A5084567688- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Cannabis and Cannabinoid Research
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- Copper-based nanomaterials and applications
- Advanced Chemical Physics Studies
- Plant and Biological Electrophysiology Studies
- Bacterial Genetics and Biotechnology
- Crystallization and Solubility Studies
- Antibiotic Use and Resistance
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Nanopore and Nanochannel Transport Studies
- Parasitic Diseases Research and Treatment
- Mechanical Engineering and Vibrations Research
- nanoparticles nucleation surface interactions
- Gear and Bearing Dynamics Analysis
- Semiconductor materials and devices
- Advanced battery technologies research
- Advanced Memory and Neural Computing
- Crystallography and molecular interactions
- Cardiomyopathy and Myosin Studies
Science for Life Laboratory
2020-2023
KTH Royal Institute of Technology
2020-2023
Max Planck Institute for Developmental Biology
2020
Bhabha Atomic Research Centre
2017
Indian Institute of Science Education and Research, Bhopal
2017
Indian Institute of Science Bangalore
2014
Cannabidiol (CBD) is the primary nonpsychotropic phytocannabinoid found in Cannabis sativa, which has been proposed to be therapeutic against many conditions, including muscle spasms. Among its putative targets are voltage-gated sodium channels (Navs), have implicated conditions. We investigated effects of CBD on Nav1.4, skeletal Nav subtype. explored direct effects, involving physical block pore, as well indirect modulation membrane elasticity that contributes inhibition. MD simulations...
Voltage-gated sodium channels are heterotetrameric selective ion that play a central role in electrical signaling excitable cells. With recent advances structural biology, structures of eukaryotic have been captured several distinct conformations corresponding to different functional states. The secondary structure the pore lining S6 helices subunits DI, DII, and DIV has with both short π-helix stretches fully α-helical conformations. relevance these elements for gating is not yet...
The performance of automated protein model building usually decreases with resolution, mainly owing to the lower information content experimental data. This calls for a more elaborate use available structural about macromolecules. Here, new method is presented that uses homologues improve quality models automatically constructed using ARP / wARP . local similarity between deposited and being built, results in longer main-chain fragments turn can be reliably docked sequence. application...
Significance The cardiac sodium channel (Na v 1.5) is crucial for generating a regular heartbeat. It thus not surprising that Na 1.5 mutations have been linked to life-threatening arrhythmias. Interestingly, activity can also be altered by posttranslational modifications, such as tyrosine phosphorylation. Our combination of protein engineering and molecular modeling has revealed the detrimental effect long QT3 patient mutation only exposed when proximal phosphorylated. This suggests dynamic...
The K V 7.4 and 7.5 subtypes of voltage-gated potassium channels play a role in important physiological processes such as sound amplification the cochlea adjusting vascular smooth muscle tone. Therefore, mechanisms that regulate channel function are interest. Here, we study effect polyunsaturated fatty acids (PUFAs) on human expressed Xenopus oocytes. We report PUFAs facilitate activation hK by shifting 50 conductance versus voltage (G(V)) curve toward more negative voltages. This response...
Voltage-gated sodium (Nav) channels play critical roles in propagating action potentials and otherwise manipulating ionic gradients excitable cells. These open response to membrane depolarization, selectively permeating ions until rapidly inactivating. Structural characterization of the gating cycle this channel family has proved challenging, particularly due transient nature state. A structure from bacterium Magnetococcus marinus Nav (NavMs) was initially proposed be open, based on its pore...
Voltage-gated sodium channels play an important role in electrical signaling excitable cells. In response to changes membrane potential, they cycle between nonconducting and conducting conformations. With recent advances structural biology, structures of have been captured several distinct conformations, which are thought represent different functional states. However, it has difficult capture the intrinsically transient open state. We recently showed that a proposed state bacterial channel...
Abstract The voltage-gated sodium channel Na v 1.5 initiates the cardiac action potential. Alterations of its activation and inactivation properties due to mutations can cause severe, life-threatening arrhythmias. Yet despite intensive research efforts, many functional aspects this remain poorly understood. For instance, undergoes extensive post-translational modification in vivo , but significance these modifications is largely unexplored, especially under pathological conditions. This...
A rotating power transmission unit, namely gears experience contact stresses during meshing, all of which can be visualised in the teeth, is with another gear enabling transmission. This article presents an optimisation approach developed between contacts minimised by selecting epicyclic train having a specific set parameters, will discussed article. To conduct this experiment variation different control factors, Taguchi’s Design Experiment (DoE) methodology was considered, because it...
ABSTRACT Cannabis sativa contains active constituents called phytocannabinoids. Some phytocannabinoids are psychotropic and others not. The primary non-psychotropic phytocannabinoid is cannabidiol (CBD), which proposed to be therapeutic against many conditions, including muscle spasms. Mechanisms have been for the action of CBD on different systems, involving multiple targets, voltage-gated sodium channel (Nav) family, heavily implicated in conditions has reported relieve. In this study, we...
Abstract Voltage-gated sodium channels are heterotetrameric selective ion that play a central role in electrical signaling excitable cells. With recent advances structural biology, structures of eukaryotic have been captured several distinct conformations corresponding to different functional states. The secondary structure the pore lining S6 helices subunit DI, DII, and DIV has with both short π-helix stretches fully α-helical conformations. relevance these elements for gating is not yet...
veterinary usage, which ultimately created a historic impact on human health, showing improvement the nutritive factor, health status, and well-being of billions beings worldwide while subsequently, it was primarily used to treat onchocerciasis (river blindness) in humans during 1988. 4,5It has been proved suitable many ways such as highly effective, broad-spectrum, safe, well-tolerated, might be easily dispensed. 4Although for treatment variety internal nematode infections is well known...
Here we present the structural and electronic properties of free tin substituted copper Cun-mSnm (n=10, 13; m=0, 1, 2) clusters. The ground state have been calculated using plane wave based pseudopotential approach under spin-polarized density functional theory. results show that Cu13 cluster favors a stacked layer structure over spherically symmetric icosahedron structure. substitution Cu atoms by Sn changes relative stability pattern isomers. Moreover, favor to substitute surface than atom...
Abstract Voltage gated sodium channels play an important role in electrical signaling excitable cells. In response to changes membrane potential, they cycle between nonconducting and conducting conformations. With recent advances structural biology, structures of have been captured several distinct conformations, thought represent different functional states. However, it has difficult capture the intrinsically transient open state. We recently showed that a proposed state bacterial channel...
Abstract Voltage-gated sodium (Nav) channels play critical roles in propagating action potentials and otherwise manipulating ionic gradients excitable cells. These open response to membrane depolarization, selectively permeating ions until rapidly inactivating. Structural characterization of the gating cycle this channel family has proved challenging, particularly due transient nature state. A structure from bacterium Magnetococcus marinus Nav (NavMs) was initially proposed be open, based on...