A5012165548- Retinal Development and Disorders
- Fish biology, ecology, and behavior
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Diseases and Treatments
- Mitochondrial Function and Pathology
- Zebrafish Biomedical Research Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Ichthyology and Marine Biology
- Genetics, Aging, and Longevity in Model Organisms
- Endoplasmic Reticulum Stress and Disease
- Glaucoma and retinal disorders
- Calpain Protease Function and Regulation
- Aquaculture Nutrition and Growth
- Immune cells in cancer
- Primate Behavior and Ecology
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Bat Biology and Ecology Studies
- 3D Printing in Biomedical Research
- Axon Guidance and Neuronal Signaling
- Telomeres, Telomerase, and Senescence
- Retinal and Optic Conditions
- Marine animal studies overview
Allen Institute for Brain Science
2022-2024
KU Leuven
2021-2024
VIB-KU Leuven Center for Brain & Disease Research
2021-2024
Abstract Glaucoma is a disease associated with the loss of retinal ganglion cells (RGCs), and remains one primary causes blindness worldwide. Major research efforts are presently directed towards understanding pathogenesis development new therapies, help rodent models as an important preclinical tool. The ultimate goal reaching neuroprotection RGCs, which requires tool to reliably quantify RGC survival. Hence, we demonstrate novel deep learning pipeline that enables fully automated...
Worldwide, people are getting older, and this prolonged lifespan unfortunately also results in an increased prevalence of age-related neurodegenerative diseases, contributing to a diminished life quality elderly. Age-associated neuropathies typically include diseases leading dementia (Alzheimer's Parkinson's disease), as well eye such glaucoma macular degeneration. Despite many research attempts aiming unravel aging processes their involvement neurodegeneration functional decline, achieving...
Abstract Age‐related vision loss caused by retinal neurodegenerative pathologies is becoming more prevalent in our ageing society. To understand the physiological and molecular impact of on homeostasis, we used short‐lived African turquoise killifish, a model known to naturally develop central nervous system (CNS) hallmarks loss. Bulk single‐cell RNA‐sequencing (scRNAseq) three age groups (6‐, 12‐, 18‐week‐old) identified transcriptional fingerprints killifish retina, unveiling pathways also...
Successful axonal regeneration following injury requires the effective allocation of energy. How axons withstand initial disruption in mitochondrial energy production caused by and subsequently initiate regrowth is poorly understood. Transcriptomic data showed increased expression glycolytic genes after optic nerve crush retinal ganglion cells with co-deletion Pten Socs3. Using cultures a multicompartment microfluidic device, we observed enhanced trafficking Socs3 co-deleted neurons. While...
Optic neuropathies comprise a diverse group of disorders that ultimately lead to retinal ganglion cell (RGC) degeneration. Despite varying etiologies, these conditions share conserved pathological progression: axonal damage in the optic nerve triggers progressive RGC Understanding species-specific differences neuronal resilience is critical for identifying key survival mechanisms and potential neuroprotective targets. In this study, we compare densities rates following crush (ONC) three...
Unlike mammals, zebrafish can regrow axons after injury and restore circuit function in the central nervous system (CNS). Mitochondria have been identified as key players this process, but how different metabolic pathways work together to sustain regeneration remains unclear. Using RNA sequencing of adult retinal ganglion cells optic nerve crush injury, we found that oxidative phosphorylation is downregulated during axonal regrowth. Simultaneously, thioredoxin antioxidant was upregulated,...
Abstract The multifaceted nature of neuroinflammation is highlighted by its ability to both aggravate and promote neuronal health. While in mammals retinal ganglion cells (RGCs) are unable regenerate following injury, acute inflammation can induce axonal regrowth. However, the cells, cellular states signalling pathways that drive this inflammation-induced regeneration have remained elusive. Here, we investigated functional significance macrophages during RGC de- regeneration, characterizing...
As the mammalian central nervous system matures, its regenerative ability decreases, leading to incomplete or non-recovery from neurodegenerative diseases and insults that we are increasingly facing in our aging world population. Current neuroregenerative research is largely directed toward identifying molecular cellular players underlie repair, yet it repeatedly ignores context which many of these appear. Using an optic nerve crush model a novel biogerontology model, is, short-living...
Neuroinflammation has been put forward as a mechanism triggering axonal regrowth in the mammalian central nervous system (CNS), yet little is known about underlying cellular and molecular players connecting these two processes. In this study, we provide evidence that MMP2 an essential factor linking inflammation to regeneration by using vivo mouse model of inflammation-induced optic nerve. We show infiltrating myeloid cells abundantly express deficiency results reduced long-distance...
