A5084892427- Neuroscience and Neuropharmacology Research
- Barrier Structure and Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Sensors and Ion Detection
- Ion channel regulation and function
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Neonatal and fetal brain pathology
- Mycobacterium research and diagnosis
- Advanced Neuroimaging Techniques and Applications
- Ion Transport and Channel Regulation
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood properties and coagulation
University of Pittsburgh
2015-2017
Increasing evidence has demonstrated a significant role of long non-coding RNAs (lncRNAs) in diverse biological processes. However, their functions cerebral ischemia remain largely unknown. Through an lncRNA array analysis rat model focal ischemia/reperfusion (I/R), we have identified CAMK2D-associated transcript 1 (C2dat1) as novel I/R-induced that regulated the expression CaMKIIδ murine models ischemia. C2dat1 mRNA was upregulated time-dependent manner mouse cortical penumbra after...
Abstract Stimulation of Na + /H exchanger isoform 1 (NHE1) in astrocytes causes ionic dysregulation under ischemic conditions. In this study, we created a Nhe1 flox/flox ( f/f ) mouse line with exon 5 flanked two loxP sites and selective ablation was achieved by crossing mice Gfap‐Cre ERT2 Cre‐recombinase mice. ERT2+/− ;Nhe1 at postnatal day 60–90 were treated either corn oil or tamoxifen (Tam, 75 mg/kg/day, i.p.) for days. After 30 days post‐injection, underwent transient middle cerebral...
The WNK-SPAK kinase signaling pathway controls renal NaCl reabsorption and systemic blood pressure by regulating ion transporters channels. A WNK3-SPAK complex is highly expressed in brain, but its function this organ remains unclear. Here, we investigated the role of brain edema white matter injury after ischemic stroke. Wild-type, WNK3 knockout, SPAK heterozygous or knockout mice underwent transient middle cerebral artery occlusion. One cohort magnetic resonance imaging. Ex-vivo brains...
γ-Aminobutyric acid (GABA) is a major neurotransmitter and plays important roles in both the developing mature central nervous system (CNS). One way that GABA can act by binding to fast, ionotropic GABAA receptors neurons. The of causes conformational change opens ion channels, allowing passage Cl– ions. In adults, intracellular concentration [Cl–]i) low, opening these channels triggers influx ions, causing hyperpolarization neuronal inhibition (Blaesse, 2009). However, system, [Cl–]i high,...
We previously reported that Na + /H exchanger isoform 1 (NHE1) plays a role in the dysregulation of ionic homeostasis astrocytes under ischemic conditions. In this study, we created an Nhe1 flox/flox mouse line ( f/f ) with exon 5 flanked two loxP sites order to selectively ablate NHE1 when crossed cell‐type specific GFAPCreER T2 Cre‐recombinase line. Gfap CreER/+ ;NHE1 mice at P60–90 were treated either tamoxifen (Tam, 75mg/kg/day, i.p.) or corn oil for days induce Cre recombination. 30...