A5009603517- Ion Transport and Channel Regulation
- Connexins and lens biology
- Barrier Structure and Function Studies
- Neuroscience of respiration and sleep
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebrospinal fluid and hydrocephalus
- Ion channel regulation and function
- Cellular transport and secretion
- Retinal Development and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Neonatal Respiratory Health Research
- Lysosomal Storage Disorders Research
- Fetal and Pediatric Neurological Disorders
- Connective tissue disorders research
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Trypanosoma species research and implications
- Parkinson's Disease Mechanisms and Treatments
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Neurogenetic and Muscular Disorders Research
University of Oslo
2016-2024
657 Oslo
2020
Oslo University Hospital
2016
Abstract Waste from the brain has been shown to be cleared via perivascular spaces through so-called glymphatic system. According this model cerebrospinal fluid (CSF) enters in of arteries, crosses astrocyte endfoot layer, flows parenchyma collecting waste that is subsequently drained along veins. Glymphatic clearance dependent on astrocytic aquaporin-4 (AQP4) water channels are highly enriched endfeet. Even though polarized expression AQP4 endfeet thought crucial importance for CSF influx,...
Aquaporin-4 (AQP4) is the main water channel in brain and enriched perivascular astrocyte processes abutting microvessels. There a rich literature on role of AQP4 experimental stroke. While its oedema formation following middle cerebral artery occlusion (MCAO) has been studied extensively, specific impact infarct volume remains unclear. This study investigated effects total partial deletion mice subjected to distal medial (dMCAO) occlusion. Compared MCAO, this model induces smaller infarcts...
Biallelic PIGT variants were previously reported in seven patients from three families with Multiple Congenital Anomalies-Hypotonia Seizures Syndrome 3 (MCAHS3), characterized by epileptic encephalopathy, hypotonia, global developmental delay/intellectual disability, cerebral and cerebellar atrophy, craniofacial dysmorphisms, skeletal, ophthalmological, cardiac, genitourinary abnormalities. We report a novel homozygous missense variant c.1079G>T (p.Gly360Val) two brothers several of the...
The water channel protein aquaporin-4 (AQP4) and the gap junction forming proteins connexin-43 (Cx43) connexin-30 (Cx30) are astrocytic critically involved in brain ion homeostasis. While AQP4 is mainly flux across endfeet membranes, junctions provide syncytial coupling allowing intercellular exchange of water, ions, other molecules. We have previously shown that mice with targeted deletion Aqp4 display enhanced junctional between astrocytes. Here, we investigate whether uncoupling syncytium...
There is an urgent need to better understand the mechanisms involved in scar formation brain. It well known that astrocytes are critically engaged this process. Here, we analyze incipient one week after a discrete ischemic insult cerebral cortex. We show infarct border zone characterized by pronounced changes organization and subcellular localization of major astrocytic protein AQP4. Specifically, there loss AQP4 from endfoot membranes anchor pericapillary basal laminae disassembly...
More than 90% of the cases Parkinson's disease have unknown etiology. Gradual loss dopaminergic neurons substantia nigra is main cause morbidity in this disease. External factors such as environmental toxins are believed to play a role cell loss, although selective vulnerability remains unknown. We previously shown that aquaglyceroporin AQP9 expressed and astrocytes rodent brain. permeable broad spectrum substrates including purines, pyrimidines, lactate, addition water glycerol. Here we...
Aquaporin-4 (AQP4) is critically involved in brain water and volume homeostasis has been implicated a wide range of pathological conditions. Notably, evidence accrued to suggest that AQP4 plays proinflammatory role by promoting release astrocytic cytokines activate microglia other astrocytes. Neuroinflammation hallmark Parkinson's disease (PD), we have previously shown astrocytes substantia nigra (SN) are enriched relative cortical astrocytes, their complement further increased following...
Abstract Retinal Müller cells are highly polarized macroglial with accumulation of the aquaporin-4 (AQP4) water channel and inwardly rectifying potassium K ir 4.1 at specialized endfoot membrane domains abutting microvessels corpus vitreum. Proper homeostasis in retina depends on these specializations. Here we show that targeted deletion β1-syntrophin leads to a partial loss AQP4 from perivascular cell endfeet concomitant both α1- causes near complete subvitreal membranes. α1-syntrophin is...
Aquaporin-4 (AQP4) is the predominant water channel in brain; it enriched astrocytic foot processes abutting vessels where anchored through an interaction with dystrophin-associated protein (DAP) complex. Enhanced expression concomitant mislocalization of AQP4 along astrocyte plasma membranes a hallmark several neurological conditions. Thus, there urgent need to identify which signaling pathways dictate microdistribution. Here we show that canonical bone morphogenetic proteins (BMPs),...
More than 20 years have passed since the first demonstration of Aquaporin-9 (AQP9) in brain. Yet its precise localization and function brain tissue remain unresolved. In peripheral tissues, AQP9 is expressed leukocytes where it involved systemic inflammation processes. this study, we hypothesized that plays a proinflammatory role brain, analogous to periphery. We also explored whether Aqp9 microglial cells, which would be supportive hypothesis. Our results show targeted deletion...
Abstract Proper function of the retina depends heavily on a specialized form retinal glia called Müller cells. These cells carry out important homeostatic functions that are contingent their polarized nature. Specifically, cell endfeet contact microvessels and corpus vitreum show tenfold higher concentration inwardly rectifying potassium channel K ir 4.1 than other plasma membrane domains. This highly selective enrichment allows K+ to be siphoned through endfoot membranes in special spatial...
There is an urgent need to better understand the mechanisms involved in scar formation brain. It well known that astrocytes are critically engaged this process. Here we analyze in-cipient one week after a discrete ischemic insult cerebral cortex. We show infarct border zone characterized by pronounced changes organization and subcellular localization of major astrocytic protein AQP4. Specifically there loss AQP4 from endfoot membranes anchor pericapillary basal laminae disassembly...