A5055328910- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Vectors
- Immune cells in cancer
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Axon Guidance and Neuronal Signaling
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- Adipose Tissue and Metabolism
- Adenosine and Purinergic Signaling
- Apelin-related biomedical research
- Neurological diseases and metabolism
- Viral-associated cancers and disorders
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Immune Response and Inflammation
- Virus-based gene therapy research
- Prion Diseases and Protein Misfolding
Colorado State University
2020-2024
Loyola University Chicago
2023
Wayne State University
2023
Rush University Medical Center
2023
University of Colorado Anschutz Medical Campus
2011-2022
University of Colorado Denver
2011-2020
Color (United States)
2017
Recent studies have demonstrated dramatic shifts in metabolic supply-and-demand ratios during inflammation, a process resulting localized tissue hypoxia within inflammatory lesions (“inflammatory hypoxia”). As part of the adaptive immune response, T cells are recruited to sites hypoxia. Given profound effects on gene regulation, we hypothesized that T-cell differentiation is controlled by To pursue this hypothesis, analyzed transcriptional consequences ambient (1% oxygen) broad panel...
ABSTRACT Human papillomaviruses (HPVs) are small DNA viruses causally associated with benign warts and multiple cancers, including cervical head-and-neck cancers. While the vast majority of people exposed to HPV, most instances infection cleared naturally. However, intrinsic host defense mechanisms that block early establishment HPV infections remain mysterious. Several antiviral cytidine deaminases human APOBEC3 (hA3) family have been identified as potent viral mutators. editing genomes in...
<h3>Background</h3> Inflammatory bowel diseases, encompassing Crohn9s disease and ulcerative colitis, are characterised by persistent leucocyte tissue infiltration leading to perpetuation of an inappropriate inflammatory cascade. The neuronal guidance molecule netrin-1 has recently been implicated in the orchestration trafficking during acute inflammation. We therefore hypothesised that could modulate activity a model disease. <h3>Design</h3> DSS-colitis was performed mice with partial...
Acute lung injury (ALI) is characterized by alveolar and uncontrolled inflammation. Since most cases of ALI resolve spontaneously, understanding the endogenous mechanisms that promote resolution important to developing effective therapies. Previous studies have implicated extracellular adenosine signaling in tissue adaptation wound healing. Therefore, we hypothesized a functional contribution for production during resolution. As model, administered intratracheal LPS observed peak at 3 d,...
High-risk human papillomaviruses (HPVs) are causally associated with multiple cancers. Previous studies have shown that the HPV oncoprotein E7 induces immune suppression; however, underlying mechanisms remain unknown. To understand by which deregulates host responses in tumor microenvironment, we analyzed gene expression changes of all known chemokines and their receptors using our global data sets from HPV-positive -negative head/neck cancer cervical tissue specimens different disease...
Anti-inflammatory signals play an essential role in constraining the magnitude of inflammatory response. Extracellular adenosine is a critical tissue-protective factor, limiting extent inflammation. Given potent anti-inflammatory effects extracellular adenosine, we sought to investigate how regulates T cell activation and differentiation. Adenosine receptor by pan adenosine-receptor agonist enhanced abundance murine regulatory cells (Tregs), type Gene expression studies both naïve CD4 Tregs...
ABSTRACT APOBEC3 (A3) mutation signatures have been observed in a variety of human cancer genomes, including those cervical and head neck cancers caused by papillomavirus (HPV) infection. However, the driving forces that promote off-target A3 activity remain mostly unclear. Here, we report mechanism for dramatic increase A3A protein levels HPV-positive keratinocytes. We show expression viral E7 from high-risk HPVs, but not low-risk significantly prolongs cellular half-life keratinocytes cell...
Abstract Human papillomavirus (HPV) infection distinctly alters methylation patterns in HPV-associated cancer. We have recently reported that HPV E7-dependent promoter hypermethylation leads to downregulation of the chemokine CXCL14 and suppression antitumor immune responses. To investigate extent gene expression dysregulated by E7-induced DNA methylation, we analyzed parallel global using normal immortalized keratinocyte lines, NIKS, NIKS-16, NIKS-18, NIKS-16∆E7. show MHC class I genes is...
