A5032140542- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Inflammasome and immune disorders
- Neonatal Respiratory Health Research
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Glioma Diagnosis and Treatment
- Neurological Disease Mechanisms and Treatments
- Obstructive Sleep Apnea Research
- Erythrocyte Function and Pathophysiology
- Heavy Metal Exposure and Toxicity
- Cancer Immunotherapy and Biomarkers
- Autophagy in Disease and Therapy
- Complement system in diseases
- Caveolin-1 and cellular processes
- Nuclear Receptors and Signaling
- Hair Growth and Disorders
- PARP inhibition in cancer therapy
- Nanoparticles: synthesis and applications
- Genomics, phytochemicals, and oxidative stress
Polish Academy of Sciences
2013-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2021-2024
Mossakowski Medical Research Institute, Polish Academy of Sciences
2009-2021
Medical University of Warsaw
2018-2019
James Graham Brown Foundation
2019
University of Louisville
2019
Most mitochondrial proteins originate from the cytosol and require transport into organelle. Such precursor must be unfolded to pass through translocation channels in membranes. Misfolding of transported can result their arrest failure. Arrested block further import, disturbing functions cellular proteostasis. Cellular responses failure have been defined yeast. We developed cell line-based translocase clogging model discover molecular mechanisms that resolve failed import events humans. The...
α-Synuclein (ASN) plays an important role in pathogenesis of Parkinson's disease (PD) and other neurodegenerative disorders. Novel most interesting data showed elevated tauopathy PD suggested relationship between ASN Tau protein. However, the mechanism ASN-evoked protein modification is not fully elucidated. In this study we investigated extracellular hyperphosphorylation rat pheochromocytoma (PC12) cells involvement glycogen synthase kinase-3β (GSK-3β) cyclin-dependent kinase 5 (CDK5)...
Mobilization of stem cells from bone marrow (BM) into peripheral blood (PB) in response to tissue or organ injury, infections, strenuous exercise, mobilization-inducing drugs is as we postulated result a "sterile inflammation" the BM microenvironment that triggers activation Complement Cascade (ComC). Therefore, became interested role Nlrp3 inflammasome this process and show for first time its ATP-dependent manner orchestrates egress hematopoietic stem/progenitor (HSPCs) well other cells,...
α-Synuclein (ASN) and parkin, a multifunctional E3 ubiquitin ligase, are two proteins that associated with the pathophysiology of Parkinson's disease (PD). Excessive release ASN, its oligomerization, aggregation, deposition in cytoplasm contribute to neuronal injury cell death through oxidative-nitrosative stress induction, mitochondrial impairment, synaptic dysfunction. In contrast, overexpression parkin provides protection against cellular stresses prevents dopaminergic neural loss several...
Aberrant secretion and accumulation of α-synuclein (α-Syn) as well the loss parkin function are associated with pathogenesis Parkinson’s disease (PD). Our previous study suggested a functional interaction between those two proteins, showing that extracellular α-Syn evoked post-translational modifications parkin, leading to its autoubiquitination degradation. While plays an important role in mitochondrial biogenesis turnover, including fission/fusion mitophagy, involvement deregulation...
Parkin and alpha-synuclein (α-syn) are two key proteins involved in the pathophysiology of Parkinson's disease (PD). Oligomerization/aggregation excessive secretion α-syn contributes to PD through free radical stress, mitochondrial impairment, synaptic dysfunction. Parkin, an E3 ubiquitin ligase, is considered be a pleiotropic, neuroprotective protein that modulates metabolic turnover accumulation α-syn. This addition parkin's role counteracting more distant effects on cellular survival by...
The complement cascade (ComC) cleavage fragments C3a and C5a regulate the trafficking of normal, differentiated hematopoietic cells, although they do not chemoattract more primitive stem/progenitor cells (HSPCs). By contrast, human myeloid lymphoid leukemia cell lines clonogenic blasts from chronic myelogenous (CML) acute (AML) patients respond to C3 C5 by chemotaxis increased adhesion. Consistent with this finding, receptors are expressed leukemic at mRNA (RT-PCR) protein (FACS) levels,...
Imbalance between production and scavenging of free radicals other reactive oxygen species (ROS) is a component many diseases, but it especially important in aging-related diseases the central nervous system. Oxidative stress-induced neuronal dysfunction plays an role pathomechanism neurodegenerative disorders, including Alzheimer's Parkinson's disease. Experimental data showed that radical scavengers may protect brain against oxidative modifications. The need for efficient safe antioxidants...
Abstract Most mitochondrial proteins originate from the cytosol and require active transport into organelle. Such precursor must be largely unfolded to pass through translocation channels in membranes. Misfolding of transported can result their arrest failure. Arrested block further import, disturbing functions cellular proteostasis. Cellular responses failure have been defined yeast. To discover molecular mechanisms that resolve failed import events human cells, we developed translocase...
ABSTRACT Microglia (MG) are myeloid cells of the central nervous system supporting its homeostasis and instigating neuroinflammation in pathologies. Single-cell RNA sequencing (scRNA-seq) revealed functional heterogeneity MG mice brains. Inhibition colony-stimulating factor 1 receptor (CSF1R) signaling with inhibitors deplete microglia which rapidly repopulate. Functionalities repopulated poorly known. We combined scRNA-seq, bulk RNA-seq, immunofluorescence confocal imaging to study...
Abstract BACKGROUND Glioblastoma (GBM) is a highly diffusive tumor which restricts the efficacy of surgical resection and facilitates recurrence. Matrix metalloproteinase 2 (MMP2) extracellular matrix protease that activated by MMP14 expressed microglia plays central role in tissue remodeling progression. The R132H mutation isocitrate dehydrogenase 1 (IDH1R132H) commonly observed gliomas, results hypermethylator phenotype GBMs associated with better survival. impacts IDH1R132H on...
Abstract Clinical trials with immune checkpoint inhibitors (ICI) have benefited many cancer patients but failed in a number of tumors, including glioblastoma (GBM), the most common and aggressive primary brain tumor adults. The main obstacle for ICI efficacy GBM is continuously evolving microenvironment (TME), which antitumor immunity inhibited or eluded by tumor-secreted factors. Accumulation reprogramming myeloid cells (GAMs) creates “cold” immunosuppressive TME poor infiltration...