A5045872492- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Respiratory viral infections research
- Reproductive tract infections research
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- Biosensors and Analytical Detection
- Animal Disease Management and Epidemiology
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Influenza Virus Research Studies
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Signaling Pathways in Disease
- Syphilis Diagnosis and Treatment
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
Southern Medical University
2016-2025
Nanfang Hospital
2021-2024
University of Chinese Academy of Sciences
2021-2022
Institute of Microbiology
2018
Guangdong Provincial People's Hospital
2018
Center for Biologics Evaluation and Research
2006-2013
United States Food and Drug Administration
2006-2011
New York University
2010
Johns Hopkins University
2010
Lund University
2010
ABSTRACT OBJECTIVE To investigate the impact of temperature and absolute humidity on coronavirus disease 2019 (COVID-19) outbreak. DESIGN Ecological study. SETTING 31 provincial-level regions in mainland China. MAIN OUTCOME MEASURES Data COVID-19 incidence climate between Jan 20 Feb 29, 2020. RESULTS The number new confirm cases China peaked 1, daily were lowest at -10 °C highest 10 °C, while maximum was observed approximately 7 g/m 3 . changed with as decreased when rose. No significant...
Chimeric antigen receptor (CAR) technology has revolutionized cancer treatment, particularly in malignant hematological tumors. Currently, the BCMA-targeted second-generation CAR-T cells have showed impressive efficacy treatment of refractory/relapsed multiple myeloma (R/R MM), but up to 50% relapse remains be addressed urgently. Here we constructed fourth-generation expressing IL-7 and CCL19 (i.e., BCMA-7 × 19 cells), demonstrated that exhibited superior expansion, differentiation,...
Real-world evidence on inactivated COVID-19 vaccines against the highly transmissible B.1.617.2 (Delta) variant of SARS-CoV-2 is limited, leaving an important gap in base about for use by immunization programs.
A group of conserved hydrophobic residues faces the interior coiled-coil-like structure within N-terminal domain human immunodeficiency virus type 1 (HIV-1) capsid protein (CA). It has been suggested that these are important for maintaining stable and functional activity. To investigate this possibility, we constructed two HIV-1 clones, in which Trp23 or Phe40 was changed to Ala. We also a third mutant, D51A, mutation destroys salt bridge between Pro1 Asp51. All three mutants replication...
We evaluated the feasibility of using nanoparticle (NP)-based assays for improving detection sensitivity human immunodeficiency virus type 1 (HIV-1) p24 antigen. One assay that was is a gold NP-based biobarcode amplification (BCA) assay, which can detect HIV-1 antigen at levels as low 0.1 pg/mL. The lower limit an enzyme-linked immunosorbent (ELISA) 10-15 These results demonstrate BCA offers 100-150-fold enhancement in over traditional colorimetric ELISA. Furthermore, detected infection 3...
We developed a europium nanoparticle-based immunoassay (ENIA) for the sensitive detection of anthrax protective antigen (PA). The ENIA exhibited linear dose-dependent pattern within range 0.01 to 100 ng/ml and was approximately 100-fold more than enzyme-linked immunosorbent assay (ELISA). False-positive results were not observed with serum samples from healthy adults, mouse plasma without PA, or collected mice injected lethal factor edema alone. For spiked sensitivities ELISA 100% (11/11...
Abstract During the past norovirus (NoV) epidemic season, a new GII.17 variant emerged as predominant NoV strain, surpassed GII.4 NoVs, causing outbreaks of acute gastroenteritis (AGE) in China. Here we report study an AGE outbreak elementary school December 2014 caused by to explore potential mechanism behind sudden epidemics NoV. A total 276 individuals were sick with typical infection symptoms vomiting (93.4%), abdominal pain (90.4%), nausea (60.0%) and diarrhea (10.4%) at attack rate...
It is urgently needed to update the comprehensive analysis about efficacy or effectiveness of COVID-19 vaccines especially during pandemic caused by SARS-CoV-2 Delta and Omicron variants. In general, current showed a cumulative 66.4%, 79.7%, 93.6% prevent infection, symptomatic COVID-19, severe respectively, but could not asymptomatic infection SARS-CoV-2. Furthermore, effectively variant although incidence breakthrough increased when intervals post full vaccination extended, suggesting...
Nanotechnology-based techniques are being widely evaluated in medical testing and could provide a new generation of diagnostic assays due to their high degrees sensitivity, specificity, multiplexing capabilities, ability operate without enzymes. In this article, we have modified nanoparticle-based biobarcode amplification (BCA) assay for early sensitive detection HIV-1 capsid (p24) antigen by using antip24 antibody-coated microplates capture viral streptavidin-coated DNAs signal...
The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it detectable several days earlier than host-generated antibodies (which are the target almost all current tests used in field) and can be to design very sensitive assays without need for PCR. Here, we present an ultrasensitive capacitive immunosensor that capable detecting subattogram per milliliter concentrations which our knowledge lowest level detection ever reported. Dilution...
We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented screening of variants in resource-limited settings. This approach can adapted emerging mutations laboratories already conducting nucleic acid amplification tests using existing resources extracted acid.
HIV-1 CRF07_BC is one of the most common circulating recombinant forms (CRFs) in China. The question why and how expanded so rapidly remains unknown.
Abstract Rotaviruses are known to recognize human histo-blood group antigens (HBGAs) as a host ligand that is believed play an important role in rotavirus susceptibility and range. In this study, paired fecal saliva samples collected from children with viral gastroenteritis, well serum the general population south China were studied evaluate potential association between infections HBGA phenotypes. Rotavirus was detected 75 (28%) of 266 P[8] rotaviruses found be predominant genotype. The...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been continuously mutating since its first emergence in early 2020. These alterations have led this virus to gain significant difference infectivity, pathogenicity, and host immune evasion. We previously found that the open-reading frame 8 (ORF8) of SARS-CoV-2 can inhibit interferon production by decreasing nuclear translocation regulatory factor 3 (IRF3). Since several mutations ORF8 observed, therefore, present study, we...
Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating effectiveness of vaccine and stage recovery COVID-19 disease. A rapid accurate method detection SARS-CoV-2-specific still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform IgM IgG by aggregation-induced emission carbon dots conjugated with SARS-CoV-2 spike protein (SSP). The (AIE-CDs) are one best...
The 5′ untranslated region (5′UTR) of hepatitis C virus (HCV) contains an internal ribosome entry site (IRES) which directs translation the viral open reading frame (ORF). 5′UTR is highly conserved between isolates in both primary sequence and predicted secondary structure. We cloned sequenced regions (nt 18 5′UTRto nt 15 core coding sequence) HCV representing six major genotypes subcloned these into a bicistronic, dual luciferase reporter construct. relative expression two luciferases, one...
For more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe rapid simple gold nanoparticle (NP)-based genomic assay specific identification avian influenza virus H5N1 its discrimination from other major A strains (H1N1, H3N2). Capture intermediate oligonucleotides were designed based on consensus sequences matrix (M) gene H1N1, H3N2 viruses, hemaglutinin (HA) neuraminidase (NA) genes...
Chlamydia trachomatis and human papillomavirus (HPV) are the most common pathogens of sexually transmitted infections (STIs), which can increase risk cervical cancer infertility. The purpose this study was to evaluate prevalence, genotype factors C. and/or HPV infection in women attending annual physical examination, assistant reproductive treatment visiting gynecology clinics from Southern Hunan province China. Cervical-swab samples were collected 5006 participants. We found that overall...