A5073049933- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Microbial infections and disease research
- Ovarian cancer diagnosis and treatment
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Vector-borne infectious diseases
- Streptococcal Infections and Treatments
- RNA modifications and cancer
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Enzyme Structure and Function
- Herpesvirus Infections and Treatments
- Evolutionary Algorithms and Applications
- Viral Infections and Immunology Research
- Reproductive System and Pregnancy
- Neural Networks and Applications
University College London
2011-2024
The Royal Free Hospital
2011-2024
Roland Hill (United Kingdom)
2024
Hubrecht Institute for Developmental Biology and Stem Cell Research
2021
Oncology Institute of Vojvodina
2012
Centro di Riferimento Oncologico
2012
Institute for Veterinary Medical Research
2010
Hungarian Academy of Sciences
2010
Ministry of Agriculture
2010
The University of Texas Medical Branch at Galveston
1996
The most common cause of epidemic pediatric respiratory disease, syncytial virus (RSV), stimulates interleukin-8 (IL-8) synthesis upon infecting airway epithelium, an event necessary for the development mucosal inflammation. We investigated mechanism enhanced IL-8 production in human A549 type II pulmonary epithelial cells. Infection with sucrose-purified RSV (pRSV) produced a time-dependent increase transcriptional initiation rate gene. Transient transfection promoter mutated binding site...
An emerging cellular immunotherapy for cancer is based on the cytolytic activity of natural killer (NK) cells against a wide range tumors. Although in vitro activation, or “priming,” NK by exposure to pro-inflammatory cytokines, such as interleukin (IL)-2, has been extensively studied, biological consequences cell activation response target interactions have not thoroughly characterized. We investigated co-incubation with K562, CTV-1, Daudi RPMI-8226, and MCF-7 tumor lines phenotype,...
Resting human NK cells require a two-stage activation process that we have previously described as "priming" and "triggering." NK-sensitive tumor provide both priming triggering signals. NK-resistant tumors evade lysis, mostly by failure to prime; however, recently reported cell line (CTV-1) primes resting but fails trigger lysis. In this article, report two additional leukemia lines prime are resistant Tumor-mediated is via CD2 binding ligand within CD15 on the cell. RAJI became susceptible...
Human and mouse natural killer (NK) cells have been shown to develop memory-like function after short-term exposure the cocktail of IL-12/15/18 or overnight co-culture with some tumor cell lines. The resulting retain enhanced lytic ability for up 7 days as well cryopreservation, NK (mlNK) induce complete remissions in patients hematological malignancies. No single phenotype has described mlNK physiological changes induced by cytokine tumor-priming which are responsible these functions not...
Turkeys and chickens were experimentally infected with a ureaplasma strain T-1001 isolated from turkey semen. Following infection, sero-fibrinous airsacculitis serological responses developed in turkeys of different ages.
Background: Tissue-specificity for fimbrial fallopian tube ovarian carcinogenesis remains largely unknown in BRCA1 mutation carriers. We aimed to assess the cell autonomous and cell-nonautonomous implications of a germline context cancer immunosurveillance CD3− CD56+ natural killer (NK) cells. Methods: Premenopausal carriers versus age-matched non-carriers were compared. Daily urinary 5β-pregnanediol levels used determine progesterone metabolomics across an cycle. Using peripherally acquired...
Summary Experimental infection of chickens and turkeys various ages was performed with a Ureaplasma chicken origin. The caused slight respiratory symptoms prominent airsacculitis peritonitis developed as well serological response the infected birds. Zusammenfassung Untersuchungen über die Pathogenität von Harnstoff-spaltenden Mykoplasmen im Geflügel Hühner und Truthühner verschiedener Altersstufen wurden mit einem Küken isolierten, Mykoplasmenstamm experimentell infiziert. Diese...
Pre‐tRNAs are transcribed as precursors which undergo maturation including cleavage of 5′ and 3′ extensions end CCA addition. The trailer is endonucleolytically removed by tRNase Z, a member the β‐lactamase superfamily metal‐dependent hydrolases. body enzyme consists four‐layer alpha‐beta sandwich conserved metal‐coordinating HxHxDH motif. tRNAse Z features unique protruding flexible arm (FA), an ααββ hand connected to ascending descending stalks, involved with recognition binding elbow...
We introduce poly-adenine CRISPR gRNA-based single-cell RNA-sequencing (pAC-Seq), a method that enables the direct observation of guide RNAs (gRNAs) in scRNA-seq. use pAC-Seq to assess phenotypic consequences CRISPR/Cas9 based alterations gene cis-regulatory regions. show is able detect cis-regulatory-induced alteration target expression even when biallelic loss occurs only ~5% cells. This low rate significantly increases number cells required changes regulatory genome, but can be...