A5035772207- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Virus-based gene therapy research
- Proteoglycans and glycosaminoglycans research
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Carbohydrate Chemistry and Synthesis
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- SARS-CoV-2 and COVID-19 Research
- Natural product bioactivities and synthesis
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Biological Activity of Diterpenoids and Biflavonoids
- Animal Disease Management and Epidemiology
- Traditional and Medicinal Uses of Annonaceae
- Neonatal Respiratory Health Research
- T-cell and Retrovirus Studies
- Phytochemistry and Biological Activities
- Lung Cancer Research Studies
- Mosquito-borne diseases and control
University of Gothenburg
2015-2025
Institute for Biomedicine
2020
Infectious Diseases Institute
2020
The University of Queensland
2005
Australian National University
2005
University of Minnesota
1993
University of Computer Sciences and Economics in Olsztyn
1987-1993
SARS-CoV-2 was discovered among humans in Wuhan, China late 2019, and then spread rapidly, causing a global pandemic. The virus found to be transmitted mainly by respiratory droplets from infected persons or direct contact. It also shown excreted feces, why we investigated whether the could detected wastewater if so, which extent its levels reflects society. Samples of city Gothenburg, surrounding municipalities Sweden were collected daily mid-February until June 2020 at Rya treatment plant....
Heparan sulfate (HS) is a cell surface polysaccharide recently identified as coreceptor with the ACE2 protein for S1 spike on SARS-CoV-2 virus, providing tractable new therapeutic target. Clinically used heparins demonstrate an inhibitory activity but have anticoagulant and are supply-limited, necessitating alternative solutions. Here, we show that synthetic HS mimetic pixatimod (PG545), cancer drug candidate, binds destabilizes receptor binding domain directly inhibits its to ACE2,...
Coronaviruses raise serious concerns as emerging zoonotic viruses without specific antiviral drugs available. Here we screened a collection of 16671 diverse compounds for anti-human coronavirus 229E activity and identified an inhibitor, designated K22, that specifically targets membrane-bound coronaviral RNA synthesis. K22 exerts most potent after virus entry during early step the viral life cycle. Specifically, formation double membrane vesicles (DMVs), hallmark replication, was greatly...
Cell surface heparan sulfates mediate primary attachment of herpes simplex virus type 1, the first step in invasion cells. Removal host cell sulfate results a significantly diminished susceptibility to infection. On envelope, glycoprotein C has been identified as major binding site for Using selectively desulfated heparins and metabolically labeled sulfate, we have analyzed structural requirements provide sites whole virus. Employing affinity chromatography assay, subfractionated...
ABSTRACT Cell surface heparan sulfate (HS) serves as an initial receptor for many different viruses, including herpes simplex virus types 1 and 2 (HSV-1 2, respectively). Glycoproteins C B (gC gB) are the major components of viral envelope that mediate binding to HS. In this study, purified gB gC homologous proteins well HSV-1 HSV-2 virions were compared ability bind isolated HS molecules. bound comparable amounts Similarly, its counterpart showed no difference in HS-binding capabilities....
The synthetic n-alkyl esters of gallic acid, also known as gallates, are widely employed antioxidants by food and pharmaceutical industries. Besides the antioxidant activity, other biological activities have been described for this group molecules, mainly anticancer, antibacterial antifungal properties. In present study, anti-herpes simplex virus (HSV)-2 activity acid pentyl gallate was evaluated followed determination site antiviral these compounds. Our results demonstrated that both...
Although cell surface chondroitin sulfate (CS) is regarded as an auxiliary receptor for binding of herpes simplex virus to cells, and purified CS chain types A, B, C are known interfere poorly or not at all with the infection we have found that type E (CS-E), derived from squid cartilage, exhibited potent antiviral activity. The IC(50) values ranged 0.06 0.2 mug/ml substantially exceeded potency heparin, inhibitor cells. Furthermore, in mutant gro2C cells express but heparan sulfate, CS-E...
The synthetic n-alkyl esters of gallic acid (GA), also known as gallates, especially propyl, octyl and dodecyl are widely employed antioxidants by food pharmaceutical industries. inhibitory effects GA 15 gallates on Herpes Simplex Virus type 1 (HSV-1) Human Immunodeficiency (HIV-1) replication were investigated here. After a preliminary screening these compounds, pentyl gallate (PG) seemed to be the most active compounds against HSV-1 their mode action was characterized through set assays,...
