A5065403604- Cleft Lip and Palate Research
- Click Chemistry and Applications
- Craniofacial Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Facial Nerve Paralysis Treatment and Research
- Head and Neck Surgical Oncology
- Advanced biosensing and bioanalysis techniques
- Neurofibromatosis and Schwannoma Cases
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Mycobacterium research and diagnosis
- Genomic variations and chromosomal abnormalities
- Organophosphorus compounds synthesis
- HER2/EGFR in Cancer Research
- Tuberculosis Research and Epidemiology
- RNA modifications and cancer
- Enzyme function and inhibition
- Nasal Surgery and Airway Studies
- Genomics and Phylogenetic Studies
- Tracheal and airway disorders
National Dong Hwa University
2021-2024
Chang Gung Memorial Hospital
1989-2021
Taipei Medical University Hospital
2018-2021
Erasmus MC
2018-2020
Rotterdam University of Applied Sciences
2020
University Medical Center
2020
University Hospital and Clinics
2020
Vancouver Biotech (Canada)
2018
TRIUMF
2018
Genomics Research Center, Academia Sinica
2010-2016
We herein describe a flexible synthesis of small library 68Ga-labeled CAIX-targeted molecules via an orthogonal 2-cyanobenzothiazole (CBT)/1,2-aminothiol click reaction. Three novel CBT-functionalized chelators (1⁻3) were successfully synthesized and labeled with the positron emitter gallium-68. Cross-ligation between pre-labeled bifunctional (BFCs) 1,2-aminothiol-acetazolamide derivatives (8 9) yielded six new CAIX ligands high radiochemical yields. The reaction conditions optimized to...
A new synthetic approach toward the bacterial transglycosylase substrates, Lipid II (1) and IV (2), is described. The key disaccharide was synthesized using concept of relative reactivity value (RRV) elaborated to by conjugation with appropriate oligopeptides pyrophosphate lipids. Interestingly, results from our HPLC-based functional TGase assay suggested has a higher affinity for enzyme than II.
The development of iminocyclitol-based small molecule libraries against a bacterial TGase is described. An iminocyclitol was conjugated with pyrophosphate mimic using either 1,3-dipolar cycloaddition or reductive amination reaction, which then condensed variety lipophilic carboxylic acids in an amide bond coupling to generate desired molecular library. With assistance microtiter plate-based combinatorial chemistry and situ screening, potential inhibitor, the first potent inhibitor TGases...
Abstract A flexible synthetic strategy toward the preparation of diverse N ‐substituted muramyl dipeptides ( MDPs) from different protected monosaccharides is described. The MDPs include ‐acetyl MDP and ‐glycolyl MDP, known NOD2 ligands, this methodology allows for structural variation at six positions, including muramic acid, peptide, moieties. capacity these molecules to activate human in innate immune response was also investigated. It found that addition methyl group C 1 position...
A feasible synthetic approach toward the Mycobacterium tuberculosis (Mtb) N-glycolyl lipid II-like molecule 1 is described. Compound bears pendant undecaprenol and l-lysin moieties instead of naturally occurring decaprenol meso-diaminopimelic acid, which are not readily available. Functionalization with a fluorophore on peptide side chain gave 14, was found to be recognized as an Mtb TGase substrate. This result suggests it has tremendous utility for mechanistic studies, characterization...
Breaking down barriers: A rapid, inexpensive preparation of the structurally complex mycobacterial N-glycolyl Lipid I, II, and their analogues from a range different synthetic N-glycinyl Park's nucleotides is described (see scheme). The biotransformations were catalyzed by readily available biocatalyst obtained bacterial cell-free membrane fraction. unnatural II was found to be substrate Mycobacterium tuberculosis (Mtb) transglycosylase, PonA, I weak inhibitor against PonA. As service our...
An open tip rhinoplasty was used to correct moderate severe secondary cleft lip nasal deformities in 122 patients from January 1986 1988. The results of the surgery on 92 who were followed for 1 year or more evaluated. When performed after age 3 years, 60% had a satisfactory result. pathology deformity included 32% with deficiencies lining, 35% needed framework support such as columellar strut, and 45% required correction deficiencies. Patients using postoperative stent 71% result compared...
