A5062741054- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- RNA Research and Splicing
- HIV/AIDS Research and Interventions
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Nuclear Structure and Function
- Vector-Borne Animal Diseases
- Blood groups and transfusion
- Viral Infections and Vectors
- Reproductive System and Pregnancy
- RNA modifications and cancer
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- RNA regulation and disease
- Bacteriophages and microbial interactions
- interferon and immune responses
- HIV-related health complications and treatments
- Immune cells in cancer
- Blood disorders and treatments
- Signaling Pathways in Disease
- Retinoids in leukemia and cellular processes
Center for Biologics Evaluation and Research
2002-2018
Northwestern Polytechnical University
2011
United States Food and Drug Administration
2009-2011
Institute of Environmental Science and Research
2009
The Centers
2009
Centers for Disease Control and Prevention
2009
University of British Columbia
2009
American Red Cross
2009
Food and Drug Administration
2008
National Institutes of Health
1989-1995
The envelope of the human immunodeficiency virus type 1 (HIV-1) plays a central role in process entry into host cell and cytopathicity for lymphocytes bearing CD4 molecule. Mutations that affect ability glycoprotein to form syncytia + cells can be divided five groups: those decrease binding protein molecule, prevent post-binding fusion reaction, disrupt anchorage membrane, association two subunits glycoprotein, post-translational proteolytic processing precursor protein. These findings...
The art gene product of the human immunodeficiency virus is required for expression virion capsid and envelope proteins. experiments presented here show that sequences located within coding region exert a negative effect on heterologous genes these can be relieved by product. This in env contains regulatory inhibit expression, as well sequence necessary product-dependent relief repression. define cis- trans-acting components system permits differential structural
Expression of high levels the structural proteins human immunodeficiency virus type 1 (HIV-1) requires presence protein encoded by rev open reading frame (Rev) and its associated target sequence CAR (cis anti-repression sequence) which is present in env region viral RNA. Extensive mutagenesis demonstrated that has a complex secondary structure consisting central stem five stem/loops. Disruption any these structures severely impaired Rev response, but many stem/loops contain material was...
A human cDNA library was screened for sequences homologous to the erbA gene of avian erythroblastosis virus (AEV). One such clone, cHerbA-1, used map chromosomal location highly that were found be present on chromosome 17 as judged by Southern blot screening a panel mouse-human hybrid cell lines segregating chromosomes. cHerbA-1 hybridized in situ metaphase chromosomes from normal male subject and female patient with an acute promyelocytic leukemia (APL) having typical t(15;17)...
BACKGROUND: West Nile virus (WNV) transmission by transfusion was documented in 2002. Approximately 80 percent of WNV infections are asymptomatic and 1 develop severe neurological illness. In animals, Langerhans‐dendritic cells support initial viral replication, followed replication lymphoid tissues dissemination to organs possibly the CNS. The cellular tropism infection after particular human blood that sustain remain largely unknown. Whether primary monocyte‐derived macrophages (MDMs)...
ABSTRACT Human immunodeficiency virus type 1 viral protein R (Vpr) is required for pathogenesis and has been implicated in T-cell apoptosis through its activation of caspase 3 9 perturbation mitochondrial membrane potential. To understand better Vpr-mitochondria interaction, we report here the identification antiapoptotic HAX-1 as a novel Vpr target. We show that physically associate with each other. Overexpression cells dislocates from normal residence mitochondria creates mitochondrion...
Natural Killer (NK) cells are a crucial component of the host innate immune system with anti-viral and anti-cancer properties. However, role NK in West Nile virus (WNV) infection is controversial, reported effects ranging from active suppression to no effect at all. It was previously shown that K562-mb15-41BBL (K562D2) cells, which express IL-15 4-1BBL on K562 cell surface, were able expand activate human primary normal peripheral blood mononuclear (PBMC). The expanded tested for their...
As part of a program to develop Dengue virus vaccine which avoids the deleterious effects antibody dependent enhancement (ADE) infection mediated by antibodies structural proteins, we have begun investigate possibility designing vaccines based on non-structural proteins. constructs lack major proteins replicate intracellularly in tissue culture. These replicons are capable prolonged expression for at least seven days genomes lacking can, like other flaviviruses, and express with minimal...
The significance and location of sequence-specific information in the CAR/RRE, target sequence for Rev protein human immunodeficiency virus type 1 (HIV-1), have been controversial. We present here a comprehensive experimental computational approach combining mutational analysis, phylogenetic comparison, thermodynamic structure calculations with systematic strategy distinguishing from secondary structural information. A analog was designed to identical that wild but differs at every position....
Implementation of sensitive screening methods for human immunodeficiency virus (HIV) and hepatitis viruses prompts the question what quantitative risks may result from altered deferral strategies donation blood by men who have had sex with (MSM).Quantitative probabilistic models were developed to assess changes in residual risk transfusion-transmitted HIV B (HBV) associated testing quarantine release errors (QREs) initial year two hypothetical policy scenarios that would allow donations...
Developing T cells are subjected to a gauntlet of tests in the thymus ensure safe and effective cell receptor (TCR) repertoire. β-selection, checkpoint early development, functionality rearranged TCRβ chains. Cells with functional chains receive pre-TCR signals, which promote survival, proliferation, differentiation, maturation TCR. While signals associated mature TCR have been carefully studied, those not well described. Thus, we sought characterize that occur during development. We first...
NK cells are prominent mediators of the immunomodulating and antiangiogenic activity IL-12. However, effect prolonged IL-12 treatment on is unclear. In this study, we observed that initially activates cells, but specifically down-regulates signaling induces cell apoptosis associated with a significant reduction in cytolytic IFN-γ production response to further stimulation. Further results demonstrate stimulation decreases level activated STAT4 protein, critical component, through decreasing...
Correct endoproteolytic maturation of gp160 is essential for the infectivity human immunodeficiency virus type 1. This processing virus-1 envelope protein, gp160, into gp120 and gp41 has been attributed to activity cellular subtilisin-like proprotein convertase furin. The prototypic furin recognition cleavage site Arg-X-Arg/Lys-Arg. Arg-Arg-Arg-Arg-Arg-Arg or longer iterations polyarginine have shown be competitive inhibitors substrate by Here, we tested inhibition productive...
Significant sex specific differences in the progression of HIV/AIDS have been reported. Several studies implicated steroid hormones regulating host factor expression and modulating HIV transmission replication. However, exact mechanism exerted by estrogen progesterone regulation HIV-1 replication is still unclear. Results from current study indicated a dose dependent down monocyte derived macrophages pre-treated with high concentrations or progesterone. To elucidate molecular mechanisms...
Toward the goals of providing an additional vector to add armamentarium available HIV vaccinologists and creating a bivalent vaccine effective against dengue virus HIV, we have attempted create vectors which express non-structural proteins immunogens. Previously reported successful construction replicons lack structural genes necessary for virion release spreading infection in culture but can replicate intracellularly abundantly produce proteins. Here heterologous genetic material from these...
Nucleo-cytoplasmic transport of RNA is one many cellular pathways whose illumination has progressed hand in with understanding retroviral mechanisms. A recent paper Cell reports the involvement an helicase pathway by which HIV exports partially spliced and unspliced out nucleus. This suggests ubiquity helicases export from nucleus, novel mechanistic implications.