A5056768784- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Thyroid Cancer Diagnosis and Treatment
- melanin and skin pigmentation
- Hippo pathway signaling and YAP/TAZ
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- Fullerene Chemistry and Applications
- Graphene research and applications
- Light effects on plants
- Salivary Gland Tumors Diagnosis and Treatment
- Cellular transport and secretion
- Hair Growth and Disorders
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Carbon Nanotubes in Composites
- Wnt/β-catenin signaling in development and cancer
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
Soochow University
2025
Third People's Hospital of Hangzhou
2022-2024
Hebei University of Technology
2024
Peking University
1996-2024
Guilin Medical University
2024
UConn Health
2011-2023
Fudan University Shanghai Cancer Center
2001-2021
University of Connecticut
2015-2021
Shanghai Medical College of Fudan University
2010-2020
Sun Yat-sen University
2005-2018
Vimentin intermediate filaments (VIF) extend throughout the rear and perinuclear regions of migrating fibroblasts, but only nonfilamentous vimentin particles are present in lamellipodial regions. In contrast, VIF networks to entire cell periphery serum-starved or nonmotile fibroblasts. Upon serum addition activation Rac1, rapidly phosphorylated at Ser-38, a p21-activated kinase phosphorylation site. This is coincident with disassembly retraction from surface where lamellipodia form....
By proteolytic modification of low abundant signaling proteins and membrane receptors, proteases exert potent posttranslational control over cell behavior at the postsecretion level. Hence, substrate discovery is indispensable for understanding biological role in vivo . Indeed, matrix metalloproteinases (MMPs), long associated with extracellular degradation, are increasingly recognized as important processing enzymes bioactive molecules. MS now primary proteomic technique detecting,...
Abstract During immune surveillance and inflammation, leukocytes exit the vasculature through transient openings in endothelium without causing plasma leakage. However, exact mechanisms behind this intriguing phenomenon are still unknown. Here we report that maintenance of endothelial barrier integrity during leukocyte diapedesis requires local RhoA cycling. Endothelial depletion vitro or Rho inhibition vivo provokes neutrophil-induced vascular leakage manifests physical movement neutrophils...
Abstract The small GTPase RhoA is involved in cell morphology and migration. activity tightly regulated time space depends on guanine exchange factors (GEFs). However, the kinetics subcellular localization of GEF towards are poorly defined. To study mechanism underlying spatiotemporal control by GEFs, we performed single imaging with an improved FRET sensor reporting nucleotide loading state RhoA. By employing show that a plasma membrane located RhoGEF, p63RhoGEF, can rapidly activate...
Endothelial cell-cell junctions maintain a restrictive barrier that is tightly regulated to allow dynamic responses permeability-inducing angiogenic factors as well inflammatory agents and adherent leukocytes. The ability of these stimuli transiently remodel adherens (AJs) depends on Rho-GTPase-controlled cytoskeletal rearrangements. How activity Rho-GTPases spatio-temporally controlled at endothelial AJs by guanine-nucleotide exchange (GEFs) incompletely understood. Here, we identify...
Type I collagen stimulation of pro-matrix metalloproteinase (pro-MMP)-2 activation by ovarian cancer cells involves β1 integrin receptor clustering; however, the specific cellular and biochemical events that accompany MMP processing are not well characterized. Collagenolysis is required for pro-MMP-2 activation, denatured does elicit an MMP-2 response. Similarly, DOV13 bind to intact utilizing both α2β1 α3β1 integrins but interact poorly with collagenase-treated or thermally collagen....
Up-regulation of the collagenolytic membrane type-1 matrix metalloproteinase (MT1-MMP) leads to increased MMP2 (gelatinase A) activation and MT1-MMP autolysis. The autocatalytic degradation product is a cell surface 44-kDa fragment (Gly285–Val582) in which ectodomain consists only linker, hemopexin C domain stalk segment found before transmembrane sequence. In collagenases, exosites bind native collagen, required for triple helicase activity during collagen cleavage. Here we investigated...
