A5079824959- Fish biology, ecology, and behavior
- Microtubule and mitosis dynamics
- Aquaculture Nutrition and Growth
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Chromosomal and Genetic Variations
- Primate Behavior and Ecology
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Zebrafish Biomedical Research Applications
- Fish Biology and Ecology Studies
- Physiological and biochemical adaptations
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Congenital heart defects research
- Planarian Biology and Electrostimulation
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Ichthyology and Marine Biology
- Retinal Diseases and Treatments
Stanford University
2015-2024
Stony Brook University
2021-2024
Center for Systems Biology
2006-2012
Harvard University
2006-2012
Contractile actin cortex is involved in cell morphogenesis, movement, and cytokinesis, but its organization assembly are poorly understood. During blebbing, the membrane detaches from inflates. As expansion ceases, contractile reassembles under drives bleb retraction. This cycle enabled us to measure temporal sequence of protein recruitment during reassembly explore dependency relationships. Expanding blebs were devoid actin, proteins erythrocytic submembranous cytoskeleton present. When...
The midbody is a transient structure that connects two daughter cells at the end of cytokinesis, with principal function being to localize site abscission, which physically separates cells. Despite its importance, understanding assembly and regulation still limited. Here we describe how structural composition changes during progression throughout cytokinesis explore functional implications these changes. Deriving from midzones, midbodies are organized by set microtubule interacting proteins...
Model organisms are widely used in research as accessible and convenient systems to study a particular area or question biology. Traditionally only handful of have been studied, but modern tools enabling researchers extend the set model include less-studied more unusual systems. This Forum highlights range 'non-model organisms' emerging for tackling questions across whole spectrum biology (and beyond), opportunities challenges, outlook future.
Vertebrates vary in their ability to regenerate, and the genetic mechanisms underlying such disparity remain elusive. Comparative epigenomic profiling single-cell sequencing of two related teleost fish uncovered species-specific evolutionarily conserved genomic responses regeneration. The response revealed several regeneration-responsive enhancers (RREs), including an element upstream inhibin beta A (inhba), a known effector vertebrate This activated expression regenerating transgenic fish,...
Diapause is a state of suspended development that helps organisms survive extreme environments. How diapause protects living largely unknown. Using the African turquoise killifish (Nothobranchius furzeri), we show preserves complex for extremely long periods time without trade-offs subsequent adult growth, fertility, and life span. Transcriptome analyses indicate an active state, with dynamic regulation metabolism organ genes. The most up-regulated genes in include Polycomb members....
During cytokinesis, a specialized set of proteins is recruited to the equatorial region between spindle poles by microtubules and actin filaments, enabling furrow assembly ingression before cell division. We investigate mechanisms underlying regional specialization cytoskeleton in HeLa cells undergoing drug-synchronized monopolar cytokinesis. After forced mitotic exit, initially radially symmetric but undergoes symmetry-breaking reaction that simultaneously polarizes cortex, with...
To achieve mitosis and cytokinesis, microtubules must assemble into distinct structures at different stages of cell division-mitotic spindles to segregate the chromosomes before anaphase midzones keep sister genomes apart guide cleavage furrow after anaphase. This temporal regulation is believed involve Cdk1 kinase, which inactivated in a switch-like way We found that inhibiting Plk1 caused premature assembly cells still metaphase, breaking microtubules. The antiparallel microtubule-bundling...
Mitotic spindles play essential roles in chromosome congression and segregation during mitosis. Aurora A regulates spindle assembly part via phosphorylating human TACC3 on S558, which triggers relocalization to mitotic stabilizes microtubules (MTs). In this study, we identified clathrin heavy chain (CHC) as an adaptor protein recruit S558-phosphorylated onto the mitosis for MT stabilization. CHC binds phospho-S558 its linker domain first repeat. depletion or mutation phospho-TACC3 binding...
Suspended animation states allow organisms to survive extreme environments. The African turquoise killifish has evolved diapause as a form of suspended development complete drought. However, the mechanisms underlying evolution survival are unknown. To understand evolution, we performed integrative multi-omics (gene expression, chromatin accessibility, and lipidomics) in embryos multiple species. We find that by recent remodeling regulatory elements at very ancient gene duplicates (paralogs)...
The African turquoise killifish is an exciting new vertebrate model for aging studies. A significant challenge any organism the control over its diet in space and time. To address this challenge, we created automated networked fish feeding system. Our feeder designed to be open-source, easily transferable, built from widely available components. Compared manual feeding, our system highly precise flexible. As a proof of concept flexibility these feeders, define favorable regimen growth...
Abstract Age‐related vision loss caused by retinal neurodegenerative pathologies is becoming more prevalent in our ageing society. To understand the physiological and molecular impact of on homeostasis, we used short‐lived African turquoise killifish, a model known to naturally develop central nervous system (CNS) hallmarks loss. Bulk single‐cell RNA‐sequencing (scRNAseq) three age groups (6‐, 12‐, 18‐week‐old) identified transcriptional fingerprints killifish retina, unveiling pathways also...
Abstract The successful completion of cytokinesis requires the coordinated activities diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on cell type. biochemical analysis this process is challenging due to its dynamic rapid nature. Here, we systematically compared monopolar bipolar demonstrated a good surrogate for it well-suited system global in mammalian cells. Based this, established...
ABSTRACT Suspended animation states such as hibernation or diapause allow organisms to survive extreme environments. But the mechanisms underlying evolution of these survival are unknown. The African turquoise killifish has evolved a form suspended development complete drought that occurs every year in its habitat. Here we show many gene duplicates – paralogs exhibit specialized expression versus normal killifish. Surprisingly, with evolutionarily very ancient, and they also present even...
Abstract The African turquoise killifish is an exciting new vertebrate model for aging studies. A significant challenge any organism the control over its diet in space and time. To address this challenge, we created automated networked fish feeding system. Our feeder designed to be open-source, easily transferable, built from widely available components. Compared manual feeding, our system highly precise flexible. As a proof-of-concept flexibility of these feeders, define favorable regimen...
Age-related vision loss caused by retinal neurodegenerative pathologies is becoming more prevalent in our ageing society. To understand the physiological and molecular impact of on homeostasis, we used short-lived African turquoise killifish, a model known to naturally develop central nervous system (CNS) hallmarks loss. Bulk single-cell RNA-sequencing (scRNA-seq) three age groups (6-, 12-, 18-week-old) identified transcriptional fingerprints killifish retina, unveiling pathways also aged...