A5042465522- Mycobacterium research and diagnosis
- Immune cells in cancer
- Tuberculosis Research and Epidemiology
- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- interferon and immune responses
- Diabetes and associated disorders
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Cancer Research and Treatments
- Allergic Rhinitis and Sensitization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Toxoplasma gondii Research Studies
- Asthma and respiratory diseases
- Liver Disease Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Blood Coagulation and Thrombosis Mechanisms
- Fungal Infections and Studies
- Kawasaki Disease and Coronary Complications
- RNA modifications and cancer
- Marine Toxins and Detection Methods
- Amino Acid Enzymes and Metabolism
- Pneumocystis jirovecii pneumonia detection and treatment
University of Cincinnati Medical Center
2019-2022
Cincinnati Children's Hospital Medical Center
2017-2022
University of Cincinnati
2017-2021
Miami University
2015-2018
Immunonutrition as a therapeutic approach is rapidly gaining interest in the fight against infection. Targeting l-arginine metabolism intriguing, considering this amino acid substrate for antimicrobial NO production by macrophages. The importance of during infection supported finding that inhibiting its synthesis from precursor l-citrulline blunts host defense. During first few weeks following pulmonary mycobacterial infection, we found drastic increase lung, even though serum concentrations...
Activation, recruitment, and effector function of T lymphocytes are essential for control mycobacterial infection. These processes tightly regulated in cells by the availability l-arginine within microenvironment. In turn, infection dampens cell responsiveness through arginase induction myeloid cells, promoting sequestration local milieu. Here, we show can replenish intracellular metabolism l-citrulline to mediate inflammatory function, allowing anti-mycobacterial overcome arginase-mediated...
Macrophages are indispensable immune cells tasked at eliminating intracellular pathogens. Mycobacterium tuberculosis ( Mtb ), one of the most virulent bacterial pathogens known to man, infects and resides within macrophages. While macrophages can be provoked by extracellular stimuli inhibit kill bacilli, these host defense mechanisms blocked limiting nutritional metabolites, such as amino acids. The acid L-arginine has been well described enhance function, especially in context driving...
Clinical and experimental analyses have identified a central role for IgE/FcεRI/mast cells in promoting IgE-mediated anaphylaxis. Recent data from human studies suggest that bacterial infections can alter susceptibility to
High mobility group AT-hook 1 (HMGA1) protein is an oncogenic architectural transcription factor that plays essential role in early development, but it also implicated many human cancers. Elevated levels of HMGA1 cancer cells cause misregulation gene expression and are associated with increased cell proliferation chemotherapy resistance. We have devised a strategy using engineered viruses to deliver decoy hyper binding sites for the nucleus goal sequestering excess at due competition....
Hypoxia-inducible factor 1α (HIF-1α) regulates the immunometabolic phenotype of macrophages, including orchestration inflammatory and antimicrobial processes. Macrophages deficient in HIF-1α produce excessive quantities anti-inflammatory cytokine interleukin 10 (IL-10) during infection with intracellular fungal pathogen Histoplasma capsulatum (R. A. Fecher, M. C. Horwath, D. Friedrich, J. Rupp, G. S. Deepe, J Immunol 197:565-579, 2016, https://doi.org/10.4049/jimmunol.1600342). Thus,...
The cyanobacterium species Microcystis aeruginosa produces microcystin and an array of diverse metabolites believed responsible for their toxicity and/or immunogenicity. Previously, chronic rhinitis patients were demonstrated to elicit a specific IgE response nontoxic strains M. by skin-prick testing, indicating that cyanobacteria allergenicity resides in non-toxin-producing component the organism.We sought identify characterize peptide(s) allergic sensitization susceptible individuals, we...
Metabolomics analyses suggest changes in amino acid abundance, particularly l-arginine (L-ARG), occur patients with tuberculosis. Immune cells require L-ARG to fuel effector functions following infection. We have previously described an synthesis pathway immune cells; however, its role APCs has yet be uncovered. Using a coculture system mycobacterial-specific CD4
Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis, is responsible for over 1 million deaths each year. Mycobacteria-infected dendritic cells (DCs) migrate to the lymph node initiate adaptive immune priming, which vital antimycobacterial immunity. This response intimately tied nutrient availability – especially amino acid L-arginine (L-ARG), metabolism of altered in TB patients. We have characterized a pathway utilized synthesize L-ARG. Loss L-ARG synthesis CD11c+ cells,...