A5053879123- Wastewater Treatment and Nitrogen Removal
- Parasitic Infections and Diagnostics
- Microplastics and Plastic Pollution
- Fecal contamination and water quality
- Water Treatment and Disinfection
- Microbial Community Ecology and Physiology
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Fuel Cells and Bioremediation
- Legionella and Acanthamoeba research
- Recycling and Waste Management Techniques
- Membrane Separation Technologies
- Viral gastroenteritis research and epidemiology
- Enzyme-mediated dye degradation
- Helicobacter pylori-related gastroenterology studies
- Bacterial biofilms and quorum sensing
- Environmental Toxicology and Ecotoxicology
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Graphene and Nanomaterials Applications
- Parasitic infections in humans and animals
- Immune Response and Inflammation
- Water Quality Monitoring and Analysis
Universitat Politècnica de València
2015-2024
Polytechnic University of Puerto Rico
1996-2005
Hospital Universitario de La Princesa
1993
Universidad Autónoma de Madrid
1992
ABSTRACT The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter Campylobacter strains river water wastewater samples. Both 16S 23S rRNA sequence data were used design specific primers oligonucleotide probes FISH analyses, respectively. In order assess the suitability methods, assays performed on naturally artificially contaminated samples compared with isolation cells selective media. detection range varied between 1 cell/ml...
Understanding waterborne protozoan parasites (WPPs) diversity has important implications in public health. In this study, we evaluated a NGS-based method as detection approach to identify simultaneously most WPPs using 18S rRNA high-throughput sequencing. A set of primers target the V4 region such Cryptosporidium spp., Giardia sp., Blastocystis Entamoeba spp, Toxoplasma sp. and free-living amoebae (FLA) was designed. order optimize PCR conditions before sequencing, both mock community with...
This study involved collaboration between three centres with expertise in viruses, bacteria and protozoa. The focus of the research was dissemination removal pathogens faecal indicators two sewage treatment plants (STP1 STP2) using tertiary treatments. Samples were collected over a period five months through processes. Analysis samples revealed that not efficient at removing tested during study. From entry point (raw sewage) to effluent level (tertiary water), experimental results showed...
In this study, the presence of Acanthamoeba spp., Blastocystis sp., Cryptosporidium Cyclospora cayetanensis, Entamoeba histolytica, Giardia Toxoplasma gondii and Vermamoeba vermiformis was assessed in organic leafy green vegetables (lettuce, spinach, cabbage) fruits (strawberry), which are usually consumed raw. A total 110 samples were collected Valencia (Spain). Protozoa concentrated before detection by immunofluorescence (Cryptosporidium spp. sp.) or real-time qPCR (Acanthamoeba C. E. T....
This study compared the performance of LMX® broth (LMX), Chromocult Coliform® agar (CC) and Coliform plus cefsulodin (10 μg ml−1) (CC-CFS), with standard methods multiple tube fermentation (MTF), for enumeration total coliforms Escherichia coli from marine recreational waters. LMX CC are two media designed to concurrently detect coliform (TC) bacteria E. by specific action β-galactosidase (total coliforms) β-glucuronidase (E. coli). Overall results TC test showed that LMX, MTF recovered...
The presence of Cryptosporidium and Giardia in waste water is a main concern because reuse for irrigation can jeopardize human health. Spanish Legislation does not oblige to analyze the both pathogens reused irrigation. Therefore, objective this paper determine influence wastewater treatment increase consumer safety margin relation leafy green vegetables. With aim mind, total 108 samples from raw (influent) treated (effluent) three plants Spain were analysed according USEPA Method 1623....
In this work, the influence of bisphenol A (BPA) on biological wastewater treatment was studied. For it, two sequencing batch reactors (SBRs) were operated for three months. Both SBRs fed with synthetic (SW), adding 1 mg·L−1 BPA into feed reactor SBR-BPA, while other one without as a control (SBR–B). addition, experiments performed adapted and non-adapted activated sludge, simulating reaction step to determine pathways removal. Results showed that adsorption biodegradation only significant...
ABSTRACT This study compared the performance of a commercial chromogenic medium, CHROMagarECC (CECC), and CECC supplemented with sodium pyruvate (CECCP) membrane filtration lauryl sulfate-based medium (mLSA) for enumeration Escherichia coli non- E. thermotolerant coliforms (KEC). To establish that we could recover maximum KEC population, two incubation temperature regimens, 41 44.5°C. Statistical analysis by Fisher test data did not demonstrate any statistically significant differences ( P =...
Helicobacter pylori is one of the most concerning emerging waterborne pathogens. It has been suggested that it could survive in water inside free-living amoebae (FLA), but nobody studied this relationship environment yet. Thus, we aimed to detect viable H. cells from FLA samples. Sixty-nine wastewater and 31 drinking samples were collected. purified identified by PCR sequencing. For exclusively detecting FLA, exposed sodium hypochlorite assayed specific PMA-qPCR, DVC-FISH culture. detected...
Traditional drinking water treatment plants (DWTP) are not specifically designed for removing microplastics (MPs) from water. Therefore, this emergent contaminant has been identified in sources and final Microfibers plastic-based materials a relevant category of MPs that attracted attention recent studies due to their significant presence environmental samples. This study investigated the microfibers (> 10 µm) raw DWTP located Spain. It was observed studied capable 86% Even with high removal...
We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological changes of Campylobacter coli cells during growth. The conversion from spiral coccoid morphology via intermediary forms C. can be detected with procedure. Our data indicate that young or actively growing are mainly shaped (green-stained cells), but older undergo degenerative change (red-stained...