There is growing concern about the relevance of in vitro antimicrobial susceptibility tests when applied to isolates P. aeruginosa from cystic fibrosis (CF) patients. Existing methods rely on single or a few grown aerobically and planktonically. Predetermined cut-offs are used define whether bacteria sensitive resistant any given antibiotic1. However, during chronic lung infections CF, populations exist biofilms there evidence that environment largely microaerophilic2. The stark difference...

Synonymous single nucleotide polymorphisms (sSNPs) are considered neutral for protein function, as by definition they exchange only codons, not amino acids. We identified an sSNP that modifies the local translation speed of cystic fibrosis transmembrane conductance regulator (CFTR), leading to detrimental changes stability and function. This introduces a codon pairing low-abundance tRNA is particularly rare in human bronchial epithelia, but other tissues, suggesting tissue-specific effects...

Proteotoxins such as ricin, abrin, botulinum neurotoxins type A and B (BoNT/A, BoNT/B) staphylococcal enterotoxin (SEB) are regarded potential biological warfare agents which could be used for bioterrorism attacks on the food chain. In this study we a novel immunisation strategy to generate high-affinity monoclonal polyclonal antibodies against native BoNT/A, BoNT/B. The were along with SEB abrin establish highly sensitive magnetic fluorescent multiplex bead array excellent sensitivities...

Botulism is a severe neurological disease caused by the complex family of botulinum neurotoxins (BoNT). Based on different serotypes known today, classification serotype variants termed subtypes has been proposed according to sequence diversity and immunological properties. However, relevance BoNT currently not well understood. Here we describe isolation novel Clostridium strain from food-borne botulism outbreak near Chemnitz, Germany. Comparison its neurotoxin gene with published sequences...

Plant and microbial toxins such as ricin, staphylococcal enterotoxin B (SEB), the botulinum neurotoxins (BoNT) are considered potential biological warfare agents. Specific screening methods are, therefore, required that enable unambiguous sensitive identification of these biohazards, particularly for occurrence in complex sample matrixes. The present study describes a combination multiplex-immunoaffinity purification approach, followed by matrix-assisted laser desorption/ionization...

We present results for the production of a pair on-shell Z bosons via gluon fusion. This process occurs both through and decay Higgs boson, continuum where boson couples to loop massless quarks or massive quark. calculate interference two processes its contribution cross section up including order O(alpha_s^3). The two-loop contributions amplitude are all known analytically, except loops top mass m. latter is important invariant bosons, (as measured by their leptonic products, m_4l), in...

ABSTRACT Botulinum neurotoxins are produced by the anaerobic bacterium Clostridium botulinum and divided into seven distinct serotypes (A to G) known cause botulism in animals humans. In this study, a multiplexed quantitative real-time PCR assay for simultaneous detection of human pathogenic C. A, B, E, F was developed. Based on TaqMan chemistry, we used five individual primer-probe sets within one PCR, combining both minor groove binder- locked nucleic acid-containing probes. Each...

Botulinum neurotoxins (BoNTs) cause the disease botulism, which can be lethal if untreated. There are seven known serotypes of BoNT, A–G, defined by their response to antisera. Many distinguished into differing subtypes based on amino acid sequence, and many further differentiated toxin variants. Previous work in our laboratory described use a proteomics approach distinguish subtype BoNT/A1 from BoNT/A2 where BoNT identities were confirmed after searching data against database containing...

We investigate how precisely the CP nature of 125 GeV Higgs boson, parametrized by a scalar-pseudoscalar mixing angle, can be determined in Higgs-to-tau-pair decay with subsequent tau-lepton decays to charged prongs at Large Hadron Collider (LHC). combine two methods order define an observable which is sensitive this angle: use rho-decay plane method for tau-to-rho and impact parameter all other major tau decays. For estimating precision angle measured LHC (13 TeV) we take into account...

The most crucial step in data processing from high-throughput sequencing applications is the accurate and sensitive alignment of reads to reference genomes or transcriptomes. detection insertions deletions (indels) errors introduced by platform misreading modified nucleotides essential for quantitative RNA-based (RNA-Seq) datasets identification genetic variations modification patterns. We developed a new, fast algorithm nucleic acid sequence analysis, FANSe, with adjustable mismatch...

