A5047908565- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- T-cell and Retrovirus Studies
- NF-κB Signaling Pathways
- Signaling Pathways in Disease
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- RNA Interference and Gene Delivery
- Synthesis and Characterization of Pyrroles
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Peptidase Inhibition and Analysis
- Circadian rhythm and melatonin
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Protein Degradation and Inhibitors
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
Institute of Biomedical Sciences, Academia Sinica
2013-2024
Taipei Medical University
2017-2024
Academia Sinica
2007-2024
National Health Research Institutes
2001-2022
National Defense Medical Center
2009-2013
Institute of Life Sciences
2013
City Of Hope National Medical Center
2013
Institute of Biomedical Science
2013
Taipei Hospital
2013
National Taiwan University
1997-2011
Adipocyte determination- and differentiation-dependent factor 1 (ADD1), a member of the basic helix-loop-helix (bHLH) family transcription factors, has been associated with both adipocyte differentiation cholesterol homeostasis (in which case it termed SREBP1). Using PCR-amplified binding analysis, we demonstrate that ADD1/SREBP1 dual DNA sequence specificity, to an E-box motif (ATCACGTGA) non-E-box previously shown be important in metabolism, sterol regulatory element (SRE-1; ATCACCCCAC)....
Regulatory sequences involved in the transcriptional induction of rat S14 gene response to increased glucose metabolism hepatocyte were investigated and compared with those liver-type pyruvate kinase (L-PK) gene. The carbohydrate element (ChoRE) was found consist two motifs related consensus binding site for c-myc family transcription factors, CACGTG. These are separated by five base pairs, a similar arrangement that L-PK ChoRE. In its natural context, ChoRE requires novel accessory factor...
The 5‘-flanking region of the S14 gene from -4316 to +18 contains regulatory sequences responsible for activation promoter activity in response elevated carbohydrate metabolism primary hepatocytes. To map these sequences, a series constructs containing various internal deletions sequence were assayed -1601 -1395 was found be essential this response. Comparison L-type pyruvate kinase that has been shown mediate regulation (Thompson, K. S., and Towle, H. C. (1991) J. Biol. Chem. 266,...
The DNA damage response (DDR) involves both the control of repair and signaling to cell cycle checkpoints. Therefore, unraveling underlying mechanisms DDR is important for understanding tumor suppression cellular resistance clastogenic cancer therapeutics. Because likely be influenced by chromatin regulation at sites damage, we investigated role heterochromatin protein 1 (HP1) during process. We monitored double-strand breaks (DSBs) using γH2AX foci marker found that depleting cells HP1...
Mitotic spindles play essential roles in chromosome congression and segregation during mitosis. Aurora A regulates spindle assembly part via phosphorylating human TACC3 on S558, which triggers relocalization to mitotic stabilizes microtubules (MTs). In this study, we identified clathrin heavy chain (CHC) as an adaptor protein recruit S558-phosphorylated onto the mitosis for MT stabilization. CHC binds phospho-S558 its linker domain first repeat. depletion or mutation phospho-TACC3 binding...
A growth hormone (GH)-inducible nuclear factor (GHINF) from rat liver has been purified to near homogeneity. On SDS-polyacrylamide gel electrophoresis and UV-cross-linking, a major band of mass ∼93 kDa minor ∼70 are detected in the fraction. DNase I footprinting using GHINF yields protected region −149/−115 on serine protease inhibitor 2.1 (Spi 2.1) promoter encompassed within response element (GHRE). Mutational analysis demonstrated that binds synergistically two γ-interferon-activated...
Transcription of the 514 gene in primary hepatocytes is stimulated response to increased carbohydrate metabolism.We have demonstrated previously that a 30- base pair (bp) segment from -1457 -1428 element (ChoRE).This contains (5')CACGTG motif essential for control.DNase I footprinting experiments with liver nuclear extract revealed two factors binding within S14 ChoRE.In transient transfection experiments, mutation upstream site between and -1450 did not affect elevated glucose, whereas...
