A5025956141- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Marine Ecology and Invasive Species
- Protist diversity and phylogeny
- Diabetes and associated disorders
- Marine Biology and Ecology Research
- Neurotransmitter Receptor Influence on Behavior
- Environmental DNA in Biodiversity Studies
- vaccines and immunoinformatics approaches
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- Developmental Biology and Gene Regulation
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Marine Biology and Environmental Chemistry
- PARP inhibition in cancer therapy
- Urticaria and Related Conditions
- Mitochondrial Function and Pathology
- Marine and coastal plant biology
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
Okinawa Institute of Science and Technology Graduate University
2015-2024
Anhui Provincial Center for Disease Control and Prevention
2024
Washington State University
2024
TGS (United Kingdom)
2024
University of Pittsburgh
2019-2023
Stanford Health Care
2023
Stanford University
1992-2023
Children's Hospital Colorado
2020-2022
University of Colorado Denver
2020-2022
University of Arkansas at Fayetteville
2022
Genome structural variations within species are rare. How selective constraints preserve gene order and chromosome structure is a central question in evolutionary biology that remains unsolved. Our sequencing of several genomes the appendicularian tunicate Oikopleura dioica around globe reveals extreme genome scrambling caused by thousands chromosomal rearrangements, although showing no obvious morphological differences between these animals. The breakpoint accumulation rate an magnitude...
Antigen-specific T helper cells present in peripheral blood at very low frequencies are capable of rapid clonal expansion during antigenic challenge. The exquisite specificity this response provides for activation and a select cohort cells, feature we have used to directly identify quantify human epitope-specific from blood. Soluble tetramerized class II MHC molecules, loaded with an immunodominant peptide hemagglutinin (HA) labeled fluorescent dyes, were constructed antigen-specific...
Soluble HLA-DR401 or -DR404 tetramers containing a peptide corresponding to an immunodominant epitope from human GAD65 were used analyze peripheral blood T-cells of newly diagnosed type 1 diabetic patients and at-risk subjects. Peripheral mononuclear cells expanded on antigen-presenting presenting subsequently activated with specific plate-bound class II-peptide monomers. T-cell activation defined in flow cytometry by CD4(high) and/or CD25 markers observed all some subjects, but not normal...
The ability to change strategies in different contexts is a form of behavioral flexibility that crucial for adaptive behavior. striatum has been shown contribute certain forms such as reversal learning. Here we report on the contribution striatal cholinergic interneurons—a key element neuronal circuit—to strategy set-shifting which an attentional shift from one stimulus dimension another required. We made lesions rat interneurons dorsomedial or ventral using specific immunotoxin and...
Abstract T cell responses to Ags involve recognition of selected peptide epitopes contained within the antigenic protein. In this report, we describe a new approach for direct identification CD4+ complex that uses human class II tetramers identify reactive cells. With panel 60 overlapping peptides covering entire sequence VP16 protein, major Ag HSV-2, generated MHC loaded with pools were used stain peripheral lymphocytes an HSV-2 infected individual. approach, identified four...
Abstract Ag-specific CD4+ T cells are present in peripheral blood low frequency, where they undergo recruitment and expansion during immune responses the pathogenesis of numerous autoimmune diseases. MHC tetramers, which constitute a labeled MHC-peptide ligand suitable for binding to receptor on cells, provide novel approach detection characterization such rare cells. In this study, we utilized technology identify HLA DQ-restricted human subjects immunodominant epitopes associated with viral...
Abstract Sorafenib (SOR) is the first-line treatment for hepatocellular carcinoma (HCC). However, its use hindered by recently expressed safety concerns. One approach reducing SOR toxicity to lower doses in combination with other less toxic agents. Biochanin-A (Bio-A), a promising isoflavone, showed selective liver cancer cells. We postulated that combining and Bio-A could be synergistically towards HCC further evaluated underlying mechanism. Cytotoxicity assay was performed determine IC 50...
