A5033774475- Telomeres, Telomerase, and Senescence
- Chromosomal and Genetic Variations
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Polar Research and Ecology
- Genomics and Phylogenetic Studies
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Circadian rhythm and melatonin
- Bee Products Chemical Analysis
- Plant Virus Research Studies
- Protist diversity and phylogeny
- Environmental DNA in Biodiversity Studies
- Genomics, phytochemicals, and oxidative stress
- Genomics and Chromatin Dynamics
- Microplastics and Plastic Pollution
- Neurobiology and Insect Physiology Research
- Entomopathogenic Microorganisms in Pest Control
- bioluminescence and chemiluminescence research
- Glutathione Transferases and Polymorphisms
- Paraquat toxicity studies and treatments
- RNA and protein synthesis mechanisms
Czech Academy of Sciences, Biology Centre
2013-2024
Institute of Entomology
2013-2024
University of South Bohemia in České Budějovice
2017-2024
Czech Academy of Sciences
2019-2023
National Institute of Environmental Health Sciences
2007-2022
Laboratory of Molecular Genetics
2008
National Institutes of Health
2007
Changes in genome architecture often have a significant effect on ecological specialization and speciation. This may be further enhanced by involvement of sex chromosomes playing disproportionate role reproductive isolation. We physically mapped the Z chromosome major pome fruit pest, codling moth, Cydia pomonella (Tortricidae), show that it arose fusion between an ancestral autosome corresponding to 15 Bombyx mori reference genome. originated common ancestor main tortricid subfamilies,...
We examined the presence of TTAGG telomeric repeats in 22 species from 20 insect orders with no or inconclusive information on telomere composition by single-primer polymerase chain reaction (TTAGG) 6 primers, Southern hybridization genomic DNAs, and fluorescence situ chromosomes n probes. The sequence was present 15 absent 7 species. In a compilation new published data, we combined distribution motif phylogenetic tree. pattern clearly supported hypothesis that an ancestral telomeres but...
Abstract This paper reports results of the first cytogenetic investigation carried out in carob moth, Ectomyelois ceratoniae Zeller, serious polyphagous pest both stored products and field crops Mediterranean basin Near East regions. Preparations mitotically dividing spermatogonia oogonia revealed that moth karyotype consists 2n = 62 chromosomes. As typical for Lepidoptera, chromosomes were a holokinetic type, only slightly differed their sizes, displayed no morphological structures...
Despite a high toxicity, paraquat is one of the most widely used herbicides in world. Our study evaluated effect exposure on antioxidant response and locomotion activity Drosophila melanogaster. We examined enzymatic superoxide dismutase (SOD) catalase, transcript levels both enzymes. Flies were exposed to wide range concentrations (0.25 μM 25 mM) for 12 h. SOD, at levels, revealed biphasic dose–response curve with peak 2.5 paraquat. A similar was observed catalase. Males higher...
Abstract Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption their nucleoprotein composition usually results in genome instability cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved evolution, most common pattern telomeric across eukaryotes is (T x A y G z ) n maintained by telomerase. However, telomerase-added repeats some insect taxa frequently vary, show unusual features, can even absent. It has speculated...
Abstract In contrast to the catalytic subunit of telomerase, its RNA (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin TRs transcribed with polymerase II Opisthokonta (the supergroup including Animalia Fungi) Trypanosomida one hand, III under control type 3 promoter, found TSAR Archaeplastida supergroups (including e.g. ciliates Viridiplantae taxa, respectively). Here, we focus unknown largest order -...
Abstract Three widely accepted assumptions are based on telomere research in human cells: (i) length is a determinant of replicative ageing; (ii) telomerase activity somatic cells supports the proliferative capacity and thus contributes to their regenerative potential organismal lifespan; (iii) lack acts as tumour suppression mechanism. However, from broader view, link between biology cellular ageing, well development, remains debate, I demonstrate with numerous examples invertebrate...
Kings and queens of termites, like other advanced eusocial insects, are endowed with admirable longevity, which dramatically exceeds the life expectancies their non-reproducing nest-mates related solitary insects. In quest to find mechanisms underlying longevity termite reproductives, we focused on somatic maintenance mediated by telomerase. This ribonucleoprotein is well established for pro-longevity functions in vertebrates, thanks primarily its ability telomere extension. However,...
This study examined the expression and role of vitellogenin (Vg) in body firebug Pyrrhocoris apterus (Heteroptera, Insecta) during infection elicited by two entomopathogenic organisms, nematode Steinernema carpocapsae fungus Isaria fumosorosea. Infection S. significantly up-regulated Vg mRNA male body. The corresponding increase protein was also confirmed electrophoretic immunoblotting analyses. Remarkably, females, tendency opposite. Nematodal reduced both levels fat hemolymph,...
In honeybees (Apis mellifera), the rate of aging is modulated through social interactions and according to caste differentiation seasonal (winter/summer) generation workers. Winter workers, which hatch at end summer, have remarkably extended lifespans as an adaptation cold season when resources required for growth reproduction colonies are limited bees need maintain colony until next spring. contrast, summer only live several weeks. To better understand lifespan differences between winter...
Abstract Telomeric regions in Drosophila are composed of three subdomains. A chromosome cap distinguishes the end from a DNA double-strand break; an array retrotransposons, HeT-A, TART, and TAHRE (HTT), maintains telomere length by targeted transposition to ends; telomere-associated sequence (TAS), which consists mosaic complex repeated sequences, has been identified as source gene silencing. Heterochromatin protein 1 (HP1) HP1-ORC-associated (HOAP) major components required for stability....
Insect vitellogenins are an intriguing class of complex proteins. They primarily serve as a source energy for the developing embryo in insect eggs. Vitellogenesis is hormonally and neurally controlled process that command synthesis vitellogenin molecules ensures their transport from female fat bodies or ovarial cells into The representatives all hormones such juvenile hormones, ecdysteroids, neurohormones participate vitellogenesis, but (most species) ecdysteroids (mostly Diptera) play most...
Drosophila melanogaster telomeres have two DNA domains: a terminal array of retrotransposons and subterminal repetitive telomere-associated sequence (TAS), source telomere position effect (TPE). We reported previously that deletion the 2L TAS leads to dominant suppression TPE by stimulating in trans expression telomeric transgene. Here, we compared transcript activities w transgene inserted between retrotransposon arrays at genotypes with different lengths TAS. In contrast individuals...