A5101560764- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Prostate Cancer Treatment and Research
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Forensic Entomology and Diptera Studies
- Wnt/β-catenin signaling in development and cancer
- Forensic and Genetic Research
- Inflammasome and immune disorders
- Circadian rhythm and melatonin
- Fibroblast Growth Factor Research
- Dendrimers and Hyperbranched Polymers
- Congenital limb and hand anomalies
- Genetic diversity and population structure
- Immune Response and Inflammation
- Characterization and Applications of Magnetic Nanoparticles
- Physiological and biochemical adaptations
- Chemokine receptors and signaling
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
Shanghai Medical College of Fudan University
2014-2024
Hunan Normal University
2023
Beth Israel Deaconess Medical Center
2023
Harvard University
2017-2023
Dana-Farber Cancer Institute
2023
Broad Institute
2023
Wuhan University of Technology
2018-2022
Inner Mongolia University
2022
Sun Yat-sen University Cancer Center
2021
Sun Yat-sen University
2021
Background Neutrophils play a controversial role in tumor development. The function of programmed cell death-1 ligand (PD-L1 + ) neutrophils, however, may inhibit the cytotoxicity anti-tumor immunity. In this study, we elucidate stimulators PD-L1 neutrophils microenvironment (TME) and explore optimal combination to enhance effect lenvatinib by inhibiting hepatocellular carcinoma. Methods Neutrophil infiltration after treatment was examined with RNA sequencing multicolor flow cytometry...
Malignancies arising from mutation of tumor suppressors have unexplained tissue proclivity. For example, BAP1 encodes a widely expressed deubiquitinase for histone H2A, but germline mutations are predominantly associated with uveal melanomas and mesotheliomas. We show that inactivation causes apoptosis in mouse embryonic stem cells, fibroblasts, liver, pancreatic not melanocytes mesothelial cells. Ubiquitin ligase RNF2, which silences genes by monoubiquitinating promoted BAP1-deficient cells...
Abstract Determining the postmortem interval ( PMI ) is important in criminal, civil, and forensic cases. We examined feasibility of using transcript abundances m RNA s, 18 S r , U 6 sn micro s as a means to estimate . removed spleen tissues from rats at different under 4°C or 25°C gene these samples by RT ‐q PCR Using algorithm geNorm, we found that be appropriate control markers because they were less affected temperature. also characterized relationships between observed levels...
Synthesis schematics of DSA and 6-TG-DSA as well processes PPN self-assembly its pH/GSH dual stimuli-response release the conjugated 6-TG.
We developed a universal method termed OnCELISA to detect cytokine secretion from individual cells by applying capture technology on the cell membrane. uses fluorescent magnetic nanoparticles as assay reporters that enable detection single-cell level in microscopy and flow cytometry fluorimetry ensembles. This system is flexible can be modified different cytokines broad range of cytokine-secreting cells. Using we have been able select sort highly identify expression profiles populations...
Emerging data indicate that antipsychotic treatment causes brain volume loss and astrocyte death, but the mechanisms remain elusive. Pyroptosis, inflammatory cell death characterized by formation of bodies, increased expression nod-like receptor proteins (NLRPs) such as NLRP3, activation caspases gasdermin D (GSDMD) are largely associated with innate immunity, inflammation, injury/death. However, main effect antipsychotics on pyroptotic signaling molecular obscure. In present study, 72-h...
Aberrant alternative splicing (AS) is increasingly linked to cancer; however, how AS contributes cancer development still remains largely unknown. events (ASEs) are regulated by RNA-binding proteins (RBPs) whose ability can be modulated a variety of genetic and epigenetic mechanisms. In this study, we used computational framework investigate the roles transcription factors (TFs) on regulating RBP-AS interactions. A total 6519 TF–RBP–AS triplets were identified, including 290 TFs, 175 RBPs,...
Obesity has become a major public health issue which is caused by combination of genetic and environmental factors. Genome-wide DNA methylation studies have identified that at Cytosine-phosphate-Guanine (CpG) sites are associated with obesity. However, subsequent functional validation the results from these been challenging given high number reported associations. In this study, we applied an integrative analysis approach, aiming to prioritize drug development candidate genes many CpGs....
<div>Abstract<p>Wnt signaling driven by genomic alterations in genes including <i>APC</i> and <i>CTNNB</i>, which encodes β-catenin, have been implicated prostate cancer development progression to metastatic castration-resistant (mCRPC). However, nongenomic drivers downstream effectors of Wnt the therapeutic potential targeting this pathway not fully established. Here we analyzed Wnt/β-catenin identified distinct from those found other tissues, aryl...
Human induced pluripotent stem cells (iPSCs) have good multi-lineage differentiation potential, which is an ideal model for studying the pathogenesis of diseases and drug screening.Here, we generated iPSC line, SHUPLi002-A, from peripheral blood mononuclear (PBMCs) a healthy 28-year-old female individual using episomal vectors. SHUPLi002-A characterized by expression classical cell markers as well teratoma formation assays to evaluate their capacity in vivo.
Correction for ‘pH and reduction-activated polymeric prodrug nanoparticles based on a 6-thioguanine-dialdehyde sodium alginate conjugate enhanced intracellular drug release in leukemia’ by Yanming Wan <italic>et al.</italic>, <italic>Polym. Chem.</italic>, 2018, DOI: 10.1039/c8py00577j.
Mesenchymal stem cells (MSCs) were used widely as seed in tissue engineering blood vessel construction. However, the biological characteristics difference of different generation MSCs vitro culture is unknown, which laid a foundation for appropriate seeded selection In this report, isolated from SD rat bone marrow and identified by flow cytometry; cell growth curve test, surface antigen expression rate detection, cryopreservation resuscitation CD31 cycle analysis, adhesion on vascular...
<div>Abstract<p>Wnt signaling driven by genomic alterations in genes including <i>APC</i> and <i>CTNNB</i>, which encodes β-catenin, have been implicated prostate cancer development progression to metastatic castration-resistant (mCRPC). However, nongenomic drivers downstream effectors of Wnt the therapeutic potential targeting this pathway not fully established. Here we analyzed Wnt/β-catenin identified distinct from those found other tissues, aryl...