The amino acid residues critical for interaction between herpes simplex virus type 1 (HSV-1) glycoprotein C (gC- 1) and cell surface heparan sulphate (HS) were localized to two separate regions within antigenic site II of this glycoprotein. These acids Arg-143, Arg-145, Arg-147 Thr-150 in one region Gly-247 the other. This conclusion is based on following observations, (i) Monoclonal antibodies defining gC-1 II, not those reactive with I, inhibited HSV-1-induced haemagglutination binding...
We have demonstrated a defect in expression of chondroitin 4-O-sulfotransferase-1 (C4ST-1) murine sog9 cells, which are poorly sensitive to infection by herpes simplex virus type 1 (HSV-1). Sog9 cells were previously isolated as CS-deficient from gro2C partially resistant HSV-1 and defective the heparan sulfate (HS) because splice site mutation EXT1 gene encoding HS-synthesizing enzyme. Here we detected small amount CS chains with drastic decrease 4-O-sulfation compared parental cells....
Background Tick-borne encephalitis (TBE) is a neurological disease caused by the tick-borne virus (TBEV). Despite available vaccines, breakthrough infections occur, some fatal.
The phosphosulfomannan 1 (PI-88) is a mixture of highly sulfated oligosaccharides that currently undergoing clinical evaluation in cancer patients. As well as its anticancer properties, displays number other interesting biological activities. A series analogues were synthesized with single carbon (pentasaccharide) backbone to facilitate structural characterization and interpretation results. In fashion similar 1, all compounds able inhibit heparanase bind tightly the proangiogenic growth...
The role of glycoprotein C (gC) for binding herpes simplex virus type 1 (HSV-1) to cell surface chondroitin sulfate (CS) and the consequences this interaction attachment infectivity were studied. To end, a panel HSV-1 gC mutants, including gC-negative (gC − ) variant, mouse fibroblasts expressing either CS or heparan (HS) used. Comparing gC-positive + viruses in terms their infection CS-expressing cells indicated that was essential both functions. Furthermore, purified bound efficiently...
Glycoprotein C (gC) mediates the attachment of HSV-1 to susceptible host cells by interacting with glycosaminoglycans (GAGs) on cell surface. gC contains a mucin-like region located near GAG-binding site, which may affect binding activity. Here, we address this issue studying mutant lacking domain in and corresponding purified protein (gCΔmuc) culture assays, respectively. The virus exhibited two functional alterations as compared native (i.e. decreased sensitivity GAG-based inhibitors...
Heparan sulfate (HS) has been identified as a receptor molecule for numerous microbial pathogens, including herpes simplex virus type 1 (HSV-1). To further define the major HS-binding domain of HSV-1 attachment protein, i.e. glycoprotein C (gC), mutants carrying alterations either two neighbouring basic amino acid residues or single hydrophobic residue within N-terminal protein (residues 26–227) were constructed. In addition, mutant lacking Asn148 glycosylation site was included in study....
Discovery and development of new antiviral therapies essentially rely on two key factors: an in-depth understanding the mechanisms involved in viral infection fast versatile drug screening platforms. To meet those demands, we present a biosensing platform to probe virus–cell membrane interactions single particle level. Our method is based formation supported lipid bilayers from cell material. Using total internal reflection fluorescence microscopy, report contribution cellular components...
Glycoprotein G is a major target for the humoral immune response against herpes simplex virus (HSV) and prototype antigen type-specific serodiagnosis discriminating HSV-1 HSV-2 infections. The mature part of glycoprotein G-2 (gG-2) contains unique stretch suspected to mediate type specificity, in addition region homologous G-1 (gG-1). Antigenic determinants gG-2 were mapped by testing reactivity mouse anti-gG-2 monoclonal antibodies (MAbs) purified human with synthetic peptides coupled...
Herpes simplex virus (HSV) and many other viruses, including HIV, initiate infection of host cells by binding to glycosaminoglycan (GAG) chains cell surface proteoglycans. Although GAG mimetics, such as sulfated oligo- polysaccharides, exhibit potent antiviral activities in cultured cells, the prophylactic application these inhibitors vaginal microbicides failed protect women upon their exposure HIV. A possible explanation for this failure is that polysaccharides no typical virucidal...