A5045659358- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Tissue Engineering and Regenerative Medicine
- Blood properties and coagulation
- Advanced Materials and Mechanics
- Bone Tissue Engineering Materials
- Microfluidic and Bio-sensing Technologies
- Skin and Cellular Biology Research
- Elasticity and Material Modeling
- Angiogenesis and VEGF in Cancer
- Diffusion and Search Dynamics
- Microbial Fuel Cells and Bioremediation
- Hydrogels: synthesis, properties, applications
- Stochastic processes and statistical mechanics
- Cancer Cells and Metastasis
- Xenotransplantation and immune response
- Genetics, Bioinformatics, and Biomedical Research
- Diatoms and Algae Research
- Biocrusts and Microbial Ecology
- Cell Image Analysis Techniques
- Theoretical and Computational Physics
- Polymer Surface Interaction Studies
- Orthopaedic implants and arthroplasty
Tel Aviv University
2015-2025
Weizmann Institute of Science
2015
California Institute of Technology
2014-2015
Pasadena City College
2015
Technion – Israel Institute of Technology
2006-2014
Rappaport Family Institute for Research in the Medical Sciences
2007
Rambam Health Care Campus
2007
Transplantation of a tissue-engineered heart muscle represents novel experimental therapeutic paradigm for myocardial diseases. However, this strategy has been hampered by the lack sources human cardiomyocytes and scarce vasculature in ischemic area limiting engraftment survival transplanted muscle. Beyond necessity endothelial capillaries delivery oxygen nutrients to grafted tissue, interactions between cardiomyocyte cells may also play key role promoting cell proliferation. In present...
Simple templating of actuators via micro-topographical patterning (STAMP) enables precisely muscle fibers within engineered tissues, enabling fabrication complex multi-oriented architectures such as a biomimetic iris.
Myocardial regeneration strategies have been hampered by the lack of sources for human cardiomyocytes (CMs) and significant donor cell loss following transplantation. We assessed ability a three-dimensional tissue-engineered vascularized cardiac muscle to engraft in vivo rat heart promote functional vascularization. Human embryonic stem cell-derived CMs alone or with endothelial cells (human umbilical vein cells) fibroblasts (triculture constructs) were seeded onto biodegradable porous...
Biological cells sense and respond to mechanical forces, but how such a mechanosensing proccess takes place in nonlinear inhomogeneous fibrous matrix remains unknown. We show that induce deformation fields propagate over longer range than predicted by linear elasticity. Synthetic, elastic hydrogels used many mechanotransduction studies fail capture this effect. develop microstructural finite element model for fiber network simulate localized deformations induced cells. The captures measured...
Extracellular matrix (ECM) provides structural support for cell growth, attachments and proliferation, which greatly impact fate. Marine macroalgae species Ulva sp. Cladophora were selected their variations, porous fibrous respectively, evaluated as alternative ECM candidates. Decellularization-recellularization approach was used to fabricate seaweed cellulose-based scaffolds in-vitro mammalian growth. Both confirmed nontoxic fibroblasts, indicated by high viability up 40 days in culture....
Physical forces direct the orientation of cell division axis for cells cultured on rigid, two-dimensional (2D) substrates. The extent to which physical regulate in three-dimensional (3D) environments is not known. Here, we combine live-cell imaging with digital volume correlation map 3D matrix displacements and identify sites at apply contractile force as they divide. Dividing embedded fibrous matrices remained anchored by long, thin protrusions. During rounding, released adhesive contacts...
Novel tissue-culture bioreactors employ flow-induced shear stress as a means of mechanical stimulation cells. We developed computational fluid dynamics model the complex three-dimensional (3D) microstructure porous scaffold incubated in direct perfusion bioreactor. Our was designed to predict high shear-stress values within physiological range those naturally sensed by vascular cells (1-10 dyne/cm(2)), and will thereby provide suitable conditions for tissue-engineering experiments. The also...
An experimental technique that images fibers of an extracellular matrix to quantify cell-induced deformations and microstructural changes in three dimensions.
When seeded in fibrous gels, pairs of cells or cell aggregates can induce bands deformed gel, extending to surprisingly long distances the intercellular medium. The formation has been previously shown and studied collagen systems. In this study, we strive further our understanding fundamental mechanical mechanism fibrin, a key element wound healing angiogenesis processes. We embedded fibroblast 3D fibrin monitored band by real-time confocal microscopy. Quantitative dynamic analysis revealed...
Abstract Cells sense, manipulate and respond to their mechanical microenvironment in a plethora of physiological processes, yet the understanding how cells transmit, receive interpret environmental cues communicate with distant is severely limited due lack tools quantitatively infer complex tangle dynamic cell-cell interactions complicated environments. We present computational method systematically quantify long-range force transmission through extracellular matrix (cell-ECM-cell...
Force chains (FCs) are a key determinant of the micromechanical properties and behaviour heterogeneous materials, such as granular systems. However, less is known about FCs in fibrous networks composing extracellular matrix (ECM) biological Using finite-element computational model, we simulated contraction single cell two nearby cells embedded two-dimensional elastic analysed tensile that developed ECM. The role ECM nonlinear elasticity on FC formation was evaluated by considering linear...
Myocardial infarction can lead to irreversible heart failure. In an attempt restore function in the failing heart, tissue‐engineered cardiac constructs be applied repopulate scar tissue with a new pool of contractile cells. Effective engineering viable thick complex tissue‐constructs requires intense vascularization. Furthermore, endothelial–cardiomyocyte crosstalk plays key role mutually enhancing functionality, which further improve construct survival. The ability generate engineered,...
Abstract In vivo, eukaryotic cells are embedded in a matrix environment, where they grow and develop. Generally, this extracellular (ECM) is an anisotropic fibrous structure, through which macromolecules biochemical signaling molecules at the nanometer scale diffuse. The ECM continuously remodeled by cells, via mechanical interactions, lead to potential link between biomechanical cell–cell interactions. Here, it studied how cell‐induced forces applied on impact transport of distant cells. It...
There is a growing need for biomaterial scaffolds that support engineering of soft tissue substitutes featuring structure and mechanical properties similar to those the native tissue. This work introduces new system based on centimeter-long collagen fibers extracted from Sarcophyton corals, wrapped around frames create aligned fiber arrays. The arrays displayed hyperelastic viscoelastic resembled collagenous-rich tissues. Cytotoxicity tests demonstrated were nontoxic fibroblast cells. In...
Abstract Fibrous hydrogels are a key component of soft animal tissues. They support cellular functions and facilitate efficient mechanical communication between cells. Due to their nonlinear properties, fibrous materials display non‐trivial force propagation at the microscale, that is enhanced compared linear‐elastic materials. In body, tissues constantly subjected external loads tense or compress them, modifying micro‐mechanical properties into an anisotropic state. However, it unknown how...