A5101934315- Medicinal Plants and Bioactive Compounds
- Retinal Diseases and Treatments
- Advanced Glycation End Products research
- Biochemical effects in animals
- Flavonoids in Medical Research
- Neurological Disease Mechanisms and Treatments
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- Molecular Sensors and Ion Detection
- Pancreatic function and diabetes
- Psoriasis: Treatment and Pathogenesis
- Plant-based Medicinal Research
- Berberine and alkaloids research
- Neurological Disorders and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Endoplasmic Reticulum Stress and Disease
- Pain Mechanisms and Treatments
- Biomarkers in Disease Mechanisms
- Whipple's Disease and Interleukins
- Natural Antidiabetic Agents Studies
- Cancer-related molecular mechanisms research
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and disorders
Jinzhou Medical University
2015-2024
Kunming Medical University
2024
Guangxi Medical University
2019-2024
Air Force Medical University
2015
Hyperoxia-induced acute lung injury (HALI) is a critical life-threatening disorder characterized by severe infiltration immune cells and death of type II alveolar epithelial (AECII). However, little known about the relations between AECII in HALI. IL-17A pro-inflammatory cytokine mainly secreted Th17 cells, contributing to pathogenesis various inflammatory diseases. The present study investigated role cell-cell communication HALI, explored therapeutic effect salidroside (Sal, natural...
Naringin, an essential flavonoid, inhibits inflammatory response and oxidative stress in diabetes. However, whether naringin has beneficial effects on diabetic retinopathy (DR) remains unknown. Streptozotocin (STZ, 65 mg/kg) was intraperitoneally injected into male rats (8 weeks old weighting 200-250 g) to establish model, then (20, 40 or 80 mg/kg/day) the for twelve weeks. Glial fibrillary acidic protein (GFAP) level, thickness of ganglion cell layer (GCL) counts were assessed retina vivo....
Objective: This study aimed to establish a reliable and reproducible protocol for C6 glioma culture in vitro, which, bromodeoxyuridine (BrdU) labeling, immunohistochemical characterization of cells were used facilitate research improve the sensitivity specificity detecting proliferating tumor cells. Methods: resuscitated, cultured, labeled with BrdU at concentration 5 μg/mL. The viability was assessed using trypan blue staining. Cells subsequently fixed subjected staining Alexa Fluor...
To explore the effects and potential mechanisms of curcumin on retinal Müller cell in early diabetic rats.Diabetic rats were induced by a single intraperitoneal injection streptozotocin (STZ). Male Sprague-Dawley (SD) randomly assigned into 4 groups: control group (naïve SD administered with citric buffer), (STZ-diabetic rats), dimethyl sulfoxide (DMSO) (diabetic intraperitoneally mixture DMSO normal saline, once day) curcumin, 80mg/kg, day). Three months after diabetes onset,...
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers and causes death in developed countries. SphK2 overexpressed a number aggressive human carcinomas; however, expression profile potential function CRC are still unknown. In this study, we investigated tumoral tissue matched normal mucosae using quantitative real-time PCR (qRT-PCR), Western blot, immunohistochemistry. We also evaluated impact knockdown on cell proliferation metastasis vitro. was significantly upregulated as...
In recent years, molecular imprinting has emerged as a promising strategy for developing highly selective and sensitive sensors various analytes. this study, we present the fabrication characterization of an electrochemical sensor based on detection salidroside, bioactive compound found in Rhodiola rosea. The employs modified glassy carbon electrode functionalized with multi-walled nanotubes (MWCNTs) working electrode. molecularly imprinted polymer (MIP) was prepared using acrylamide...
Retinal ganglion cells (RGCs) are among the first neurons to undergo apoptosis in diabetic retinopathy (DR), with their relationship endoplasmic reticulum stress (ERS)-induced still unclear. While glucocorticoid-induced transcript 1 (GLCCI1) has been shown inhibit apoptosis, its role ERS-induced and mechanisms DR remain unclarified. Our findings indicated that GLCCI1 is predominantly localized cell layer downregulated DR. overexpression mitigated of RGCs swelling mitochondria under...
Chronic pain occurs in ~85-90% of chronic pancreatitis (CP) patients. However, as the pathogenesis CP remains to be fully understood, current therapies for remain inadequate. Emerging evidence has suggested that epigenetic modulations genes are involved pain. In present study, intrapancreatic trinitrobenzene sulfonic acid infusions were used establish a model rats. Mechanical allodynia was measured with von Frey filaments. Immunofluorescent staining analysis observe expression changes...
The Nogo receptor is an essential factor for neuronal apoptosis, but the changes in expression retina and effects of on retinal ganglion cell apoptosis diabetes mellitus remain unclear. We found that was mainly visible cells a rat model induced by streptozotocin. At 12 weeks after onset mellitus, Rho kinase significantly increased retina, apparent. When RNA interference used to suppress obviously inhibited, evidently reduced rats with mellitus. These results indicate upregulation important mechanism
To study how to effectively prevent or reduce renal injury caused by contrast agents in diabetic patients.Sprague Dawley (SD) rats were bred with a high-fat diet for eight weeks, then intraperitoneally injected Streptozotocin (STZ) prepare the diabetes model. Rats treated Iodixanol contrast-induced acute kidney (CIAKI) Moreover, 3-methyladenine (3-MA), an autophagy inhibitor, was administrated without Rapamycin treatment. Serum creatinine (SCr) and blood urea nitrogen (BUN) examined using...
Purpose: Diabetic retinopathy (DR) is a serious complication of diabetes mellitus, which nearly happens to all the diabetic sufferers. This study aims identify preliminary molecular regulation involved in therapeutic efficacy astragaloside IV (AS- IV) for DR. Methods: rat models were established and treated with AS-IV. Optical coherence tomography (OCT) Hematoxylin-eosin (HE) staining was employed demonstrate histopathological changes. The main targets AS-IV identified by searching from...
The present study aimed to investigate the expression of microRNA (miRNA)-23a in blood and tear samples from diabetic retinopathy (DR) patients. Blood were obtained 33 patients with proliferative DR. Additionally, a rat model DR was established. Reverse transcription-quantitative PCR used determine vascular endothelial growth factor (VEGF) mRNA miRNA-23a levels, while ELISA western blot analysis performed protein levels. Bioinformatics dual luciferase reporter assay predict validate...
Purpose This study aimed to investigate the effect of Raf-1 kinase inhibitory protein (RKIP) on diabetic retinal neurodegeneration in streptozotocin-treated rat model and high glucose-treated Müller cells.Methods Control rats were intravitreally injected with saline, RKIP gene overexpression lentivirus (oeRKIP) or negative control (RKIP-vector). Normal cells transfected lentivirus. Western blotting immunofluorescence assay utilized evaluate function expression RKIP, p38 mitogen-activated...