In the context of a potential bioterrorist attack sensitive and fast detection functionally active toxins such as ricin from complex matrices is necessary to be able start timely countermeasures. One functional methods currently available for endpoint cytotoxicity assay, which suffers number technical deficits.This work describes novel online assay Ricinus communis agglutinin, that based on real-time cell electronic sensing system impedance measurement. Characteristic growth parameters Vero...

We investigate how precisely the CP nature of 125 GeV Higgs-boson resonance $$h$$ can be unraveled at LHC in its decays to $$\tau $$ pairs, $$h \rightarrow \tau ^-\tau ^+$$ . use a method which allows one determine scalar–pseudoscalar Higgs mixing angle $$\phi _\tau this decay mode. This extracted from distribution signed angle, denoted by $$\varphi _\mathrm{CP}^{*}$$ , we analyze for major charged-prong decays. For definiteness, consider production gluon fusion NLO QCD. take into account...

Background: Despite clinical interventional studies on the influence of diet periodontal inflammatory parameters, there has been no practice-based cross-sectional study from a German population to date that conducted both comprehensive dental and examination thorough validated assessment dietary behavior. Therefore, aim this pilot was evaluate, in proof concept, whether is correlation between overall surface area (PISA), parameters (pocket probing depths (PPD), attachment loss (CAL),...

Infant botulism is an acute life-threatening condition and diagnosis frequently delayed. Therefore, the best time window to administer specific antibodies, at present only etiology-based therapy, often missed, entailing long periods of hospitalization in PICU. Here we a 3-month-old boy with infant respiratory failure, who quickly favorably responded thiamine supplementation. From feces isolated Clostridium botulinum serotype A2. In addition producing neurotoxin A, this strain carried...

Background Radiation exposure remains an unceasing concern in angiographic procedures. Modern angiography machines such as analog image intensifiers (AII) or the new flat panel detectors (FPD) aim at a further dose reduction. Purpose To present area products (DAP) broad spectrum of therapeutic procedures, comparing AII to FPD system. Material and Methods A total 999 peripheral procedures performed with ( n = 562) system 437) were evaluated. DAP, fluoroscopy time, patients' body mass index...

Approximately 60% of transplanted islets undergo apoptosis within the first week post-transplantation into liver attributed to poor engraftment, immune rejection and toxicity immunosuppressive drugs. Understanding how extracellular matrix (ECM) components, drugs proinflammatory cytokines affect insulin secretion will contribute an improved clinical outcome islet transplantations. In this study, functional activity isolated murine was measured by glucose-stimulated (GSIS) electrophysiological...

There is growing concern about the relevance of in vitro antimicrobial susceptibility tests when applied to isolates P. aeruginosa from cystic fibrosis (CF) patients. Existing methods rely on single or a few grown aerobically and planktonically. Predetermined cut-offs are used define whether bacteria sensitive resistant any given antibiotic1. However, during chronic lung infections CF, populations exist biofilms there evidence that environment largely microaerophilic2. The stark difference...

This protocol describes the microarray-based quantification of human tRNAs. The tRNA microarray consists 40 full-length tDNA probes recognising all 54 nuclearly encoded isoacceptor tRNAs1. array has been shown to reliably distinguish isoacceptors with a sequence difference more than 8 nucleotides. Deacetylated tRNAs are selectively labeled fluorescent oligonucleotide hairpin which is covalently attached single-stranded 3' NCCA overhang, commen feature tRNAs2. For comparison, differently from...

The measurement of possible Higgs sector CP-violation in the tau decay channels at LHC is investigated. A CP-violating effect would manifest itself these modes characteristic spin-spin correlations lepton pairs which can be accessed using momenta and impact parameters charged particles. We examine a CP-sensitive observable for 125 GeV boson resonance gluon fusion channel LHC. Furthermore, we consider distribution this irreducible Drell-Yan background. By splitting events into two categories...

Abstract Few lethal pathogens in wild‐living primates have been described, and little is known about infectious diseases of the reproductive tract their possible impact on health reproduction. This report describes pathology isolation an alpha‐toxin producing strain Clostridium septicum a case necrotizing endometritis wild sooty mangabey found dead tropical rainforest West Africa.