Acquisition of matrix metalloproteinase-2 (MMP-2) activity is temporally associated with increased migration and invasiveness cancer cells. ProMMP-2 activation requires multimolecular complex assembly involving proMMP-2, membrane type 1-MMP (MT1-MMP, MMP-14), tissue inhibitor metalloproteinases-2 (TIMP-2). Because transforming growth factor-beta1 (TGF-beta1) promotes tumor invasion in advanced squamous cell carcinomas, the role TGF-beta1 regulation MMP a cellular model invasive oral...
Amino acid esters react with C60 both thermally and photochemically to give different products. Refluxing a mixture of glycine ethyl ester afforded C60(Me2CHNHCHCOOEt) 1, whereas irradiation the same produced C60(EtOOCCHNHCHCOOEt) 2b as main product. Photochemical reactions between sarcosine yielded two pyrrolidine derivatives C60(CH2N(Me)CHCOOR) 3 C60(ROOCCHNHCHCOOR) 2 (R = Me, Et, CH2Ph). Compound 2a is also prepared from photochemical reaction iminodiacetic methyl in high yield. These are...
Directed cell migration in native environments is influenced by multiple migratory cues. These cues may include simultaneously occurring attractive soluble growth factor gradients and repulsive effects arising from cell–cell contact, termed contact inhibition of locomotion (CIL). How single cells reconcile potentially conflicting remains poorly understood. Here we show that a dynamic crosstalk between epidermal (EGF)-mediated chemotaxis CIL guides metastatic breast cancer motility, whereby...
Polarized Rac1 signaling is a hallmark of many cellular functions, including cell adhesion, motility, and division. The two steps activation are its translocation to the plasma membrane exchange nucleotide from GDP GTP. It is, however, unclear whether these processes regulated independent each other what their respective roles in polarization signaling. We designed single-particle tracking (SPT) method quantitatively analyze kinetics living cells. found that rate was significantly elevated...
Conclusion: Chinese patients have a higher rate of lymphoepithelial carcinoma (LEC) and salivary duct (SDC). Comprehensive use diagnostic modalities, neck dissection, postoperative radiation will improve the treatment results for gland tumors (SGTs). Objectives: To study clinicopathological characteristics SGTs in population. Methods: The records SGT operated tertiary cancer hospital China were retrieved. Results: From December 1997 to 2007, 289 malignant 887 benign at Cancer Hospital,...
The bioactive sphingosine-1-phosphatephosphate (S1P) is present in plasma, bound to carrier proteins, and involved many physiological processes, including angiogenesis, inflammatory responses, vascular stabilization. S1P can bind several G-protein-coupled receptors (GPCRs) activating a number of different signaling networks. At present, the dynamics relative importance events activated immediately downstream GPCR activation are unclear. To examine these, we used set fluorescence resonance...
Bipolar disorder, schizophrenia, autism and intellectual disability are complex neurodevelopmental disorders, debilitating millions of people. Therapeutic progress is limited by poor understanding underlying molecular pathways. Using a targeted search, we identified an enrichment de novo mutations in the gene encoding 330-kDa triple functional domain (TRIO) protein associated with disorders. By generating multiple TRIO antibodies, show that smaller TRIO9 isoform major brain product, its...
Endothelial cells line the vasculature and are important for regulation of blood pressure, vascular permeability, clotting transendothelial migration leukocytes tumor cells. A group proteins that control endothelial barrier function RhoGTPases. This study focuses on three homologous (>88%) RhoGTPases: RhoA, RhoB, RhoC which RhoB have been poorly characterized. Using a RhoGTPase mRNA expression analysis we identified as highest expressed in primary human Based an existing RhoA FRET sensor...
ABSTRACT Eukaryotic cells are sensitive to mechanical forces they experience from the environment. The process of mechanosensation is complex, and involves elements such as cytoskeleton active contraction myosin motors. Ultimately, connected changes in gene expression cell, known mechanotransduction. While involvement known, processes upstream cytoskeletal unclear. In this paper, by using a microfluidic device that mechanically compresses live cells, we demonstrate Ca2+ currents membrane...