Van der Woude syndrome, characterized by lip pits and cleft lip/ palate, presents with variable expressions. This retrospective study was designed to obtain a better understanding of its clinical pattern in Taiwanese patients.Of 13,147 patients treated at the Chang Gung Craniofacial Center from 1976 2004, there were 64 syndrome. Clinical expressions family histories collected analyzed.The male female ratio 1:1. The majority had complete types. Severe found among patients, 52% having...
Nuclear and optical dual-modality probes can be of great assistance in prostate cancer localization, providing the means for both preoperative nuclear imaging intraoperative surgical guidance. We developed a series based on backbone established GRPR-targeting radiotracer NeoB. The inverse electron demand Diels–Alder reaction was used to integrate sulfo-cyanine 5 dye. Indium-111 radiolabeling, stability studies competition binding assay were carried out. Pilot biodistribution performed PC-3...
FASTR3D is a web-based search tool that allows the user to fast and accurately PDB database for structurally similar RNAs. Currently, it input three types of queries: (i) code an RNA tertiary structure (default), optionally with specified residue range, (ii) secondary structure, primary sequence, in dot-bracket notation (iii) sequence FASTA format. In addition, can run specifying additional filtering options: released date structures database, experimental methods used determine their least...
Abstract Preparation of Lipid II analogues containing an enzymatically uncleavable 1‐ C ‐glycoside linkage between the disaccharide moiety and pyrophosphate‐ or pyrophosphonate‐lipid is described. The synthesis a common ‐vinyl intermediate has been developed that allows easy preparation both elongated sugar‐phosphate bond sugar‐phosphonate moiety, which are coupled with polyprenyl phosphate to give desired molecules. Inhibition studies show how subtle structural modification results in...
Abstract Systematic structural modifications of the muramic acid, peptide and nucleotide moieties Park’s were performed to investigate substrate specificity B. subtilis MraY (MraY BS ). It was found that simplest analogue only bearing first two amino acids, l -alanine- iso - d -glutamic could function as a substrate. Also, acid group attached C α play an important role for activity. Epimerization 4-hydroxyl modification at 5-position uracil in both dramatically impair their Unexpectedly, on...
Abstract We herein reported a concise synthesis of new class tetrazolyl‐benzothiazoles (TBTs). This was achieved via microwave‐assisted ANRORC method followed by an acid‐catalyzed [2 + 3] cycloaddition. The TBT core elaborated with diverse substituents at the N ‐1 or ‐2 position on tetrazole ring to create compound library for further bioactivity evaluation. newly synthesized TBT‐based compounds were assessed their inhibitory effects against human breast cancer cell lines, MCF‐7 and...
R3D-BLAST is a BLAST-like search tool that allows the user to quickly and accurately against PDB for RNA structures sharing similar substructures with specified query structure. The basic idea behind all 3D deposited in are first encoded as 1D structural sequences using alphabet of 23 distinct nucleotide conformations, BLAST then applied these those whose substructure. takes input an structure format outputs hits graphical display show their superposition. In addition, each substructure hit...
Abstract Herein, we describe a synthetic strategy for the regioselective labeling of peptides by using bioorthogonal click reaction between 2‐cyanobenzothiazole (CBT) and 1,2‐aminothiol moiety. This methodology allows facile site‐specific modification with various imaging agents, including fluorophores radioisotope‐containing prosthetic groups. We investigated feasibility an early‐stage incorporation dipeptide 1 into targeting vectors, such as c[RGDyK(C)] HER2 pep , during solid‐phase...
Flipping of this hydroxyl group dramatically changes the molecular character from a TG substrate to inhibitor!
A case of left facial elephantiasis neurofibromatosa was treated with near-total excision and skin graft. Results were favorable. Repositioning canthal ligaments, preserving part relatively normal masseter muscle primary nasal reconstruction forehead flap performed. The graft over the myxomatous successful. Troublesome bleeding avoided or controlled by incising skin, subperiosteal dissection, hypotensive anesthesia, chromic catgut suture ligature.