A5092934243- interferon and immune responses
- Influenza Virus Research Studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Protein purification and stability
- Force Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
Rensselaer Polytechnic Institute
2023-2025
Summary Papain-like protease (PL pro ) from SARS-CoV-2 is a high-priority target for COVID-19 antiviral drug development. We present protocols PL production in Escherichia coli . expressed as fusion with the Saccharomyces cerevisiae Smt3 protein (SUMO), purified and obtained its native form upon hydrolysis, yields high 38 mg L -1 The protocol also provides isotope-enriched samples suitable NMR studies. Protocols are presented characterization by mass spectrometry, 1D 19 F-NMR 2D...
SUMMARY Influenza A and B viruses overcome the host antiviral response to cause a contagious often severe human respiratory disease. Here, integrative structural biology biochemistry studies on non- protein 1 of influenza virus (NS1B) reveal previously unrecognized viral mechanism for innate immune evasion. Conserved basic groups its C-terminal domain (NS1B-CTD) bind 5’- triphosphorylated double-stranded RNA (5’ppp-dsRNA), primary pathogen-associated feature that activates retinoic...
Abstract Influenza A and B viruses overcome the host antiviral response to cause a contagious often severe human respiratory disease. Here, integrative structural biology biochemistry studies on non-structural protein 1 of influenza virus (NS1B) reveal previously unrecognized viral mechanism for innate immune evasion. Conserved basic groups its C-terminal domain (NS1B-CTD) bind 5’-triphosphorylated double-stranded RNA (5’ppp-dsRNA), primary pathogen-associated feature that activates retinoic...