A5061080905- Marine Biology and Environmental Chemistry
- Marine Ecology and Invasive Species
- Cephalopods and Marine Biology
- Planarian Biology and Electrostimulation
- Insect and Arachnid Ecology and Behavior
- Silk-based biomaterials and applications
- Adhesion, Friction, and Surface Interactions
- Developmental Biology and Gene Regulation
- Aquaculture disease management and microbiota
- Genetics, Aging, and Longevity in Model Organisms
- Environmental Toxicology and Ecotoxicology
- Animal Behavior and Reproduction
- Marine Bivalve and Aquaculture Studies
- Aquatic Invertebrate Ecology and Behavior
- Chromosomal and Genetic Variations
- Aquatic Ecosystems and Phytoplankton Dynamics
- Advanced biosensing and bioanalysis techniques
- Marine animal studies overview
- Marine Invertebrate Physiology and Ecology
- Studies on Chitinases and Chitosanases
- Hemiptera Insect Studies
- Aquaculture Nutrition and Growth
- Polymer Surface Interaction Studies
- Water Treatment and Disinfection
- Molecular Junctions and Nanostructures
Evides (Netherlands)
2023-2024
Universität Innsbruck
2014-2021
Humboldt-Universität zu Berlin
2009
Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, lack transgenesis methods considerably hampers their wider use. Here we report development a method for Macrostomum lignano, basal flatworm with excellent regeneration capacity. We demonstrate that microinjection DNA constructs into fertilized one-cell stage eggs, followed by low dose irradiation, frequently results in random...
Free-living flatworms, in both marine and freshwater environments, are able to adhere release from a substrate several times within second. This reversible adhesion relies on adhesive organs comprised of three cell types: an gland cell, releasing anchor which is modified epidermal responsible for structural support. However, nothing currently known about the molecules that involved this process. In study we present detailed morphology free-living flatworm Macrostomum lignano. About 130...
The flatworm Macrostomum lignano features a duo-gland adhesive system that allows it to repeatedly attach and release from substrates in seawater within minute. However, little is known about the molecules involved this temporary adhesion. In study, we show attachment of M. relies on secretion two large proteins, adhesion protein 1 (Mlig-ap1) Mlig-ap2. We revealed both proteins are expressed gland cells their distribution footprints was spatially restricted. RNA interference knockdown...
Phenotypic plasticity can enable organisms to produce optimal phenotypes in multiple environments. A crucial life history trait that is often highly plastic sex allocation, which simultaneous hermaphrodites describes the relative investment into male versus female functions. Theory predicts-and morphological evidence supports-that greater function favoured with increasing group size, due importance of sperm competition for reproductive success. Here, we performed a genome-wide gene...
Temporal and spatial characterization of gene expression is a prerequisite for the understanding cell-, tissue-, organ-differentiation. In multifaceted approach to investigate in tail plate free-living marine flatworm Macrostomum lignano, we performed posterior-region-specific situ hybridization screen, RNA sequencing (RNA-seq) regenerating animals, functional analyses selected tail-specific genes. The screen revealed transcripts expressed antrum, cement glands, adhesive organs, prostate...
Tunicates populate a great variety of marine underwater substrates worldwide and represent significant concern in shipping aquaculture. Adhesives are secreted from the anterior papillae their swimming larvae, which attach metamorphose into permanently adhering, filter-feeding adults. We recently described cellular composition sensory adhesive organ model tunicate Ciona intestinalis detail. Notably, secretions collocytes accumulate at tip contain glycoproteins. Here, we further explore...
PIWI proteins and piRNA pathways are essential for transposon silencing some aspects of gene regulation during animal germline development. In contrast to most species, flatworms also express PIWIs piRNAs in somatic stem cells, where they required tissue renewal regeneration. Here, we have identified characterized the emerging model flatworm Macrostomum lignano . We found that M. encodes at least three proteins. One these, Macpiwi1, acts as a key component canonical pathway cells. Knockdown...
Along with sperm, in many taxa ejaculates also contain large numbers of seminal fluid proteins (SFPs). SFPs and sperm are transferred to the mating partner, where they thought play key roles mediating post-mating sexual selection. They modulate partner's behavior physiology ways that influence reproductive success both partners, thus potentially leading conflict. Despite presumed general functional evolutionary significance SFPs, their identification characterization has date focused on just...
Flatworms can very rapidly attach to and detach from many substrates. In the presented work, we analysed adhesive system of marine proseriate flatworm Minona ileanae. We used light-, scanning- transmission electron microscopy analyse morphology organs, which are located at ventral side tail-plate. performed transcriptome sequencing differential RNA-seq for identification tail-specific transcripts. Using in situ hybridization expression screening, identified nine transcripts that were...
Abstract Members of the Aeromonas genus are commonly found in natural aquatic ecosystems. However, they also frequently present non-chlorinated drinking water distribution systems. High densities these bacteria indicate favorable conditions for microbial regrowth, which is considered undesirable. Studies have indicated that presence associated with loose deposits and invertebrates, specifically Asellus aquaticus . Therefore, a potential source nutrients nutrient poor environments chitin,...
Invertebrates, including Asellidae, are part of the natural ecosystem drinking water distribution system (DWDS) and known to cause a nuisance consumers. In addition, recently, potential role species Asellus aquaticus (L. 1758) in regrowth Aeromonas bacteria was published. is included Dutch guidelines as process parameter, guideline values regularly exceeded. Although neither A. nor associated with health risks, Evides utility shows strong interest possible reasons for these exceedances...
Many free-living flatworms have evolved a temporary adhesion system, which allows them to quickly attach and release from diverse substrates. In the marine Macrostomum lignano, morphology of adhesive system adhesion-related proteins been characterised. However, little is known about how performed in other aquatic environments. Here, we 3D reconstruction M. lignano organ compared it five selected Macrostomum, representing two marine, one brackish, freshwater species. We protein domains...
ABSTRACT Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration and tissue patterning. However, lack transgenesis methods significantly hampers their wider use. Here we report development a method for Macrostomum lignano , basal flatworm with excellent capacity. We demonstrate that microinjection DNA constructs into fertilized one-cell stage eggs, followed by low dose irradiation, frequently results in random integration...
Secretome proteomics reveals metabolic networks of Aeromonas strains for utilizing chitin as the sole nutrient source.