A5000122117- Bone Metabolism and Diseases
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Bone health and treatments
- Erythrocyte Function and Pathophysiology
- Systemic Lupus Erythematosus Research
- Bone health and osteoporosis research
- Connective tissue disorders research
- Bone fractures and treatments
- Orthopedic Surgery and Rehabilitation
- Virus-based gene therapy research
- Cellular transport and secretion
- Genomics and Phylogenetic Studies
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Phagocytosis and Immune Regulation
- Bacteriophages and microbial interactions
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Bone Tissue Engineering Materials
- Orthopaedic implants and arthroplasty
- Heat shock proteins research
- S100 Proteins and Annexins
Hospital for Special Surgery
2024
New York University
2017-2022
NYU Langone Health
2019-2022
VA NY Harbor Healthcare System
2017-2022
City College of New York
2016-2021
Southern California Clinical and Translational Science Institute
2019
New York University Langone Orthopedic Hospital
2017-2018
Hebrew University of Jerusalem
2011
ABSTRACT Osteocyte apoptosis is essential to activate bone remodeling in response fatigue microdamage and estrogen withdrawal, such that inhibition vivo prevents the onset of osteoclastic resorption. has also been spatially linked resorption owing disuse, but whether plays a similar controlling role unclear. We, therefore, 1) evaluated spatial temporal effects disuse from hindlimb unloading (HLU) on osteocyte apoptosis, receptor activator NF-κB ligand (RANKL) expression, resorption, loss...
Osteocyte processes are an order of magnitude more sensitive to mechanical loading than their cell bodies. The mechanisms underlying this remarkable mechanosensitivity not clear, but may be related the infrequent α
Elucidating the effects of mechanical stimulation on bone repair is crucial for optimization healing process. Specifically, regulatory role that loading exerts osteogenic stem cell pool and vascular morphology during incompletely understood. Because dynamic has been shown to enhance osteogenesis repair, we hypothesized induces expansion osteoprogenitor population within a defect, leading an increased presence cells. We further process regulates collagen matrix spatial interactions between...
The autoimmune disease lupus erythematosus (lupus) is characterized by photosensitivity, where even ambient ultraviolet radiation (UVR) exposure can lead to development of inflammatory skin lesions. We have previously shown that Langerhans cells (LCs) limit keratinocyte apoptosis and photosensitivity via a disintegrin metalloprotease 17 (ADAM17)-mediated release epidermal growth factor receptor (EGFR) ligands LC ADAM17 sheddase activity reduced in lupus. Here, we sought understand how the...
Aging is associated with significant bone loss and increased fracture risk, which has been attributed to a diminished response anabolic mechanical loading. In adults, skeletal progenitors proliferate differentiate into bone-forming osteoblasts in increasing stimuli, though the effects of aging on this are not well-understood. Here we show that both adult aged mice exhibit load-induced periosteal formation, significantly attenuated age. We also acute loading involves expansion Sca-1+Prrx1+...
Abstract Mechanical loading is an important aspect of post-surgical care. The timing load application relative to the injury event thought differentially regulate repair depending on stage healing. Here, we show using a novel mechanobiological model cortical defect that daily (5 N peak load, 2 Hz, 60 cycles, 4 consecutive days) during hematoma consolidation and inflammation disrupts site activates cartilage formation periosteal surface adjacent defect. We also matrix deposition phase...
OBJECTIVES/SPECIFIC AIMS: Our aim is to test whether osteocyte-specific CXCL12 expression critical exercise-driven bone formation. METHODS/STUDY POPULATION: All procedures were approved by the NEW YORK UNIVERSITY Institutional Animal Care and Use Committee. We generated male female mice in which was deleted from OCYs (CXCL12ΔOCY) crossing floxed 10kb DMP1-Cre transgenic (gifts Drs. Geoffrey Gurtner Lynda Bonewald, respectively). The has been shown be robustly expressed odontoblasts OCYs,...
Delivering the parathyroid hormone (PTH) gene has been attempted preclinically in a handful of studies, but delivering full-length PTH (1–84) using adeno-associated viral (AAV) vectors not. Given difficulty achieving therapeutic levels secreted proteins therapy, this study seeks to determine feasibility doing so with PTH. An AAV vector was used deliver human driven by strong promoter. We demonstrate ability secrete from various cell types vitro. secretion hepatocytes measured over time and...
Abstract Increased physical loading of the skeleton activates new bone formation ensuring its ability to meet mechanical demands over time; however, capacity respond stimulation diminishes with age. Osteocytes, cells embedded and dispersed throughout mineralized matrix, are master regulators mechanoadaptation through recruitment bone-forming cells, osteoblasts, via signaling osteoprogenitors located on surfaces. We previously demonstrated that in vivo vitro significantly upregulated...