A5052553351- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Plant Pathogens and Fungal Diseases
- Studies on Chitinases and Chitosanases
- Polyamine Metabolism and Applications
- Fungal Biology and Applications
- Bioenergy crop production and management
- Seaweed-derived Bioactive Compounds
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Polysaccharides and Plant Cell Walls
- Enzyme Structure and Function
- Marine Sponges and Natural Products
- Calcium Carbonate Crystallization and Inhibition
- Glycosylation and Glycoproteins Research
- Mycotoxins in Agriculture and Food
- Probiotics and Fermented Foods
- Microbial Applications in Construction Materials
- Advanced Cellulose Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
Petersburg Nuclear Physics Institute
2007-2023
Kurchatov Institute
2014-2023
KTH Royal Institute of Technology
2007
North Carolina State University
2007
AlbaNova
2007
Organization of glycoside hydrolase (GH) families into clans expands the utility information on catalytic mechanisms member enzymes. This issue was examined for GH27 and GH36 through biochemical analysis α-galactosidase from Thermotoga maritima (TmGalA). Catalytic residues in TmGalA were inferred structural homology with members to facilitate design site-directed mutants. Product confirmed that wild type (WT) acted retention anomeric stereochemistry, analogous Conserved acidic kinetic D327G...
The ascomycete Geotrichum candidum is a versatile and efficient decay fungus that involved, for example, in biodeterioration of compact discs; notably, the 3C strain was previously shown to degrade filter paper cotton more efficiently than several industrial enzyme preparations. Glycoside hydrolase (GH) family 7 cellobiohydrolases (CBHs) are primary constituents cellulase cocktails employed biomass conversion, feature tunnel-enclosed active sites enable processive hydrolytic cleavage...
Microbially induced CaCO3 precipitation (MICP) is considered as an alternative green technology for cement self-healing and a basis the development of new biomaterials. However, some issues about role bacteria in induction biogenic crystal nucleation, growth aggregation are still debatable. Our aims were to screen ureolytic calcifying microorganisms analyze their MICP abilities during urea-supplemented urea-deficient media. Nine candidates showed high level urease specific activity, sharp...
Enzymes capable of modifying the sulfated polymeric molecule fucoidan are mainly produced by different groups marine organisms: invertebrates, bacteria, and also some fungi. We have discovered identified a new strain filamentous fungus Fusarium proliferatum LE1 (deposition number in Russian Collection Agricultural Microorganisms is RCAM02409), which potential producer fucoidan-degrading enzymes. The (RCAM02409) was on basis morphological characteristics analysis ITS sequences ribosomal DNA....
We report here the draft genome sequence of Geotrichum candidum strain 3C, which is a filamentous yeast-like fungus that holds great promise for biotechnology. The was sequenced using Ion Torrent and 454 platforms. estimated size 41.4 Mb, 14,579 protein-coding genes were predicted ab initio.
In the 1970s, strain Geotrichum candidum Link 3C was isolated from rotting rope and since then has been extensively studied as a source of cellulose xylan‐degrading enzymes. The original identification based only on morphological characters fungal mycelium in culture. Recent comparison internal transcribed spacer (ITS) fragments derived draft genome published 2015 did not show its similarity to G. species. Given value lignocellulosic biomass degradation, we performed molecular studies find...
Abstract Synergistic action of exo‐ and endohydrolazes is preferred for effective destruction biopolymers. The main purpose the present work was to develop an efficient tool degradation xylan. Macroporous lab‐made monolithic columns commercial CIM‐Epoxy disk were used immobilize recombinant β ‐xylosidase from Aspergillus awamori Grindamyl ‐xylanase. efficiency xylan using low‐loaded column appeared be four times higher than in‐solution process about six high‐loaded bioreactor. Disk...
Sulfatases are a family of enzymes (sulfuric ester hydrolases, EC 3.1.6.-) that catalyze the hydrolysis wide array sulfate esters. To date, despite discovery many sulfatase genes and accumulation data on numerous sulfated molecules, number characterized key players in sulfur metabolism remains extremely limited. While mammalian sulfatases well studied due to their involvement range normal pathological biological processes, lower eukaryotic sulfatases, especially fungal have not been...
Xanthan is an extracellular heteropolysaccharide produced by the bacteria Xanthomonas campestris. Due to its unique properties, polysaccharide and derivatives are widely used in many industries, from food biomedicine oil production, that demands efficient xanthan depolymerization method adapt this for various applications. Unlike known chemical approaches, biological methods considered be more environmentally friendly less energy intensive. In laboratory conditions, we have isolated a...
Sulfatases are a family of enzymes (sulfuric ester hydrolases, EC 3.1.6.-) that catalyze the hydrolysis wide array sulfate esters. To date, despite discovery many sulfatase genes and accumulation data on numerous sulfated molecules, number characterized key players in sulfur metabolism remains extremely limited. While mammalian sulfatases well studied due to their involvement range normal pathological biological processes, lower eukaryotic sulfatases, especially fungal have not been...
Abstract Endo‐β‐xylanases are hemicellulases involved in the conversion of xylans plant biomass. Here, we report a novel acidophilic β‐xylanase (ScXynA) with high transglycosylation abilities that was isolated from filamentous fungus Scytalidium candidum 3C. ScXynA identified as glycoside hydrolase family 10 (GH10) dimeric protein, molecular weight 38 ± 5 kDa per subunit. The enzyme catalyzed hydrolysis different under acidic conditions and stable pH range 2.6–4.5. kinetic parameters were...