A5035663944- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Viral gastroenteritis research and epidemiology
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Animal Virus Infections Studies
- Allergic Rhinitis and Sensitization
- interferon and immune responses
- Immune responses and vaccinations
- Viral Infections and Immunology Research
- Eosinophilic Disorders and Syndromes
- Immune Cell Function and Interaction
- Polyamine Metabolism and Applications
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Eosinophilic Esophagitis
- Plant Virus Research Studies
- Electrostatic Discharge in Electronics
Western New England University
2018-2024
University of Michigan
2021-2024
Respiratory syncytial virus (RSV) infects most infants by two years of age. It can cause severe disease leading to an increased risk developing asthma later in life. Previously, our group has shown that RSV infection mice and promotes IL-1β production. Here, we characterized the role NLRP3-Inflammasome activation during adult neonates. We observed inhibition NLRP3 using small molecule inhibitor, MCC950, or genetically modified knockout (Nlrp3−/−) vivo led decreased lung immunopathology along...
Our studies have previously shown a role for persistent TSLP production in the lungs of mice after early-life respiratory syncytial virus (RSV) infection that leads to an altered immune phenotype, including accumulation "inflammatory" dendritic cells (DC). This study investigates driving systemic trained immunity DC RSV-infected mice. Bone marrow-derived DCs (BMDC) from at 4 wk postinfection showed enhanced expression costimulatory molecules and cytokines, Tslp, regulate cell function. The...
Abstract Mast cells (MCs) play critical roles in the establishment of allergic diseases. We recently demonstrated an unexpected, proinflammatory role for IL-10 regulating MC responses. enhanced activation and promoted IgE-dependent responses during food allergy. However, whether these effects extend to IgE-independent stimuli is not clear. In this article, we demonstrate that plays a driving IL-33–mediated stimulation expansion degranulation, ST2 expression, IL-13 production, phospho-relA...
Previous studies from our laboratory and others have established the dendritic cell (DC) as a key target of RSV that drives infection-induced pathology. Analysis RSV-induced transcriptomic changes in RSV-infected DC revealed metabolic gene signatures suggestive altered cellular metabolism. Reverse phase protein array (RPPA) data showed significantly increased PARP1 phosphorylation DC. Real-time analysis demonstrated glycolysis PARP1-/- after infection, confirming role for regulating Our show...
Abstract IgE-mediated mast cell (MC) activation is a critical component of allergic responses to oral Ags. Several T cell–derived cytokines have been shown promote MC reactivity, and we recently demonstrated role for the cytokine IL-10 in mediating during food allergy. In this study, further validate using Ab-mediated depletion. neutralization significantly attenuated responses, leading decreased accumulation activation, as well inhibition MC-mediated symptoms such diarrhea. This was...
This study investigates sex-associated systemic innate immune differences by examining bone marrow-derived dendritic cells (BMDCs). BMDC grown from 7-day-old mice show enhanced type-I interferon (IFN) signaling in female compared to male BMDC. Upon respiratory syncytial virus (RSV) infection of mice, a significantly altered phenotype at 4 weeks post-infection is observed sex-dependent manner. The alterations include heightened Ifnb/ interleukin (Il12a) and IFNAR1+ expression early-life...
Abstract Mast cells (MCs) play critical roles in the establishment of allergic diseases. We recently demonstrated an unexpected, proinflammatory role for IL-10 regulating MC responses. enhanced activation and promoted IgE-dependent responses during food allergy. However, whether effects on MCs extend to IgE-independent stimuli is not clear. Herein, we demonstrate that plays a driving IL-33-mediated stimulation significantly expansion, ST2 expression, IL-6 IL-13 production, degranulation...
Abstract Immune regulation by epigenetic mechanisms has become an intense area of research. The concept that our immune system responses are influenced environmental factors including microorganisms, pollution, and pathogen exposure is important as a backdrop for understanding disease progression. We previously observed upregulation KDM5B, H3K4 demethylase, following RSV infection DCs regulates key innate cytokines, Type I IFN TNF. As tri-methylation activating mark, this demethylase should...
Abstract The mechanisms underlying how Respiratory Syncytial Virus (RSV) subverts dendritic cell (DC) immune function to enhance pathology are not well defined, but we have recently shown RSV alters DC metabolism. Analysis of Reverse Phase Protein Array (RPPA) data, which identifies phosphorylated cytoplasmic proteins (>400 in the analysis), revealed one most differentially activated signaling molecules, PARP1, had increased activation (phosphorylation) infected DC. PARP1 is a DNA...
Abstract Mast cells are highly versatile that perform a variety of functions depending on the immune trigger, context activation and cytokine stimulus. Antigen-mediated mast cell responses regulated by transcriptional processes result in induction numerous genes contributing to function. Recently, we also showed exposure dietary agents with known epigenetic actions such as curcumin can suppress cell-mediated food allergy, suggesting vivo may be epigenetically regulated. To further assess...
Abstract We recently demonstrated an unexpected, proinflammatory role for the pleiotropic cytokine, IL-10, in promoting mast cell (MC) responses during food allergy. IL-10 enhanced MC proliferation and survival promoted IgE-dependent to enteric ovalbumin (OVA) challenge. However, whether effects of on MCs extend beyond IgE-mediated signaling is not clear. To determine can prime activation mediated by IgE-independent stimuli, we assessed rIL-10 IL-33-stimulated bone marrow-derived (BMMCs)...