A5011519344- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Anaerobic Digestion and Biogas Production
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Arsenic contamination and mitigation
- Heavy metals in environment
- Microbial Fuel Cells and Bioremediation
- Hydrocarbon exploration and reservoir analysis
- Geological and Geochemical Analysis
- Mercury impact and mitigation studies
- Chromium effects and bioremediation
- Marine and coastal ecosystems
- Mine drainage and remediation techniques
- Wastewater Treatment and Nitrogen Removal
- Geological and Geophysical Studies
- earthquake and tectonic studies
- Protist diversity and phylogeny
- Groundwater and Isotope Geochemistry
- Microbial bioremediation and biosurfactants
- Geology and Paleoclimatology Research
- Paleontology and Stratigraphy of Fossils
- Archaeology and ancient environmental studies
- Geochemistry and Elemental Analysis
- Algal biology and biofuel production
Institut de Recherche pour le Développement
2014-2023
Centre National de la Recherche Scientifique
2010-2023
Institut Méditerranéen d’Océanologie
2014-2023
Université de Toulon
2014-2023
Aix-Marseille Université
2012-2022
Centre of Biotechnology of Sfax
2017-2018
International Ocean Discovery Program
2017
Laboratoire de Biotechnologie de l'Environnement
2011-2014
Bureau de Recherches Géologiques et Minières
2008-2011
Université de Lorraine
2008-2010
A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis AoxB protein sequences showed variable conservation levels and highlighted essential amino acids structural motifs. phylogeny pure strains well-discriminated taxonomic groups similar 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, Gammaproteobacteria-related were...
Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure aoxB-carrying bacterial population, composed members Alpha-, Beta- Gammaproteobacteria, depending on arsenic (As) E(h) levels Upper Isle River Basin waters. The highest gene densities found most As-polluted oxic surface waters but without any significant...
Abstract. The terrestrial hyperalkaline springs of Prony Bay (southern lagoon, New Caledonia) have been known since the nineteenth century, but a recent high-resolution bathymetric survey seafloor has revealed existence numerous submarine structures similar to well-known Aiguille de Prony, which are also location high-pH fluid discharge into lagoon. During HYDROPRONY cruise (28 October 13 November 2011), samples waters, gases and concretions were collected by scuba divers at underwater...
The shallow submarine hydrothermal field of the Prony Bay (New Caledonia) discharges hydrogen- and methane-rich fluids with low salinity, temperature (< 40°C) high pH (11) produced by serpentinization reactions ultramafic basement into lagoon seawater. They are responsible for formation carbonate chimneys at seafloor. Capillary electrophoresis single-strand conformation polymorphism fingerprinting, quantitative polymerase chain reaction sequence analysis 16S rRNA genes revealed changes in...
Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A dissimilatory sulfite subunit B genes. Methanosarcinales (Euryarchaeota) Thaumarchaea main archaeal members. The...
A novel anaerobic bacterial strain, ST07-YET, was isolated from a carbonate chimney of the Prony Hydrothermal Field (PHF) in New Caledonia. Cells were Gram-stain-positive, straight rods (0.7-0.8 × 3.0-5.0 μm) and motile by means lateral flagella. Strain ST07-YET mesophilic (optimum 35 °C), moderately alkaliphilic halotolerant pH 8.7 5 g l- 1 NaCl). Elemental sulfur, sulfate, thiosulfate, sulfite, nitrate nitrite not used as terminal electron acceptors. Yeast extract, peptone, tryptone,...
To investigate the alkane-hydroxylating system of isolate SP2B, closely related to Rhodococcus ruber DSM 43338(T) and uncharacterized so far for its alkane degradation genes.Although SP2B reference strain can grow on by-products from hexane degradation, type R. was unable, unlike isolate, use short-chain alkanes, as assessed by gas chromatography. Using PCR with specific or degenerated primers, inverse Southern blot, two hydroxylase encoding genes (alkB) were detected in both bacteria, which...
Despite their potential importance as analogs of primitive microbial metabolisms, the knowledge structure and functioning deep ecosystems associated with serpentinizing environments is hampered by lack accessibility to relevant systems. These hyperalkaline are depleted in dissolved inorganic carbon (DIC), making sources assimilation pathways highly enigmatic. The Prony Bay Hydrothermal Field (PHF) an active serpentinization site where, similar Lost City (Mid-Atlantic Ridge), high-pH fluids...
Both industrial effluent discharge and the resuspension of contaminated marine sediments are important sources trace metals in seawater which potentially affect ecosystems. The aim this study was to evaluate impact wastewaters having acidic pH (2–3) containing on microbial diversity coastal ecosystem Gulf Gabès (Tunisia, southern Mediterranean Sea) subjected events sediments. Four elements (As, Cd, U V) were monitored during 10-day sediment experiments. highest enrichment dissolved phase...
Rock-hosted subseafloor habitats are very challenging for life, and current knowledge about microorganisms inhabiting such lithic environments is still limited. This study explored the cultivable microbial diversity in anaerobic enrichment cultures from cores recovered during International Ocean Discovery Program (IODP) Expedition 357 Atlantis Massif (Mid-Atlantic Ridge, 30°N). 16S rRNA gene survey of grown at 10-25°C pH 8.5 showed that Firmicutes Proteobacteria were generally dominant....