OBJECTIVE. Enterovirus 71 has caused large epidemics of disease, resulting in many fatalities and severe sequelae, Taiwan some other countries. In this study, host genetic factors were investigated to link susceptibility clinical severity enterovirus infections. METHODS. We enrolled 219 case subjects 97 control children. HLA typing was performed with sequence-specific primers, polymorphisms immune-related candidate genes detected polymerase chain reaction, followed by automated gene...
Constitutive NF-κB activation (NF-κBCA) confers survival and proliferation advantages to cancer cells frequently occurs in T/B cell malignancies including adult T leukemia (ATL) caused by human T-cell virus type 1 (HTLV-1). Counterintuitively, NF-κBCA the HTLV-1 transactivator/oncoprotein Tax induces a senescence response, infections culture mostly result or cell-cycle arrest due How senescence, how ATL maintain avert remain unclear. Here we report that increases R-loop accumulation DNA...
We found in the present study that stimulation of A2A adenosine receptor (A2A-R) using an A2A-selective agonist (CGS21680) rescued blockage nerve growth factor (NGF)-induced neurite outgrowth when NGF-evoked MAPK cascade was suppressed by MEK inhibitor (PD98059) or a dominant-negative mutant (dnMAPK). This action A2A-R (designated as A2A-rescue effect) can be blocked two inhibitors protein kinase A (PKA) and absent PKA-deficient PC12 variant. Activation cAMP/PKA pathway forskolin exerted...
p38 mitogen activated protein kinase (MAPK) is essential for T-cell activation. Here we demonstrated that nuclear factor of T cells (NFAT) a direct target MAPK. Inhibition MAPK led to selective inactivation NFAT in cells. We further linked strict requirement activation NFATc. A stimulatory effect on at least four other stages NFATc was found. First, the cascade promoter and induced transcription mRNA. Second, mildly increased mRNA stability Third, enhanced translation Fourth, promoted...
Human herpesvirus 8 (HHV8) is the primary viral etiologic agent in Kaposi's sarcoma (KS). However, individuals dually infected with both HHV8 and human immunodeficiency virus type 1 (HIV-1) show an enhanced prevalence of KS when compared those singularly HHV8. Host immune suppression conferred by HIV infection cannot wholly explain this increased presentation KS. To better understand how HIV-1 might interact directly pathogenesis KS, we queried for potential regulatory interactions between...
ABSTRACT Human immunodeficiency virus type 1 viral protein R (Vpr) is required for pathogenesis and has been implicated in T-cell apoptosis through its activation of caspase 3 9 perturbation mitochondrial membrane potential. To understand better Vpr-mitochondria interaction, we report here the identification antiapoptotic HAX-1 as a novel Vpr target. We show that physically associate with each other. Overexpression cells dislocates from normal residence mitochondria creates mitochondrion...
Human T lymphotropic virus type 1 (HTLV-1) trans-activator/oncoprotein, Tax, impacts a multitude of cellular processes, including I-κB kinase (IKK)/NF-κB signaling, DNA damage repair, and mitosis. These activities Tax have been implicated in the development adult T-cell leukemia (ATL) HTLV-1-infected individuals, but underlying mechanisms remain obscure. IKK its upstream kinase, TGFβ-activated (TAK1), contain ubiquitin-binding subunits, NEMO TAB2/3 respectively, which interact with...
Glioblastoma multiforme ( GBM ) is a highly malignant type of brain tumor found in humans. cells reproduce quickly, and the median survival time for patients after therapy approximately 1 year with high relapse rate. Current therapies diagnostic tools are limited; therefore, we searched more favorable therapeutic target or marker protein both diagnosis. We used mass spectrometry MS analysis to identify ‐associated proteins from human plasma cell cultures. Additional 52 tissues obtained...
Transforming acidic coiled-coil protein 3 (TACC3) was reported to be important for regulating mitotic spindle assembly and chromosome segregation. While the level of TACC3 shown altered during cell cycle progression, molecular mechanism in controlling is unclear. Here, we show that can regulated by Cdh1, a well known activator anaphase-promoting complex/cyclosome. We identified Cdh1 as an interacting partner yeast array screen. Both vitro vivo binding studies indicated form complexes with...