Axonal regeneration in the central nervous system is an energy-intensive process. In contrast to mammals, adult zebrafish can functionally recover from neuronal injury. This raises question of how cope with this high energy demand. We previously showed that zebrafish, subjected optic nerve crush, antagonistic axon-dendrite interplay exists wherein retraction retinal ganglion cell dendrites a prerequisite for effective axonal repair. postulate 'dendrites regeneration' paradigm might be linked...
As modern society is graying, aging research and biogerontology models, in which the process can be studied, are becoming increasingly important. A proper model defined as one that displays many of hallmarks. Here, we provide two different practical approaches—namely, real-time quantitative polymerase chain reaction (RT-qPCR) western blotting—that used to investigate cellular senescence (RT-qPCR for p21 p27 ), altered intercellular communication/inflammaging il-10 , sirt-1 il-6 il-1b il-8...
Optic neuropathies comprise a group of disorders in which the axons retinal ganglion cells (RGCs), projection neurons conveying visual information to brain, are damaged. This results impairment or even blindness, is irreversible as adult mammals lack capacity repair replace injured lost neurons. Despite intensive research, no efficient treatment induce axonal regeneration central nervous system (CNS) available yet. Autophagy, cellular recycling response, was shown repeatedly be elevated...
As the number of elderly individuals is increasing in modern society, need for a relevant gerontology model higher than ever before. Aging can be defined by specific cellular hallmarks, described López-Otín and colleagues, who provided map which used to scavenge aging tissue environment. revealing presence individual hallmarks does not necessarily indicate aging, here we provide different (immuno)histochemical approaches that investigate several hallmarks—namely, genomic damage,...
Unlike mammals, adult zebrafish are able to fully regenerate axons and functionally recover from neuronal damage in the mature central nervous system (CNS). Decades of research have tried identify mechanisms behind their spontaneous regenerative capacity, but exact underlying pathways molecular drivers remain be elucidated. By studying optic nerve injury-induced axonal regrowth retinal ganglion cells (RGCs), we previously reported transient dendritic shrinkage changes distribution morphology...
Loss of vision is a prominent feature aging and considered by many to be the most valuable sense lost. In our graying society, we are increasingly challenged age-related deterioration central nervous system (CNS), as well age-associated neurodegenerative diseases brain injuries, all often affecting visual thus its performance. Here, describe two visually driven behavior assays evaluate performance upon or CNS damage in fast-aging killifish. The first test, optokinetic response (OKR),...
Age-related vision loss caused by retinal neurodegenerative pathologies is becoming more prevalent in our ageing society. To understand the physiological and molecular impact of on homeostasis, we used short-lived African turquoise killifish, a model known to naturally develop central nervous system (CNS) hallmarks loss. Bulk single-cell RNA-sequencing (scRNA-seq) three age groups (6-, 12-, 18-week-old) identified transcriptional fingerprints killifish retina, unveiling pathways also aged...
Wolfram syndrome (WS) is a rare childhood disease characterized by diabetes mellitus, insipidus, blindness, deafness, neurodegeneration and eventually early death, due to autosomal recessive mutations in the WFS1 (and WFS2) gene. While it categorized as neurodegenerative disease, increasingly becoming clear that other cell types besides neurons may be affected contribute pathogenesis. MRI studies patients phenotyping WS rodent models indicate white matter/myelin loss, implicating role for...
Decades of research asserted that the oligodendroglial lineage comprises two cell types: oligodendrocyte precursor cells and oligodendrocytes. However, recent studies employing single-cell RNA sequencing techniques have uncovered novel states, prompting a revision existing terminology. Going forward, should be delineated into five distinct states: cells, committed newly formed oligodendrocytes, myelin-forming mature This new classification system enables deeper understanding oligodendroglia...
In our graying world population, we are increasingly facing brain injuries and age-associated neurodegenerative diseases, which often characterized by axonal pathology. Here, propose the killifish visual/retinotectal system as a model for investigating central nervous repair, more specifically regeneration, in an aging context. We first describe optic nerve crush (ONC) injury paradigm to induce study both de- regeneration of retinal ganglion cells (RGCs) their axons. Subsequently, summarize...
SUMMARY Successful axonal regeneration following injury requires the effective allocation of energy. Mitochondria play a pivotal role, accumulating at tips growing axons to fuel regeneration. However, how withstand initial disruption in mitochondrial energy production caused by injury, and subsequently initiate regrowth is poorly understood. Using retinal cultures multicompartment microfluidic device, we observed increased enhanced trafficking ganglion cells with deletion both Pten Socs3 ....