A5038381565- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Virus-based gene therapy research
- Diabetes and associated disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Animal Genetics and Reproduction
- Diabetes Management and Research
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Botulinum Toxin and Related Neurological Disorders
- Neurological disorders and treatments
- Zebrafish Biomedical Research Applications
- Hereditary Neurological Disorders
- Renal and related cancers
- Retinoids in leukemia and cellular processes
- Skin and Cellular Biology Research
- Cannabis and Cannabinoid Research
- Bioactive Compounds and Antitumor Agents
Royan Institute
2016-2025
Academic Center for Education, Culture and Research
2016-2025
City Of Hope National Medical Center
2025
City of Hope
2023-2025
Beckman Research Institute
2024-2025
Kerman University of Medical Sciences
2024
Tarbiat Modares University
2011-2017
Shahed University
2010-2011
Direct conversion of somatic cells into neural stem (NSCs) by defined factors holds great promise for mechanistic studies, drug screening, and potential cell therapies different neurodegenerative diseases. Here, we report that a single zinc-finger transcription factor, Zfp521, is sufficient direct human fibroblasts long-term self-renewable multipotent NSCs. In vitro, Zfp521-induced NSCs maintained their characteristics in the absence exogenous factor expression exhibited morphological,...
Nanocarriers hold great potential for engineering immune cells based on their performance (efficiency, safety and specificity). This review focuses different stages of the transfection process some important challenges.
Polymeric nanocarrier-mediated T cell transfection involves polymer synthesis, isolation and activation, with nanocarriers, evaluation of uptake gene delivery efficiency.
Chimeric antigen receptor (CAR) T-cell therapy is one of the cancer treatment modalities that has recently shown promising results in treating hematopoietic malignancies. However, obstacles need to be addressed solid tumors on-target and off-tumor cytotoxicity due lack specific tumor antigens with low expression healthy cells. Placental alkaline phosphatase (PLAP) a shared placenta- tumor-associated (TAA) expressed ovarian, cervical, colorectal, prostate cancers negligible normal In this...
CRISPR/Cas9 technology provides a powerful tool for targeted modification of genomes. In this system, donor DNA harboring two flanking homology arms is mostly used insertion long exogenous DNA. Here, we introduced an alternative design the by incorporation single short arm into circular plasmid.In experimental study, was applied along with guide RNA (sgRNA) specific to region, and either Cas9 or its mutant nickase variant (Cas9n). Using Pdx1 gene as target locus functionality system...
Poly (β-amino Ester) nanocarriers show promise for gene therapy, but their effectiveness can be limited by the environment within body. This study aims to understand how common cell culture media components affect optimized PBAE nanocarrier performance in delivery.
Background: Diabetes is a major worldwide health problem. It widely accepted that the beta cell mass decreases in type I diabetes (T1D). Accordingly, regeneration promising approach to increase T1D patients. However, underlying mechanisms of have yet be elucidated. One avenue create relevant animal model explore molecular and cellular regeneration. The zebrafish can considered studies because pancreas structure gene expression pattern are highly conserved between human zebrafish. Materials...
Development of potent new anti-Shigella agents for rapid and specific detection treatment is of great importance. Aptamers, nucleic acid oligomers capable binding to a wide range of non-nucleic targets, may be value this purpose. In the present study, we used Systematic Evolution Ligands by Exponential enrichment (SELEX) process select DNA aptamers that bind whole S. sonnei cells. The resulting aptamers exhibited specificity in only to Five unique sequences were isolated from aptamer...
Regulatory T (Treg ) cell therapy is a promising approach for immune tolerance induction in autoimmunity conditions and cell/organ transplantations. Insufficient isolation yields impurity during downstream processes Treg instability after adoptive transfer inflammatory are major limitations to therapy, indicate the importance of seeking valid, reliable method de-novo generation Tregs . In this research, we evaluated -like cells obtained from different differentiation protocols terms their...