A5088772630- RNA and protein synthesis mechanisms
- Medical Practices and Rehabilitation
- RNA modifications and cancer
- RNA Research and Splicing
- Bryophyte Studies and Records
- Botany and Plant Ecology Studies
- Mediterranean and Iberian flora and fauna
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- Cystic Fibrosis Research Advances
- Peptidase Inhibition and Analysis
- Evolution and Genetic Dynamics
- German Literature and Culture Studies
- Wheat and Barley Genetics and Pathology
- Antimicrobial Peptides and Activities
- Fungal Plant Pathogen Control
- Genomics and Rare Diseases
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Mycotoxins in Agriculture and Food
- Bioactive Compounds and Antitumor Agents
University of Bern
2020-2024
Universität Hamburg
2015-2021
University of Potsdam
2013
BOKU University
2003
The genetic code is degenerate; thus, protein evolution does not uniquely determine the coding sequence. One of puzzles in evolutionary genetics therefore to uncover driving forces that result specific codon choice. In many bacteria, first 5–10 codons protein‐coding genes are often less frequently used rest genome, an effect has been argued arise from selection for slowed early elongation reduce ribosome traffic jams. However, genome analysis across species demonstrated region shows reduced...
Synonymous single nucleotide polymorphisms (sSNPs) are considered neutral for protein function, as by definition they exchange only codons, not amino acids. We identified an sSNP that modifies the local translation speed of cystic fibrosis transmembrane conductance regulator (CFTR), leading to detrimental changes stability and function. This introduces a codon pairing low-abundance tRNA is particularly rare in human bronchial epithelia, but other tissues, suggesting tissue-specific effects...
Ribosome-enhanced translational miscoding of the genetic code causes protein dysfunction and loss cellular fitness. During evolution, open reading frame length increased, necessitating mechanisms for enhanced translation fidelity. Indeed, eukaryal ribosomes are more accurate than bacterial counterparts, despite their virtually identical, conserved active centers. evolution eukaryotic organisms ribosome expansions at rRNA level occurred, which potentially increases options regulation...
Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR), with approximately 90% of patients harboring at least one copy disease-associated variant F508del. We utilized a yeast phenomic system to identify genetic modifiers F508del-CFTR biogenesis, from which ribosomal protein L12 (RPL12/uL11) emerged as molecular target. In present study, we investigated mechanism(s) suppression RPL12 rescues F508del synthesis and activity. Using ribosome profiling,...
Significance The same mutation can have different effects among individuals, and one reason for this is the presence of additional mutations, a relationship known as epistasis. Epistatic are difficult to predict. We report rare example positive epistasis between silent (that changes nucleotide but not encoded amino acid) missense mutation(s) change acid cause cystic fibrosis disease). Individually, each has detrimental effect, they beneficial when present together. underlying molecular...
Abstract Fine-tuned regulation of protein biosynthesis is crucial for cellular fitness and became even more vital when organismal complexity increased during the course evolution. In order to cope with this augmented demand translation control, eukaryal ribosomes have gained extensions both at ribosomal rRNA levels. Here we analyze functional role ES27L, an expansion segment in large subunit Saccharomyces cerevisiae. Deletion b-arm segment, called ES27Lb, did not hamper growth optimal...
We recently showed that solid-phase peptide synthesis using racemic amino acids yields stereorandomized peptides comprising all possible diastereomers as homogeneous, single-mass products can be purified by HPLC and stereorandomization modulates activity, toxicity, stability of membrane-disruptive cyclic linear antimicrobial (AMPs) dendrimers. Here, we tested if might compatible with target binding the example proline-rich AMP oncocin, which inhibits bacterial ribosome. Stereorandomization...
Protein biosynthesis is essential for any organism, yet how this process regulated not fully understood at the molecular level. During evolution, ribosomal RNA expanded in specific regions, referred to as rRNA expansion segments (ES). First functional roles of these expansions have only recently been discovered. Here we address role ES7La located large subunit factor recruitment yeast ribosome and potential consequences translation. Truncation has minor effects on biogenesis, translation...