Abstract Behavioural flexibility is crucial for adaptive behaviour, and recent evidence suggests that cholinergic interneurons of the striatum play a distinct role. Previous studies function have focused on strategy switching by dorsomedial or ventral striatum. We here investigated whether in dorsolateral similar role at level habitual responses. Because particularly involved responding, we developed habit substitution task lever‐press responses to one side another. first measured effect...
Antigen-specific T cells acquire a distinctive phenotype during activation, with characteristic acquisition of surface markers and patterns gene expression. Early after antigen stimulation, CD4(+) lymphocytes increase their density the CD4 marker, trait which has been used to identify antigen-activated cells. The recent development MHC tetramer technologies greatly improved ability detect HLA class I-restricted specific for known epitopes. We have recently extended these studies human...
Abstract Background The larvacean Oikopleura dioica is an abundant tunicate plankton with the smallest (65–70 Mbp) non-parasitic, non-extremophile animal genome identified to date. Currently, there are two genomes available for Bergen (OdB3) and Osaka (OSKA2016) O. laboratory strains. Both assemblies have full coverage high sequence accuracy. However, a chromosome-scale assembly has not yet been achieved. Results Here, we present (OKI2018_I69) of Okinawan produced using long-read Nanopore...
The polyclonal nature of T cells expanding in an ongoing immune response results a range disparate affinities and activation potential. Recently developed human class II tetramers provide means to analyze this diversity by direct characterization the trimolecular TCR-peptide-MHC interaction live cells. Two HSV-2 VP16(369-379)-specific, DQA1*0102/DQB1*0602 (DQ0602)-restricted cell clones were compared proliferation assay HLA-DQ0602 tetramer staining. These two obtained from same subject, but...
Abstract HLA-DQ alleles are closely associated with susceptibility and resistance to insulin-dependent diabetes mellitus (IDDM) but the immunologic mechanisms involved not understood. Structural studies of IDDM-susceptible allele, HLA-DQA1*0301/DQB1*0302, have classified it as a relatively unstable dimer, particularly at neutral pH. This is reminiscent in nonobese diabetic mouse, which I-Ag7 unstable, contrast other murine I-A alleles, suggesting correlation between MHC class II molecules...
Behavioral flexibility is vital for survival in an environment of changing contingencies. The nucleus accumbens may play important role behavioral flexibility, representing learned stimulus–reward associations neural activity during response selection and learning from results. To investigate the we used light-activated halorhodopsin to inhibit shell neurons specific time segments a bar-pressing task requiring win–stay/lose–shift strategy. We found that optogenetic inhibition action segment...
Background: Automation has increasingly become more commonplace in the research laboratory workspace. The introduction of articulated robotic arms allows researcher flexibility tasks a single piece automated machinery can perform. We set out to incorporate automation processing genomic DNA organic extractions increase throughput and limit researchers exposure solvents. Methods: In order automate genome sequencing pipeline our laboratory, we programmed dual-arm anthropomorphic robot, Robotic...
Abstract Appendicularian tunicates are some of the most abundant mesozooplankton organisms with key roles in marine trophic webs and global carbon flux. Like appendicularians cosmopolitan distributions, Oikopleura dioica Fol, 1872 is considered a single species worldwide based on morphological features that distinguish them from other appendicularians. Despite their abundance, however, there still only ~ 70 described appendicularian species, compared to over 2800 ascidian tunicates. Here we...
Abstract Studies of the stability HLA-DQ have revealed a correlation between SDS MHC class II αβ dimers and insulin-dependent diabetes mellitus (IDDM) susceptibility. The dimer encoded by HLA-DQA1*0102/DQB1*0602 (DQ0602), which is dominant protective allele in IDDM, exhibits greatest among molecules EBV-transformed B-lymphoblastoid cells PBLs. DQ0602 also uniquely stable HLA-DM-deficient cell line, BLS-1. We addressed molecular mechanism A panel mutants based on polymorphic differences...