A5032481614- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant and Biological Electrophysiology Studies
- Advanced Fluorescence Microscopy Techniques
- European and International Law Studies
- Intellectual Property Rights and Media
- Law and Political Science
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Advanced Biosensing Techniques and Applications
- Digitalization, Law, and Regulation
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- GABA and Rice Research
- Aluminum toxicity and tolerance in plants and animals
- Force Microscopy Techniques and Applications
- Electrostatic Discharge in Electronics
- Growth Hormone and Insulin-like Growth Factors
- European Socioeconomic and Political Studies
- Privacy, Security, and Data Protection
- Merger and Competition Analysis
- Biotin and Related Studies
Heinrich Heine University Düsseldorf
2020-2023
University of California, Berkeley
2011-2022
Carnegie Department of Plant Biology
2017-2022
Carnegie Institution for Science
2017-2022
Marquette University
1965-1967
Although Mg(2+) is essential for a myriad of cellular processes, high levels in the environment, such as those found serpentine soils, become toxic to plants. In this study, we identified two calcineurin B-like (CBL) proteins, CBL2 and CBL3, key regulators plant growth under high-Mg conditions. The Arabidopsis mutant lacking both CBL3 displayed severe retardation presence excess Mg(2+), implying elevated toxicity these Unexpectedly, cbl2 cbl3 plants retained lower Mg content than wild-type...
DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes
Sensitivity, dynamic and detection range as well exclusion of expression instrumental artifacts are critical for the quantitation data obtained with fluorescent protein (FP)-based biosensors in vivo. Current designs are, general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform create dual-FP large ranges by employing single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests stable reference FP (large Stokes shift LSSmOrange)...
Glutamate has dual roles in metabolism and signaling; thus, signaling functions must be isolatable distinct from metabolic fluctuations, as seen low-glutamate domains at synapses. In plants, wounding triggers electrical calcium (Ca 2+ ) signaling, which involve homologs of mammalian glutamate receptors. The hydraulic dispersal squeeze-cell hypotheses implicate pressure a key component systemic signaling. Here, we identify the stretch-activated anion channel MSL10 necessary for proper...
Slow anion channels (SLAC/SLAH) are efflux previously shown to be critical for stomatal regulation. However, detailed analysis using the β-glucuronidase reporter gene showed that members of SLAC/SLAH family predominantly expressed in roots, addition guard cells, implicating distinct function(s) roots. Comprehensive mutant analyses all slac/slah mutants indicated slah3 plants a greater growth defect than wild-type when ammonium was supplied as sole nitrogen source. Ammonium toxicity mimicked...
Land plants have evolved a host of anatomical and molecular adaptations for terrestrial growth. Many these are believed to be elaborations features that were present in their algal-like progenitors. In the model plant Arabidopsis, 10 Calcineurin B-Like proteins (CBLs) function as calcium sensors modulate activity 26 CBL-Interacting Protein Kinases (CIPKs). The CBL-CIPK network coordinates environmental responses helps maintain proper ion balances, especially during abiotic stress. We...
Plants use electrical and chemical signals for systemic communication. Herbivory, instance, appears to trigger local apoplasmic glutamate accumulation, signals, calcium waves that travel report insect damage neighboring leaves initiate defense. To monitor extra- intracellular concentrations in plants, we generated Arabidopsis lines expressing genetically encoded fluorescent sensors. In contrast cytosolically localized sensors, extracellularly displayed variants inhibited plant growth proper...
Insulin fractionation of human and canine pancreata sera has been studied by immunoassay. Several procedures designed to separate bound free insulin have utilized. Immunoassay demonstrated pancreatic a very low solubility in saline. However, cation-bound fraction could not be either the pancreas or serum. These results suggest that insulin-like activity rat diaphragm adipose tissue assay does represent insulin.
Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors trigger nuclear calcium oscillations, which decoded by calcium/calmodulin-dependent protein kinase (CCaMK) its target transcription factor interacting of DMI3 (IPD3). Genes encoding CCaMK IPD3 have been lost in multiple non-mycorrhizal lineages yet retained among mosses. Here, we...
SUMMARY Plants use electrical and chemical signals for systemic communication. Herbivory, instance, appears to trigger local apoplasmic glutamate accumulation, calcium waves that travel report insect damage neighboring leaves initiate defense. To monitor extra- intracellular concentrations in plants, we generated Arabidopsis lines expressing genetically encoded fluorescent sensors. In contrast cytosolically localized sensors, extracellularly displayed variants inhibited plant growth proper...
Interactions between physical forces and membrane proteins underpin many forms of environmental sensation acclimation. Microbes survive osmotic stresses with the help mechanically gated ion channels osmolyte transporters. Plant mechanosensitive have been shown to function in defense signaling. Here, we engineered genetically encoded osmolality sensors (OzTracs) by fusing fluorescent protein spectral variants MscL from E. coli or MSL10 A. thaliana. When expressed yeast cells, OzTrac reported...