A5009888142- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Parasites and Host Interactions
- 3D Printing in Biomedical Research
- Advanced Materials and Mechanics
- Electrowetting and Microfluidic Technologies
- Global Maternal and Child Health
- Global Health and Surgery
- Micro and Nano Robotics
- Microfluidic and Capillary Electrophoresis Applications
- Parasite Biology and Host Interactions
- Electrohydrodynamics and Fluid Dynamics
- Viral Infections and Immunology Research
- Zoonotic diseases and public health
- Microfluidic and Bio-sensing Technologies
- Photoacoustic and Ultrasonic Imaging
University of California, Berkeley
2020-2025
University of California, San Francisco
2020-2021
Tecnológico de Monterrey
2016-2020
University of British Columbia
2016
Third Way
2016
Kelowna General Hospital
2016
Abstract This paper introduces the concept of continuous chaotic printing, i.e. use flows for deterministic and extrusion fibers with internal multilayered micro- or nanostructures. Two free-flowing materials are coextruded through a printhead containing miniaturized Kenics static mixer (KSM) composed multiple helicoidal elements. produces fiber well-defined multilayer microarchitecture at high-throughput (>1.0 m min −1 ). The number mixing elements diameter determine thickness lamellae,...
In this article, we present a method for cell patterning and culture within hydrogel droplet on digital microfluidic (DMF) platform.
This paper presents an electrohydrodynamic technique for rapid mixing of droplets in open and closed digital microfluidic (DMF) platforms. Mixing is performed by applying a high frequency AC voltage to the coplanar or parallel electrodes, inducing circulation zones inside droplet which results content. The advantages proposed method comparison conventional methods that operate based on transporting back forth side include 1) shorter time (as fast as 0.25 s), 2) use fewer number reducing size...
Abstract Schistosomiasis currently affects over 250 million people and remains a public health burden despite ongoing global control efforts. Conventional microscopy is practical tool for diagnosis screening of Schistosoma haematobium , but identification eggs requires skilled microscopist. Here we present machine learning (ML)-based strategy automated detection S. that combines two imaging contrasts, brightfield (BF) darkfield (DF), to improve diagnostic performance. We collected BF DF...
Accurate diagnostics are essential for disease control and elimination efforts. However, access to neglected tropical diseases (NTDs) is hindered by limited healthcare infrastructure in many NTD-endemic regions, as well reliance on time- labor-intensive diagnostic methods, such smear microscopy. New tools that portable, rapid, low-cost, meet World Health Organization (WHO) sensitivity specificity targets urgently needed accelerate NTD programs. Here, we introduce the NTDscope, a portable...
SUMMARY The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge identifying symptomatic, asymptomatic and pre-symptomatic carriers virus. CRISPR-based diagnostics that utilize RNA DNA-targeting enzymes can augment gold-standard PCR-based testing if they be made rapid, portable accurate. Here we report development amplification-free CRISPR-Cas13a-based mobile phone assay for direct detection SARS-CoV-2 from nasal...
Schistosomiasis infections continue to impact African settings disproportionately, and there is an urgent need for novel tools evaluate infection control elimination strategies at the community level. Mobile phone microscopes are portable semiautomated devices with multiple applications screening neglected tropical diseases. In a community-based schistosomiasis program in Azaguié, Côte d'Ivoire, mobile microscopy demonstrated sensitivity of 85.7% (95% CI: 69.7-95.2%) specificity 93.3%...
Summary Rapid nucleic acid testing is a critical component of robust infrastructure for increased disease surveillance. Here, we report microfluidic platform point-of-care, CRISPR-based molecular diagnostics. We first developed test which pairs distinct mechanisms DNA and RNA amplification optimized high sensitivity rapid kinetics, linked to Cas13 detection specificity. combined this workflow with an extraction-free sample lysis protocol using shelf-stable reagents that are widely available...
Novel methods are required to aid the monitoring of schistosomiasis control and elimination initiatives through mass drug administration. Portable digital mobile phone microscopy is a promising tool for this purpose. This cross-sectional study evaluated diagnostic operating characteristics converted microscope (the SchistoScope) detection Schistosoma haematobium eggs, as determined by community-based field workers expert microscopists, compared with gold standard light microscopy. Three...
Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided recognition that triggers cleavage and release a fluorescent reporter molecule
Abstract This paper introduces the concept of continuous chaotic printing, i.e., use flows for deterministic and fabrication fibers with internal multilayered micro-or nanostructures. Two free-flowing materials are coextruded through a printhead containing miniaturized Kenics static mixer (KSM) composed multiple helicoidal elements. produces fiber well-defined multilayer microarchitecture at high speeds (>1.0 m min-1). The number mixing elements diameter determine thickness lamellae,...