A5080335939- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Protein Structure and Dynamics
- Microbial Fuel Cells and Bioremediation
- SARS-CoV-2 detection and testing
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Cancer Research and Treatments
- Cerebrospinal fluid and hydrocephalus
- Global Health and Surgery
- Polyamine Metabolism and Applications
- Marine and coastal ecosystems
- CO2 Reduction Techniques and Catalysts
- Genetics, Bioinformatics, and Biomedical Research
- ATP Synthase and ATPases Research
- Protist diversity and phylogeny
- T-cell and B-cell Immunology
- Biomedical and Engineering Education
- Amino Acid Enzymes and Metabolism
- Hemoglobin structure and function
- Bacterial Genetics and Biotechnology
- CAR-T cell therapy research
University of California, Berkeley
2018-2024
Innovative Genomics Institute
2020-2022
University of California, San Francisco
2021-2022
Immunotargeting of tumor-specific antigens is a powerful therapeutic strategy. Immunotherapies directed at MHC-I complexes have expanded the scope and enabled direct targeting intracellular oncoproteins cell surface. We asked whether covalent drugs that alkylate mutated residues on could act as haptens to generate unique MHC-I-restricted neoantigens. Here, we report KRAS G12C mutant cells treated with inhibitor ARS1620 present ARS1620-modified peptides in complexes. Using ARS1620-specific...
Many photosynthetic organisms employ a CO 2 concentrating mechanism (CCM) to increase the rate of fixation via Calvin cycle. CCMs catalyze ≈50% global photosynthesis, yet it remains unclear which genes and proteins are required produce this complex adaptation. We describe construction functional CCM in non-native host, achieved by expressing from an autotrophic bacterium Escherichia coli strain engineered depend on rubisco carboxylation for growth. Expression 20 enabled E. grow fixing...
Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO 2 fixation—Rubisco and carbonic anhydrase—and the centerpiece bacterial concentrating mechanism (CCM). In CCM, actively accumulated cytosolic bicarbonate diffuses into carboxysome is converted to by anhydrase, producing a high concentration near Rubisco ensuring efficient carboxylation. Self-assembly α-carboxysome orchestrated intrinsically disordered scaffolding protein, CsoS2, which interacts with both...
Despite its prominence, the ability to engineer Cupriavidus necator H16 for inorganic carbon uptake and fixation is underexplored. We tested roles of endogenous heterologous genes on C. metabolism. Deletion β-carbonic anhydrase can had most deleterious effect autotrophic growth. Replacement this native system with several classes dissolved (DIC) transporters from Cyanobacteria chemolithoautotrophic bacteria recovered growth supported higher cell densities compared wild-type (WT) in batch...
Cyanobacteria rely on CO 2 -concentrating mechanisms (CCMs) to grow in today’s atmosphere (0.04% ). These complex physiological adaptations require ≈15 genes produce two types of protein complexes: inorganic carbon (Ci) transporters and 100+ nm carboxysome compartments that encapsulate rubisco with a carbonic anhydrase (CA) enzyme. Mutations disrupting any these prohibit growth ambient air. If plausible ancestral form—i.e., lacking single gene—cannot grow, how did the CCM evolve? Here, we...
Summary Rapid nucleic acid testing is a critical component of robust infrastructure for increased disease surveillance. Here, we report microfluidic platform point-of-care, CRISPR-based molecular diagnostics. We first developed test which pairs distinct mechanisms DNA and RNA amplification optimized high sensitivity rapid kinetics, linked to Cas13 detection specificity. combined this workflow with an extraction-free sample lysis protocol using shelf-stable reagents that are widely available...
ABSTRACT Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO 2 fixation - Rubisco and carbonic anhydrase the centerpiece bacterial concentrating mechanism (CCM). In CCM, actively accumulated cytosolic bicarbonate diffuses into carboxysome is converted to by anhydrase, producing a high concentration near ensuring efficient carboxylation. Self-assembly α-carboxysome orchestrated intrinsically disordered scaffolding protein, CsoS2, which interacts with both carboxysomal...
Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided recognition that triggers cleavage and release a fluorescent reporter molecule
Abstract Uptake and fixation of CO 2 are central to strategies for -based biomanufacturing. Cupriavidus necator H16 has emerged as a promising industrial host this purpose. Despite its prominence, the ability engineer C. inorganic carbon uptake is underexplored. Here, we test role endogenous heterologous genes on metabolism. Deletion one four carbonic anhydrases in , β-carbonic anhydrase can had most deleterious effect autotrophic growth. Replacement native system with several classes...
Science education and research have the potential to drive profound change in low- middle-income countries (LMICs) through encouraging innovation, attracting industry, creating job opportunities. However, LMICs, capacity is often limited, acquisition of funding access state-of-the-art technologies challenging. The Alliance for Global Health (the Alliance) was founded as a partnership between University California, Berkeley (USA) Makerere (Uganda), with goal strengthening University's...
Abstract Many bacterial autotrophs rely on CO 2 concentrating mechanisms (CCMs) to assimilate carbon. Although many CCM proteins have been identified, including a 200+ MDa protein organelle called the carboxysome, systematic screen of components has not carried out. Here, we performed genome-wide barcoded transposon identify essential and CCM-related genes in ɣ-proteobacterium H. neapolitanus . Our revealed an operon critical for function which encodes domain unknown (PFAM:PF10070) putative...
Abstract Many photosynthetic organisms employ a CO 2 concentrating mechanism (CCM) to increase the rate of fixation via Calvin cycle. CCMs catalyze ≈50% global photosynthesis, yet it remains unclear which genes and proteins are required produce this complex adaptation. We describe construction functional CCM in non-native host, achieved by expressing from an autotrophic bacterium engineered E. coli strain. Expression 20 enabled grow fixing ambient air into biomass, with growth depending on...
Abstract Cyanobacteria rely on CO 2 concentrating mechanisms (CCMs) that depend ≈15 genes to produce two protein complexes: an inorganic carbon (Ci) transporter and a 100+ nm carboxysome compartment encapsulates rubisco with carbonic anhydrase (CA) enzyme. Mutations disrupting CCM components prohibit growth in today’s atmosphere (0.04% ), indicating CCMs evolved cope declining environmental . Indeed, geochemical data models indicate atmospheric has been generally decreasing from high...