A5084753906- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Anaerobic Digestion and Biogas Production
- Microbial Fuel Cells and Bioremediation
- Membrane-based Ion Separation Techniques
- Protist diversity and phylogeny
- Geological and Geophysical Studies
- Hydrocarbon exploration and reservoir analysis
- Geochemistry and Elemental Analysis
- Corrosion Behavior and Inhibition
- Biofuel production and bioconversion
- Geological formations and processes
- Metalloenzymes and iron-sulfur proteins
Wuhan University of Technology
2022-2024
Centre National de la Recherche Scientifique
2014-2022
Institut de Recherche pour le Développement
2014-2022
Université de Toulon
2014-2022
Institut Méditerranéen d’Océanologie
2013-2021
Aix-Marseille Université
2014-2015
The shallow submarine hydrothermal field of the Prony Bay (New Caledonia) discharges hydrogen- and methane-rich fluids with low salinity, temperature (< 40°C) high pH (11) produced by serpentinization reactions ultramafic basement into lagoon seawater. They are responsible for formation carbonate chimneys at seafloor. Capillary electrophoresis single-strand conformation polymorphism fingerprinting, quantitative polymerase chain reaction sequence analysis 16S rRNA genes revealed changes in...
Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A dissimilatory sulfite subunit B genes. Methanosarcinales (Euryarchaeota) Thaumarchaea main archaeal members. The...
A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3bT, grew at temperatures 30 to 43 °C (optimum 37 °C) and pH between 7.8 10.1 9.5). Added NaCl not required for growth 0-0.2 %, w/v), but tolerated up 4 %. Yeast extract growth. Strain 3bT utilized crotonate, lactate pyruvate, sugars. Crotonate dismutated acetate butyrate. Lactate disproportionated...
High amounts of hydrogen are emitted in the serpentinite-hosted hydrothermal field Prony Bay (PHF, New Caledonia), where high-pH (~11), low-temperature (< 40°C), and low-salinity fluids discharged both intertidal shallow submarine environments. In this study, we investigated diversity distribution potentially hydrogen-producing bacteria hyperalkaline springs by using metagenomic analyses different PCR-amplified DNA sequencing methods. The retrieved sequences hydA genes, encoding catalytic...
The southeastern part of New Caledonia main island (Grande Terre) is the location a large ophiolitic formation that hosts several hyperalkaline springs discharging high pH (∼11) and warm (<40°C) fluids enriched in methane (CH4) hydrogen (H2). These waters are produced by serpentinization ultrabasic rock formations. Molecular surveys had previously revealed prokaryotic diversity some these Caledonian springs, especially from submarine chimneys Prony Bay hydrothermal field. Here we investigate...
An anaerobic, hydrogenotrophic methane-producing archaeon was isolated from an alkaline thermal spring (42 °C, pH 9.0) in New Caledonia. This methanogen, designated strain CAN T , is alkaliphilic, thermotolerant, with Gram-positive staining non-motile cells. Strain grows autotrophically using hydrogen exclusively as energy source and carbon dioxide the sole (without requirement of yeast extract or other organic compounds). It at 20–45 °C (optimum, 45 °C) 7.3–9.7 9.0). NaCl not required for...
(1) Background: The geothermal spring of La Crouen (New Caledonia) discharges warm (42 °C) alkaline water (pH~9) enriched in dissolved nitrogen with traces methane, but its microbial diversity has not yet been studied. (2) Methods: Cultivation-dependent and -independent methods (e.g., Illumina sequencing quantitative PCR based on 16S rRNA gene) were used to describe the prokaryotic this spring. (3) Results: Prokaryotes mainly represented by Proteobacteria (57% average), followed...