A5088183580- Gut microbiota and health
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Extracellular vesicles in disease
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Biomedical and Engineering Education
- Genetics, Bioinformatics, and Biomedical Research
- Plant Virus Research Studies
- Global Health and Surgery
Stanford University
2018-2023
University of California, San Francisco
2022
Clostridioides difficile is a leading cause of infectious diarrhea in humans, and it imposes tremendous burden on the health care system. Current treatments for C. infection (CDI) include antibiotics fecal microbiota transplant, which contribute to recurrent CDIs face major regulatory hurdles, respectively.
LC3-dependent EV loading and secretion (LDELS) is a secretory autophagy pathway in which the macroautophagy/autophagy machinery facilitates packaging of cytosolic cargos, such as RNA-binding proteins, into extracellular vesicles (EVs) for outside cell. Here, we identify TFRC (transferrin receptor), one first proteins found to be secreted via EVs, transmembrane cargo LDELS pathway. Similar other targets, EVs genetically requires components MAP1LC3/LC3-conjugation but independent ATGs involved...
Abstract A variety of cell surface structures, including capsular polysaccharides (CPS), dictate interactions between bacteria and their environment viruses (bacteriophages). Members the prominent human gut Bacteroidetes characteristically produce several phase-variable CPS, but contributions to bacteriophage are unknown. We used engineered strains symbiont Bacteroides thetaiotaomicron , which differ only in CPS they express, isolate bacteriophages from two locations United States. Testing...
ABSTRACT A disrupted “dysbiotic” gut microbiome engenders susceptibility to the diarrheal pathogen Clostridioides difficile by impacting metabolic milieu of gut. Diet, in particular microbiota accessible carbohydrates (MACs) found dietary fiber, is one most powerful ways affect composition and output microbiome. As such, diet a tool for understanding biology C. developing alternative approaches coping with this pathogen. One prominent class metabolites produced are short chain fatty acids...
Science education and research have the potential to drive profound change in low- middle-income countries (LMICs) through encouraging innovation, attracting industry, creating job opportunities. However, LMICs, capacity is often limited, acquisition of funding access state-of-the-art technologies challenging. The Alliance for Global Health (the Alliance) was founded as a partnership between University California, Berkeley (USA) Makerere (Uganda), with goal strengthening University's...
Clostridium difficile (Cd) is an opportunistic diarrheal pathogen and Cd infection (CDI) represents a major healthcare concern, causing estimated 15,000 deaths per year in the United States alone 1 .Several enteric pathogens, including Cd, leverage inflammation accompanying microbial dysbiosis to thrive distal gut 2 .Although diet among most powerful available tools for affecting health of humans their relationship with microbiota, investigation into effects on CDI has been limited.Here, we...