Titanium oxide nanotube layers by anodization have received considerable attention in biomedical application. Previous studies demonstrated increased osteoblast (bone-forming cell) adhesion and function on compared with unanodized counterparts. More recently, one study showed amorphous TiO(2) diameter determined cell fate. The anatase phase is known to be much more beneficial for bone growth than phase, so there increasing demand explore the response of layers. For this reason, we evaluated...

Biomimetic cell membrane coated nanoparticles (NPs) with desirable features have been extensively applied for various personalized biomedicine. However, there not relative explorations by employing the nanocomplexes small interfering RNA (siRNA) delivery. Herein, Fe3O4@PDA NPs good photothermal capability were efficient siRNA loading and delivery, which then mesenchymal stem cells (MSCs) to form a membrane. The data showed that MSCs Fe3O4@PDA–siRNA (Fe3O4@PDA–siRNA@MSCs) maintained...

Bone regeneration disorders are a significant problem in patients with type 2 diabetes mellitus. marrow stromal cells (BMSCs) recognized as ideal seed for tissue engineering because they can stimulate osteogenesis during bone regeneration. Therefore, the aim of this study was to investigate osteogenic potential BMSCs derived from diabetic rats and pathogenic characteristics dysfunctional that affect osteogenesis. were isolated normal high-fat diet+streptozotocin-induced rats. Cell metabolic...

Abstract The aims of this study were to fabricate a novel titanium/silicon carbide (Ti/SiC) metal matrix nanocomposite (MMNC) by friction stir processing (FSP) and investigate its microstructure mechanical properties. In addition, the adhesion, proliferation osteogenic differentiation rat bone marrow stromal cells (BMSCs) on surface investigated. MMNC was observed both scanning transmission electron microscopy. Mechanical properties characterized nanoindentation Vickers hardness testing....

Qiu J, Yu W‐Q, Zhang F‐Q, Smales RJ, Y‐L, Lu C‐H. Corrosion behaviour and surface analysis of a Co–Cr two Ni–Cr dental alloys before after simulated porcelain firing. Eur J Oral Sci 2011; 119: 93–101. © 2011 This study evaluated the corrosion properties commercial cobalt–chromium (Co–Cr) alloy nickel–chromium (Ni–Cr) [beryllium (Be)‐free Be‐containing] porcelain‐firing process. Before firing, microstructure, composition hardness, electrochemical properties, metal‐ion release as‐cast...

Human periodontal ligament (PDL) cells underwent apoptosis after mechanical stretch loading. However, the exact signalling pathway remains unknown. This study aimed to elucidate how apoptotic caspases functioned in cyclic stretch-induced human PDL cells.In present study, 20% was selected load for 6 or 24 h. The following parameters were analyzed: rates, protein levels of caspase-3, -7, -8 and -9 activities caspase-8 -9. Subsequently, influences caspase-9 inhibitors on rate level activated...

Microstructures, mechanical, and biological properties of a novel Ti-6V-4V/zinc surface nanocomposite prepared by friction stir processing Chenyuan Zhu,1,2,* Yuting Lv,3,4,* Chao Qian,1,2,* Zihao Ding,3,5 Ting Jiao,1,2 Xiaoyu Gu,1,2 Eryi Lu,6 Liqiang Wang,3 Fuqiang Zhang1,2 1Department Prosthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School Medicine, 2Shanghai Key Laboratory Stomatology, Research Institute National Clinical Center 3State Metal Matrix Composites,...

Background/Aims: Long noncoding RNAs (lncRNAs) are critical regulators in various diseases including human cancer and could function as competing endogenous (ceRNAs) to regulate microRNAs (miRNAs). Methods: Quantitative real-time PCR (qRT-PCR) was used analyze the expression of lnc-SNHG1 miR-302/372/373/520 pituitary tumor tissues cell lines. Cell proliferation investigated using MTT count assays. The mechanisms by which affects progression were Western blot assays, transwell migration...

Fast growth is one of the most desired traits for all food animals, which affects profitability animal production. The Pacific oyster, Crassostrea gigas, an important aquaculture shellfish around world with largest annual Growth oyster has been greatly improved by artificial selection breeding, but molecular mechanisms underlying remains poorly understood, limited integrative breeding fast-growth other superior traits. In this study, comparative transcriptome analyses between fast-growing...

Ratiometric imaging of tumor-related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non-specific activation in living cells remains challenging. Herein, orthogonally sequential concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and (STAR) TK1 inside with enhanced reliability accuracy. By virtue synthesized CuO/MnO

A new approach for the detection of Hg(2+) is reported based on color changes from which gold nanoparticles (Au NPs) are surrounded by a layer HgS quantum dots to form in situ Au@HgS core-shell nanostructures. The surface plasmon resonance (SPR) absorption core was changed due shell formed Au NPs, brings colour change aqueous solution. Therefore, can be recognized visualizing nanostructures, and detected quantitatively measurement UV-vis spectra. Some effects were investigated detail. This...

A simple, rapid and sensitive colorimetric assay method for detection of Co2+ through thioglycollic acid (TGA) functionalized hexadecyl trimethyl ammonium bromide (CTAB) modified Au NPs has been discovered in our work. TGA CTAB can be aggregated quickly the presence a cooperative metal–ligand interaction. Transmission electron microscope (TEM), energy dispersive X-ray spectroscopy (EDS), UV-vis spectra were used to characterize aggregation. The is monitored by response NPs, had limit 3.0 ×...

Type 1 diabetes mellitus (T1DM) is associated with a series of bone complications, which are still great challenge in the clinic. Bone marrow stromal cells (BMSCs) crucial to remodeling and attractive candidates for tissue engineering. Hence, we aimed investigate whether impaired functions BMSCs play role pathogenesis complications T1DM. were isolated from normal streptozotocin-induced diabetic rats, their proliferation osteogenic differentiation ability analyzed. Diabetic demonstrated...

Inflammasomes have been reported to be present in periodontal inflammatory tissue, but the exact role of inflammasomes reactions especially those related mechanical stimulations has not clarified. In this study, it was shown that cyclic stretch activated nucleotide-binding oligomerization domain-like receptor containing pyrin domain 1 and 3 (NLRP1 NLRP3) induced release IL-1β pyroptosis via a caspase-1-related mechanism human ligament cells (HPDLCs). This study firstly demonstrated...

Alzheimer's disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only alleviate symptoms without curing disease, which serious issue influences quality life patients their caregivers. In recent years, stem cell technology has provided new insights into diseases, including Parkinson's amyotrophic lateral sclerosis. Currently, main sources cells include...

Transmembrane proteins are involved in an array of stress responses, particularly thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification characterization the Transient Receptor Potential (TRP) genes Pacific oyster (Crassostrea gigas) investigated their expression profiles after heat to identify critical TRPs potentially associated with thermal regulation. A total 66 TRP were identified C. gigas, which showed significant gene expansion tandem...