Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities diverse niches. In this study we examined the influence of predominant form cell existence exometabolite production by P. strains on interspecies interactions, vitro. Bacterial numbers E. plankton cultures biofilms compared with their single changed a different way, but were accordance existence. The mass mixed-species biofilm was greater than single-species biofilm. Among biofilms, one “planktonic”...

Current methods of biofilm imaging do not support a differentiated assessment its composition, since it is possible to establish substrate stained with crystal violet, as this dye can form complexes both intracellular and extracellular structures. This approach does adequately assess the anti-biofilm effects drugs, while results studying interaction drugs components ensure their most correct choice. The aim investigation was study possibility applying original modification current method...

The study was carried out to assess the prospects for combined use of leukocyte and cytokine indices in bile duct obstruction different genesis.We have performed a patients with mechanical jaundice, either due tumor (14 persons), or non-tumor genesis (16 persons). Concentrations cytokines (IL-1, IL-4, IL-8, TNFα) were determined blood serum before surgical intervention. total activity endogenous inflammatory mediators estimated as an index (IVA), which calculated using following formula: IVA...

Despite the long history of study, laboratory diagnosis gonococcal infection remains a complex task that does not have clearly regulated effective solution. Aim investigation was to assess species diversity microbiota genital tract men and women with suspected acute (AGGI) using test systems Russian manufacturers. A study samples contents urethra 69 posterior vaginal fornix fluids 33 reproductive age characteristic clinical manifestations presumptive AGGI made. Cultivation carried out...

Introduction. Currently, there is an increasing importance of microbial associations in the pathogenesis genital inflammatory diseases. However, issues deciphering taxonomic affiliation and diagnostic significance bacteria detected this case remain unresolved.The aim study was to review quantitative approach determining etiological role microorganisms andrology.Materials methods. For study, samples ejaculate and/or discharge from urethra 15 men who were infertile marriages, 12 with a...

To date, participation of biogenic polyamines has been studied in details, with respect to regulation microbial gene expression, interrelations between bacteria, development their persistence state. Opportunity use as markers human pathological conditions is being actively evaluated. The aim our study was assess the effect bacterial diamines, i.e., cadaverine and putrescine, upon production key cytokines (IFNγ IL-4) culture mononuclear leukocytes. We leukocytes peripheral venous blood...

At present, there are practically no studies deciphering the mechanisms of possible influence opportunistic pathogens on morphological changes in prostate gland. Many authors doubt that bacteria cause chronic prostatitis. In clinical practice with diseases preference is given to ultrasound examination as a reliable and time-consuming diagnostic test. same time, many note for diagnosis inflammatory reproductive organs, study relevant secrets an actual promising scientific direction.The aim...

Gut microbiota (GM) is a peculiar marker of the macroorganism status, capable responding to age, physiological, dietary, climatic and geographical factors by changing qualitative quantitative compositions. The last decade characterized significant reassessment involvement gut into progression chronic human diseases. This article presents an overview recent experimental clinical data on role in development atherosclerosis cardiovascular An attempt has been made analyze mutual modifying...

В настоящем исследовании показано, что выявление условно патогенных микроорганизмов, адгезированных на сперматозоидах, как правило, сопровождается изменением качественных и количественных характеристик сперматозоидов. Высокую степень обсемененности можно рассматривать триггер выработки антиспермальных антител, которые изменяют качественные количественные характеристики сперматозоидов, их двигательную активность. Бессимптомную бактериоспермию целесообразно учитывать при планировании...

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Nowadays, in the presence of wide diagnostic possibilities, laboratory diagnostics microecological disorders vaginal biotope are often limited to clinical data, microscopic examination results and use a culture method. However, with such complex it is impossible get an answer about functional activity microorganisms. The aim investigation was evaluate information content combined study growth parameters ability produce lactic acid by strains Lactobacillus spp. characterize state microecology...

Introduction. At present, the question of commensal, including opportunistic, microflora participation in infertility development remains debatable. In a number studies, translocation vaginal into endometrial tissue is considered as factor contributing to inflammation development. addition, connection some reproductive losses with persistence certain conditionally pathogenic microorganisms shown. Today, solve this issue, molecular genetic research methods are being actively introduced that...

Objective. Various aspects of the organization and optimization diagnosis treatment, including surgical, in patients with hemorrhoids one most common diseases coloproctology are still subject discussion. Problems postoperative period can be prolonged spasm anal sphincter, severe pain syndrome delayed bleeding.
 Materials methods. We conducted a one-center prospective study 214 patients, undergoing surgical treatment for stage 34 chronic hemorrhoids. The surgery was different combination...

Introduction. At present, the question of commensal, including opportunistic, microflora participation in infertility development remains debatable. In a number studies, translocation vaginal into endometrial tissue is considered as factor contributing to inflammation development. addition, connection some reproductive losses with persistence certain conditionally pathogenic microorganisms shown. Today, solve this issue, molecular genetic research methods are being actively introduced that...

Aim. To assess the potential role of M. osloensis in genital microbiota infertile males and females..Materials methods. Samples from men’s urethra women’s posterior vaginal fornix barren couples reproductive age were examined. Cultivation was carried out using elective culture media with subsequent identification strains by biochemical properties. A metagenomic study 16S ribosomal RNA samples performed on Illumina MiSeq platform Reagent Kits v3 kit (600-Cycle Kit).Results. Metagenomic tracts...

Four polyurethane samples have been synthesized. The structure of the materials has studied by IR ATR spectroscopy. biocompatibility initial was evaluated in vitro hemolysis photometric method. Polyurethane urea SPUM-1 found to be most hemocompatible. Two groups surface modification methods developed, a number with organic moieties and radicals grafted obtained. It that introduction long-chain hydrocarbon an aldehyde group into layer results decrease erythrocyte lysis. At СNaCl = 0,45% lysis...