A5105839051- Immune Response and Inflammation
- Nitric Oxide and Endothelin Effects
- NF-κB Signaling Pathways
- Adenosine and Purinergic Signaling
- Protein Kinase Regulation and GTPase Signaling
- Cell death mechanisms and regulation
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Ion channel regulation and function
- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- Neuropeptides and Animal Physiology
- PARP inhibition in cancer therapy
- Venomous Animal Envenomation and Studies
- Mast cells and histamine
National Taiwan University
2016-2025
Taipei Medical University
2014-2025
Sun Yat-sen University
2021-2025
Third Affiliated Hospital of Sun Yat-sen University
2021-2025
Longgang Central Hospital
2025
Shantou University
2013-2024
Shantou University Medical College
2013-2024
National Defense Medical Center
2020-2023
Academia Sinica
2020-2023
Cancer Hospital of Shantou University Medical College
2022-2023
The antidiabetic drug metformin exerts chemopreventive and antineoplastic effects in many types of malignancies. However, the mechanisms responsible for actions appear diverse may differ different cancer. Understanding molecular cellular specific cancers is important to optimize strategy treatment cancer types. Here, we investigate vitro vivo on esophageal squamous cell carcinoma (ESCC) cells. Metformin selectively inhibited growth ESCC tumor cells but not immortalized noncancerous...
Abstract Esophageal cancer (EC) is a type of aggressive without clinically relevant molecular subtypes, hindering the development effective strategies for treatment. To define subtypes EC, we perform mass spectrometry-based proteomic and phosphoproteomics profiling EC tumors adjacent non-tumor tissues, revealing catalog proteins phosphosites that are dysregulated in ECs. The cohort stratified into two subtypes—S1 S2—based on analysis, with S2 subtype characterized by upregulation...
Esophageal cancer is the seventh most common in world. Although traditional treatment methods such as radiotherapy and chemotherapy have good effects, their side effects drug resistance remain problematic. The repositioning of function provides new ideas for research development anticancer drugs. We previously showed that Food Drug Administration-approved sulconazole can effectively inhibit growth esophageal cells, but its molecular mechanism not clear. Here, our study demonstrated had a...
Both the nitrite and prostaglandin E 2 (PGE ) release caused by lipopolysaccharide (LPS) in J774 macrophages are inhibited SB 203580, a specific p38 mitogen‐activated protein kinase (MAPK) inhibitor, concentration‐dependent manner. The 50% inhibitory concentration (IC 50 for PGE responses was 1 μm 0·5 μm, respectively. Inhibition marked following simultaneous treatment with 203580 LPS, much reduced when added 6 hr after LPS treatment. In parallel, induction of inducible NO synthase (iNOS)...
Abstract Δ 9 ‐Tetrahydrocannabinol (Δ ‐THC) is the major psychoactive component of marijuana and elicits pharmacological actions via cannabinoid receptors. Anandamide (AEA) 2‐arachidonoyl‐glycerol (2‐AG) are endogenous ligands for receptors, which because their structural similarities to arachidonic acid (AA), AEA, 2‐AG could serve as substrates lipoxygenases cyclooxygenases (COXs) that metabolize polyunsaturated fatty acids potent bioactive molecules. In this study, we have compared effects...
Abstract An inflammatory response in the central nervous system mediated by activation of microglia is a key event early stages development neurodegenerative diseases. Silymarin polyphenolic flavanoid derived from milk thistle that has anti‐inflammatory, cytoprotective and anticarcinogenic effects. In this study, we first investigated neuroprotective effect silymarin against lipopolysaccharide (LPS)‐induced neurotoxicity mesencephalic mixed neuron–glia cultures. The results showed...
