and adherent leukocytes. The ability of these stimuli transiently remodel adherens (AJs) depends on Rho-GTPase-controlled cytoskeletal rearrangements. How activity Rho-GTPases spatio-temporally controlled at endothelial AJs by guanine-nucleotide exchange (GEFs) incompletely understood. Here, we identify...
Most clinically used anticancer mAbs are of the IgG isotype, which can eliminate tumor cells through NK cell–mediated antibody-dependent cellular cytotoxicity and macrophage-mediated phagocytosis. IgG, however, ineffectively recruits neutrophils as effector cells. IgA induce migration activation Fc receptor (FcαRI) but unable to activate have poorer half-life. Here, we combined agonistic activity FcαRI targeting in a therapeutic bispecific antibody format. The resulting TrisomAb molecules...
Endothelial cells line the lumen of vessel wall and are exposed to flow. In linear parts vessel, endothelial experience laminar flow, resulting in cell alignment direction thereby protecting from inflammation permeability. order for align, they undergo rapid remodeling actin cytoskeleton by local activation small GTPase Rac1. However, it is not clear whether sustained Rac1 required long-term flow-induced alignment. Using a FRET-based DORA biosensor, we show that activity remains 12 h upon...
Abstract ICAM-1 is required for firm adhesion of leukocytes to the endothelium. However, how spatial organization endothelial regulates leukocyte not well understood. In this study, we identified calcium-effector protein annexin A2 as a novel binding partner ICAM-1. clustering promotes ICAM-1–annexin interaction and induces translocation into caveolin-1–rich membrane domains. Depletion using RNA interference enhances mobility prevents Surprisingly, results in increased neutrophil...
Immunoglobulin A (IgA) is generally considered as a non-inflammatory regulator of mucosal immunity, and its importance in diversifying the gut microbiota increasingly appreciated. IgA autoantibodies have been found several autoimmune or chronic inflammatory diseases, but their role pathophysiology ill-understood. can interact with Fc receptor FcαRI on immune cells. We now established novel blistering model, which closely resembles human disease linear bullous (LABD) by using genetically...
Surgical resection of the primary tumor provides best chance cure for patients with colorectal carcinoma (CRC). However, bacterial translocation during intestinal surgery has been correlated poor long-term oncological outcome. Therefore, we investigated influence contamination colon on CRC liver metastases development. Blood and samples undergoing or were collected. Cell numbers, activation markers inflammatory mediators determined. Tumor cell adhesion outgrowth after sham- colectomy...
Abstract In epithelial cancers, the epidermal growth factor receptor (EGFR) and integrin α6β4 are frequently overexpressed found to synergistically activate intracellular signaling pathways that promote cell proliferation migration. cancer cells, β4 subunit is phosphorylated at tyrosine residues not normally recognized as kinase substrates; however, function of these phosphotyrosine in cells a subject much debate. EGFR-overexpressing carcinoma we Src family (SFK) inhibitor PP2 reduces...
Endothelial cell-cell junctions maintain a restrictive barrier that is tightly regulated to allow dynamic responses permeability-inducing angiogenic factors, as well inflammatory agents and adherent leukocytes. The ability of these stimuli transiently remodel adherens depends on Rho-GTPase-controlled cytoskeletal rearrangements. How the activity Rho-GTPases spatio-temporally controlled at endothelial by guanine-nucleotide exchange factors (GEFs) incompletely understood. Here, we identify...