A5052400597- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Influenza Virus Research Studies
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Animal Disease Management and Epidemiology
- Telomeres, Telomerase, and Senescence
- Biosensors and Analytical Detection
- Plant Virus Research Studies
- Hepatitis B Virus Studies
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- HIV/AIDS Research and Interventions
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- Hepatitis C virus research
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
United States Food and Drug Administration
2007-2025
Center for Biologics Evaluation and Research
2012-2024
Yale University
2014
Office of Extramural Research
2014
New York University
2003-2010
Johns Hopkins University
2010
National Institutes of Health
2009-2010
National Cancer Institute
2010
American Red Cross
2010
University of Turku
2009-2010
The assays used to detect XMRV in patients with chronic fatigue syndrome are unreliable.
We developed a europium nanoparticle-based immunoassay (ENIA) for the sensitive detection of anthrax protective antigen (PA). The ENIA exhibited linear dose-dependent pattern within range 0.01 to 100 ng/ml and was approximately 100-fold more than enzyme-linked immunosorbent assay (ELISA). False-positive results were not observed with serum samples from healthy adults, mouse plasma without PA, or collected mice injected lethal factor edema alone. For spiked sensitivities ELISA 100% (11/11...
Nanotechnology-based techniques are being widely evaluated in medical testing and could provide a new generation of diagnostic assays due to their high degrees sensitivity, specificity, multiplexing capabilities, ability operate without enzymes. In this article, we have modified nanoparticle-based biobarcode amplification (BCA) assay for early sensitive detection HIV-1 capsid (p24) antigen by using antip24 antibody-coated microplates capture viral streptavidin-coated DNAs signal...
For more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe rapid simple gold nanoparticle (NP)-based genomic assay specific identification avian influenza virus H5N1 its discrimination from other major A strains (H1N1, H3N2). Capture intermediate oligonucleotides were designed based on consensus sequences matrix (M) gene H1N1, H3N2 viruses, hemaglutinin (HA) neuraminidase (NA) genes...
The HIV epidemic in Cameroon is characterized by a high degree of viral genetic diversity with circulating recombinant forms (CRFs) being predominant. goal our study was to determine recent trends virus evolution and emergence drug resistance blood donors positive patients.Blood specimens 73 individuals were collected from three cities few villages viruses isolated co-cultivation PBMCs. Nested PCR performed for gag p17 (670 bp) pol (840 Env gp41 (461 genes. Sequences phylogenetically...
We report the development of a novel europium nanoparticle-based immunoassay (ENIA) for rapid detection influenza A and B viruses. The ENIA demonstrated sensitivities 90.7% (147/162) viruses 81.80% (9/11) compared to those an in-house reverse transcription (RT)-PCR assay in testing influenza-positive clinical samples.
Conventional methods for detection and discrimination of influenza viruses are time consuming labor intensive. We developed a diagnostic platform simultaneous identification characterization that uses combination nanomicroarray screening multiplex next-generation sequencing (NGS) assays laboratory confirmation. The was to target hemagglutinin, neuraminidase, matrix genes identify A B viruses. PCR amplicons synthesized by using an adapted universal primer all 8 gene segments 9 subtypes were...
Reassortment of 2009 (H1N1) pandemic influenza virus (pdH1N1) with other strains may produce more virulent and pathogenic forms, detection their rapid characterization is critical. In this study, we reported a "one-size-fits-all" approach using next-generation sequencing (NGS) platform to extensively identify viral genomes for diagnosis determination novel virulence drug resistance markers. A de novo module bioinformatics tools were used generate contiguous sequence types/subtypes. Of 162...
ABSTRACT The global impact of COVID‐19, caused by SARS‐CoV‐2, has infected millions, including those with HIV‐1. However, it is unclear if SARS‐CoV‐2 affects HIV‐1 reactivation from latency. Here, we used the U1 cell line to explore how infection latency, employing real‐time PCR assays and Western blot analysis. Our results show higher levels RNA after infection. Importantly, noticed enhanced replication in cells viruses carrying a deletion amino acids R 682 , 683 A 684 (RRAΔ) spike (S)...
Expression of the human telomerase RNA component gene, hTERC is essential for activity. The gene expressed during embryogenesis and then downregulated normal development, leaving most adult somatic cells devoid expression. During oncogenesis, however, re-expressed consequently contributing to unrestricted proliferative capacity many cancers. Thus identification molecular basis regulation in its deregulation cancer immediate interest. We have previously cloned promoter this study identified...
An array of CRFs have been identified in Cameroon, the most notable being CRF02_AG. HIV-1 East Province Cameroon is particularly diverse: a recent study, we found high proportion unique recombinant forms (URFs). Herein describe analysis full-length sequences two these URFs, which, after preliminary gag, pol, and env fragments, appeared to be novel CRF. This strain, CRF36_cpx, contains fragments that can assigned CRF01_AE, CRF02_AG, subtype A G radiations. Forty percent genome classified as...
HIV-1 in Cameroon is genetically diverse, but predominated by the circulating recombinant form (CRF) 02_AG, which cocirculates among an array of other CRFs, unique forms (URFs), and all group M subtypes. In particular, our studies diversity East Province found a high proportion URFs second generation recombinants (SGRs), suggesting this region may be breading ground for new CRFs. Herein we present full-length sequence analysis one such CRF, composed primarily (66%) unique, distant lineages...
BACKGROUND: Since the identification of xenotropic murine leukemia virus–related virus (XMRV) in prostate cancer patients 2006 and chronic fatigue syndrome 2009, conflicting findings have been reported regarding its etiologic role human diseases prevalence general populations. In this study, we screened both plasma peripheral blood mononuclear cells (PBMNCs) collected Africa from donors immunodeficiency Type 1 (HIV‐1)‐infected individuals to gain evidence XMRV infection geographic region....
The proximal promoter of the telomerase RNA gene, hTR, contains four Sp1 sites and one CCAAT box. We have carried out a functional analysis role these sequence elements. Two downstream box mediated negative regulation, while other two were positive regulators with strongest effect by regulatory site closely flanking Basal transcriptional activity is maintained via even when all are mutated, suggesting nuclear factor‐Y (NF‐Y) fundamental regulator hTR function. Chromatin immunoprecipitation...