A5008054353- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Catalysis for Biomass Conversion
- Enzyme Catalysis and Immobilization
- Lignin and Wood Chemistry
- Gene Regulatory Network Analysis
- Microplastics and Plastic Pollution
- CRISPR and Genetic Engineering
- Chemical Reactions and Isotopes
- Microbial Fuel Cells and Bioremediation
- biodegradable polymer synthesis and properties
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
Washington University in St. Louis
2019-2024
More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation PET waste has become a global pollution concern, motivating the urgent development technologies to valorize post-consumer PET. chemocatalytic and enzymatic approaches for depolymerizing its corresponding monomers opens up new opportunities upcycling through biological transformation. Here, we identify Rhodococcus jostii strain (RPET) that can directly use hydrolysate as...
Rhodococcus opacus is a bacterium that has high tolerance to aromatic compounds and can produce significant amounts of triacylglycerol (TAG). Here, we present iGR1773, the first genome-scale model (GSM) R. PD630 metabolism based on its genomic sequence associated data. The includes 1773 genes, 3025 reactions, 1956 metabolites, was developed in reproducible manner using CarveMe, evaluated through Metabolic Model tests (MEMOTE). We combine with two Constraint-Based Reconstruction Analysis...
Rhodococcus opacus is a nonmodel bacterium that well suited for valorizing lignin. Despite recent advances in our systems-level understanding of its versatile metabolism, studies gene functions at single level are still lagging. Elucidating organisms challenging due to limited genetic engineering tools convenient use. To address this issue, we developed simple repression system based on CRISPR interference (CRISPRi). This uses T7 RNA polymerase express small guide RNA, demonstrating improved...
Abstract Rhodococcus opacus PD630 has considerable potential as a platform for valorizing lignin due to its innate “biological funneling” pathways. However, the transcriptional regulation of aromatic catabolic pathways and mechanisms controlling operons in response different mixtures are still underexplored. Here, we identified studied transcription factors degradation using GFP-based sensors comprehensive deletion analyses. Our results demonstrate that funneling phenol, guaiacol,...
Abstract Lignocellulosic biomass is currently underutilized, but it offers promise as a resource for the generation of commercial end-products, such biofuels, detergents, and other oleochemicals. Rhodococcus opacus PD630 an oleaginous, Gram-positive bacterium with exceptional ability to utilize recalcitrant aromatic lignin breakdown products produce lipid molecules triacylglycerols (TAGs), which are important biofuel precursor. Lipid carbon storage accumulate only under growth-limiting low...
Chemical depolymerization of lignin is a non‐selective process that often generates wide distribution product compounds, denoted herein as breakdown products (LBPs). To address this limitation, we developed hybrid conversion approach employs lignin‐first catalytic on biomass and subsequent microbial upgrading. A Pd/C catalyst was used for reductive fractionation (RCF) poplar biomass, Rhodococcus opacus PD630 (R. PD630) then cultivated the resulting LBPs. This RCF increases total utilization...