Evasion of the host immune responses is critical for both persistent human papillomavirus (HPV) infection and associated cancer progression. We have previously shown that expression homeostatic chemokine CXCL14 significantly downregulated by HPV oncoprotein E7 during Restoration in HPV-positive head neck (HNC) cells dramatically suppresses tumor growth increases survival through an immune-dependent mechanism mice. Although recruits natural killer (NK) T to microenvironment, which mediates...
Chronic wasting disease (CWD) affects cervids in North America, Asia, and Scandinavia. CWD is unique its efficient spread, partially because of contact with infectious prions shed secreta. To assess temporal profiles prion shedding, we collected saliva, urine, feces from white-tailed deer for 66 months after exposure to low oral doses CWD-positive brain tissue or saliva. We analyzed seeding activity by using modified amyloid amplification assays incorporating iron oxide bead extraction,...
Bluetongue virus (BTV) is an economically important pathogen of ruminant species with worldwide prevalence. While many BTV infections are asymptomatic, animals symptomatic presentation deteriorate quickly the sickest succumbing to disease within one week. Animals that survive infection often require months recover. The immune response thought play a central role in controlling disease. Key understanding profiling vertebrate host immunological cellular and cytokine responses. Studies...
Bluetongue virus (BTV) is a prevalent midge-borne pathogen that infects ruminant species worldwide. BTV infections range from asymptomatic to lethal, with mechanisms determine the severity of infection remaining largely undefined. Although it relatively poorly understood, immune response thought be critical for both propagation disease as well resolution infection. To bridge this gap in knowledge, we infected cohorts sheep and muntjac deer two serotypes (BTV10 BTV17) longitudinal analysis...
An infectious agent’s pathogenic and transmission potential is heavily influenced by early events during the asymptomatic or subclinical phase of disease. During this phase, presence agent may be relatively low. important example Zika virus (ZIKV), which can cross placenta infect foetus, even in mothers with infections. These infections represent roughly 80 % all human Initial ZIKV pathogenesis studies were performed type I interferon receptor (IFNAR) knockout mice. Blunting response...
Abstract Hypoxia, the state of limited oxygen availability, is a frequent metabolic constraint in physiologic and pathologic states. The impact hypoxia hypoxic signaling on T cell differentiation remains incompletely defined, especially regarding inhibitory pathways that may be induced to limit hypoxia-associated tissue damage. Here we identified CD4 CD8 cells expressing IL-10 context inflammatory vivo. To test consequence availability generation cells, analyzed under conditions. Hypoxic...
Problem: Epidemiological studies in humans implicate maternal expression of different combinations KIRs, which are a family cell surface receptors that tune cellular activation status, as being predictive pregnancy complication onset (e.g., preeclampsia) and fetal health.This receptor is expressed both innate adaptive immune populations, including the most abundant types found pregnant uterus.Due to limitations studying human samples, mouse model was generated functionally homologous family,...
Recent studies have demonstrated dramatic shifts in metabolic supply-and demand ratios during inflammation. One major consequence of these changes is the induction localized tissue hypoxia inflammatory lesions (“inflammatory hypoxia”). As part adaptive immune response, T-cells are recruited to sites hypoxia, where they orchestrate responses. Due profound effects on gene regulation, we hypothesized that T cell differentiation transcriptionally controlled by hypoxia. To pursue this hypothesis,...
Abstract Human papillomavirus (HPV)-associated head and neck squamous cell carcinoma (HNSCC) is a fast-growing health problem in the United States. By analyzing transcriptomes of HPV-positive (HPV+) HPV-negative (HPV-) HNSCC patient tissues, we previously revealed that expression CXCL14, constitutively expressed basal epithelial cells, downregulated HNSCC. In contrast, proinflammatory chemokines, particularly CXCL1, CXCL2, CXCL8, are significantly upregulated both HPV+ HPV- HNSCCs. We...
Inflammatory bowel diseases, encompassing Crohn's disease and ulcerative colitis are characterized by persistent leukocyte tissue infiltration leading to perpetuation of an inappropriate inflammatory cascade. The neuronal guidance molecule, netrin-1 has recently been implicated in the orchestration trafficking during acute inflammation. We therefore hypothesized that could modulate activity a model disease. Netrin-1 was induced intestinal inflammation mice exposed experimental colitis....