Objectives— Diabetes mellitus causes endothelial dysfunction. The precise molecular mechanisms by which hyperglycemia apoptosis in cells are not yet well understood. aim of this study was to explore the role cyclooxygenase-2 (COX-2) and possible involvement phosphoinositide 3-kinase (PI3K) signaling high glucose (HG)–induced human umbilical vein (HUVECs). Methods Results— For detection apoptosis, morphological Hoechst staining Annexin V/propidium iodide were used. Glucose upregulated COX-2...
Abstract NLRP3 is the most crucial member of NLR family, as it detects existence pathogen invasion and self-derived molecules associated with cellular damage. Several studies have reported that excessive inflammasome-mediated caspase-1 activation a key factor in development diseases. Recent Syk involved pathogen-induced inflammasome activation; however, detailed mechanism linking to remains unclear. In this study, we showed mediates stimuli-induced processing procaspase-1 consequent...
Diabetic retinopathy (DR) and age-related macular degeneration (AMD) are two important leading causes of acquired blindness in developed countries. As accumulation advanced glycation end products (AGEs) retinal pigment epithelial (RPE) cells plays an role both DR AMD, the methylglyoxal (MGO) within AGEs exerts irreversible effects on protein structure function, it is crucial to understand underlying mechanism MGO-induced RPE cell death. Using ARPE-19 as model, this study revealed that MGO...
Abstract Purpose: Transcriptionally induced chimeric RNAs are an important emerging area of research into molecular signatures for biomarker and therapeutic target development. Salivary exosomes represent a relatively unexplored, but convenient, noninvasive cancer discovery. However, the potential cancer-derived exosomal in saliva as biomarkers is unknown. Here, we explore clinical utility salivary GOLM1-NAA35 RNA (seG-NchiRNA) esophageal squamous cell carcinoma (ESCC). Experimental Design:...
Oxidative stress is a major factor in retinal pigment epithelium (RPE) cells injury that contributes to age-related macular degeneration (AMD). NaIO3 an oxidative toxic agent and its selective RPE cell damage makes it as reproducible model of AMD. Although inducer, the roles ROS NaIO3-elicited signaling pathways viability have not been elucidated, effect on autophagy remains elusive. In human ARPE-19 cells, we used Annexin V/PI staining determine viability, immunoblotting protein expression...
Diabetic retinopathy (DR) is a major cause of blindness in adult, and the accumulation advanced glycation end products (AGEs) pathologic event DR. Methylglyoxal (MGO), highly reactive dicarbonyl compound, precursor AGEs. Although therapeutic potential metformin for disorders has recently been elucidated, possibly through AMPK activation, it remains unknown how directly affects MGO-induced stress response retinal pigment epithelial cells. Therefore, this study, we compared effects activator...
We have developed a multistep route to the fabrication of virus assembled nanostructures with chemoselective protein-to-surface linkers synthesized by an efficient solid-phase method. These were used create patterns 30-to-50-nm-width-lines scanning probe nanolithography. Genetically modified cow pea mosaic unique cysteine residues at specific locations on their capsomers through covalent linkage these patterns. The morphology structures line characterized atomic force microscopy was found be...
1. Although capsaicin analogs might be a potential strategy to manipulate inflammation, the mechanism is still unclear. In this study, effects and action mechanisms of vanilloid on iNOS COX-2 expression were investigated in RAW264.7 macrophages. 2. Capsaicin resiniferatoxin (RTX) can inhibit LPS- IFN-gamma-mediated NO production, protein mRNA with similar IC50 values around 10 microm. 3. also transcriptionally inhibited PMA-induced PGE2 production. However, effect exhibited higher potency...
Ahead of Print article withdrawn by publisher. A previous report showed that the pan-caspase inhibitor zVAD can induce necrosis accompanied autophagosome formation in L929 fibrosarcoma cells. Such autophagic cell death relies on caspase 8 inhibition and ROS accumulation. Since connection these molecules is still poorly understood, we explored underlying signaling cascades this event. First, confirmed stimulate LC3 cleavage, beclin 1 gene expression, formation, accumulation Antioxidants,...