A5001544428- Wastewater Treatment and Nitrogen Removal
- Parasitic Infections and Diagnostics
- Fecal contamination and water quality
- Microplastics and Plastic Pollution
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Community Ecology and Physiology
- Water Quality Monitoring and Analysis
- Vibrio bacteria research studies
- Membrane Separation Technologies
- Recycling and Waste Management Techniques
- Water Treatment and Disinfection
- Microbial Fuel Cells and Bioremediation
- Legionella and Acanthamoeba research
- Toxoplasma gondii Research Studies
- Pesticide and Herbicide Environmental Studies
- Graphene and Nanomaterials Applications
- Aquaculture disease management and microbiota
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Protist diversity and phylogeny
- Wastewater Treatment and Reuse
- Amoebic Infections and Treatments
- Child Nutrition and Water Access
- Environmental Toxicology and Ecotoxicology
- Innovation, Sustainability, Human-Machine Systems
Universitat Politècnica de València
2014-2024
Hospital Universitari de Santa Maria
2008
Hospital de Santa Maria
2008
Polytechnic University of Puerto Rico
1996-1998
Understanding waterborne protozoan parasites (WPPs) diversity has important implications in public health. In this study, we evaluated a NGS-based method as detection approach to identify simultaneously most WPPs using 18S rRNA high-throughput sequencing. A set of primers target the V4 region such Cryptosporidium spp., Giardia sp., Blastocystis Entamoeba spp, Toxoplasma sp. and free-living amoebae (FLA) was designed. order optimize PCR conditions before sequencing, both mock community with...
This study involved collaboration between three centres with expertise in viruses, bacteria and protozoa. The focus of the research was dissemination removal pathogens faecal indicators two sewage treatment plants (STP1 STP2) using tertiary treatments. Samples were collected over a period five months through processes. Analysis samples revealed that not efficient at removing tested during study. From entry point (raw sewage) to effluent level (tertiary water), experimental results showed...
In this study, the use of skimmed milk flocculation (SMF) to simultaneously concentrate viruses, bacteria and protozoa was evaluated. We selected strains faecal indicator pathogens, such as Escherichia coli Helicobacter pylori. The viruses were adenovirus (HAdV 35), rotavirus (RoV SA-11), bacteriophage MS2 bovine viral diarrhoea virus (BVDV). tested Acanthamoeba, Giardia Cryptosporidium. mean recoveries with q(RT)PCR 66% 24% (MS2), 28% 15% (BVDV), 60% (E. coli), 30% (H. pylori) 21%...
In this study, the presence of Acanthamoeba spp., Blastocystis sp., Cryptosporidium Cyclospora cayetanensis, Entamoeba histolytica, Giardia Toxoplasma gondii and Vermamoeba vermiformis was assessed in organic leafy green vegetables (lettuce, spinach, cabbage) fruits (strawberry), which are usually consumed raw. A total 110 samples were collected Valencia (Spain). Protozoa concentrated before detection by immunofluorescence (Cryptosporidium spp. sp.) or real-time qPCR (Acanthamoeba C. E. T....
This study compared the performance of LMX® broth (LMX), Chromocult Coliform® agar (CC) and Coliform plus cefsulodin (10 μg ml−1) (CC-CFS), with standard methods multiple tube fermentation (MTF), for enumeration total coliforms Escherichia coli from marine recreational waters. LMX CC are two media designed to concurrently detect coliform (TC) bacteria E. by specific action β-galactosidase (total coliforms) β-glucuronidase (E. coli). Overall results TC test showed that LMX, MTF recovered...
The presence of Cryptosporidium and Giardia in waste water is a main concern because reuse for irrigation can jeopardize human health. Spanish Legislation does not oblige to analyze the both pathogens reused irrigation. Therefore, objective this paper determine influence wastewater treatment increase consumer safety margin relation leafy green vegetables. With aim mind, total 108 samples from raw (influent) treated (effluent) three plants Spain were analysed according USEPA Method 1623....
In this work, the influence of bisphenol A (BPA) on biological wastewater treatment was studied. For it, two sequencing batch reactors (SBRs) were operated for three months. Both SBRs fed with synthetic (SW), adding 1 mg·L−1 BPA into feed reactor SBR-BPA, while other one without as a control (SBR–B). addition, experiments performed adapted and non-adapted activated sludge, simulating reaction step to determine pathways removal. Results showed that adsorption biodegradation only significant...
ABSTRACT This study compared the performance of a commercial chromogenic medium, CHROMagarECC (CECC), and CECC supplemented with sodium pyruvate (CECCP) membrane filtration lauryl sulfate-based medium (mLSA) for enumeration Escherichia coli non- E. thermotolerant coliforms (KEC). To establish that we could recover maximum KEC population, two incubation temperature regimens, 41 44.5°C. Statistical analysis by Fisher test data did not demonstrate any statistically significant differences ( P =...
The occurrence of microplastics (MPs) in wastewater has been studied the last years. high efficiency their removal from is linked to transfer sludge. In this work, effect high-density polyethylene (HDPE) on aerobic digestion was evaluated and these MPs were monitored, characterizing them by three different techniques. Two parallel batch digesters monitored. AD-Control (meaning Aerobic Digester) operated as a reference, with no external HDPE particles, whereas polymeric fragments introduced...
Treated sludge from wastewater treatment plants (WWTPs) is commonly used in agriculture as fertilizers and to amend soils. The most significant health hazard for sewage relates the wide range of pathogenic microorganisms such protozoa parasites.The objective this study was collect quantitative data on Cryptosporidium oocysts Giardia cysts treated facilities Spain. Sludge five WWTPs with different stabilization processes has been analysed presence raw after treatment. A composting plant (CP)...
Two extraction procedures were examined, and it was found that DNA recovered from Campylobacter jejuni lysed by the cetyltrimethylammonium bromide (CTAB) method more suitable for use as a PCR template than released boiling method. The region targeted amplification 1.73-kb portion of flagellin A gene C. jejuni. detection limit lower 30 cells per 100 ml in artificially contaminated waters. assay conventional culturing had same sensitivity, but results technique available within 48 h so...
Abstract A new medium, Chromocult Coliform® Agar (CC agar) developed by E. Merck AG (Darmstadt, Germany) was compared with the Standard Methods, membrane filtration fecal coliform (mFC) medium for detection and enumeration. In CC agar, non‐E. coli, coliforms (Klebsiella, Enterobacter, Citrobacter,), (KEC) were identified production of a salmon to red colour from p‐galactosidase (LAC) cleavage substrate Salmon‐GAL, while colonies detected blue colour, produced X‐glucuronide β‐ glucuronidase...
The conservation and preservation of aquatic ecosystems is utmost importance due to the high diversity density species their complex food network. evaluation potential adverse environmental impact caused by pesticides entering water bodies an important parameter in toxicity. toxicity insecticide, Fenitrothion two its metabolites, 3-methyl-4 nitrophenol 3-methyl-4-nitroanisole, herbicides Thiobencarb Molinate, commonly used rice fields Valencia near protected area lake Albufera, has been...
Cefsulodin was evaluated as a potential selective agent for aeromonads. Resistance of Aeromonas and coliform isolates determined by using standard disk diffusion technique. A total 119 78 strains were isolated. For 102 130 [corrected] (environmental reference strains), the MIC cefsulodin < 8 micrograms/ml. Results tests agar dilution method showed that concentration 10 micrograms/ml or less inhibited growth 96% isolates. In comparison, 81 94 > 32 Because suppresses other...