A5057157008- CAR-T cell therapy research
- Plant Molecular Biology Research
- Multiple Myeloma Research and Treatments
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Cancer-related gene regulation
- Nutrition and Health in Aging
- T-cell and B-cell Immunology
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- Horticultural and Viticultural Research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Protist diversity and phylogeny
- Multiple Sclerosis Research Studies
- Protein Degradation and Inhibitors
- Monoclonal and Polyclonal Antibodies Research
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Plant and Fungal Interactions Research
Moffitt Cancer Center
2022-2025
University of Florida
2019-2021
During infection, Citrus tristeza virus (CTV) produces a non-coding subgenomic RNA referred to as low-molecular-weight 1 (LMT1), which for long time has been considered by-product of the complex CTV replication machinery. In this study, we investigated role LMT1 in infection cycle using variant that does not produce (CTV-LMT1d). We showed lack did halt ability replicate or form proper virions. However, mutant demonstrated significantly reduced invasiveness and systemic spread Nicotiana...
Plants grown in spaceflight experience novel environmental signals, including those associated with microgravity and ionizing radiation. Spaceflight triggers a response involving transcriptional re-programming altered cell morphology, though many aspects of this remain uncharacterized. We analyzed the spaceflight-induced transcriptome focus on genes that undergo alternative splicing to examine differential spaceflight-an unstudied characteristic molecular exposure. RNA sequence data obtained...
Abstract Purpose: Sarcopenia is a hallmark of cancer cachexia. Chimeric antigen receptor (CAR) T-cell therapy associated with an inflammatory state that may exacerbate sarcopenia. The relationship between CAR therapy, sarcopenia, and metabolism poorly understood. Experimental Design: In 83 large B-cell lymphoma patients, the skeletal muscle index (SMI) was measured from clinical images obtained at baseline days 30 90 post-therapy. Serum metabolomics (n=57 patients) performed in first 4...
Despite the growing resources and tools for high-throughput characterization analysis of genomic information, discovery genetic elements that regulate complex traits remains a challenge. Systems genetics is an emerging field aims to understand flow biological information underlies from genotype phenotype. In this study, we used systems approach identify evaluate regulators lignin biosynthesis pathway in Populus deltoides by combining genome, transcriptome, phenotype data population 268...
High-throughput transcriptomic and proteomic analyses are now routinely applied to study cancer biology. However, complex omics integration remains challenging often time-consuming. Here, we developed DRPPM-EASY, an R Shiny framework for integrative multi-omics analysis. We our application analyze RNA-seq data generated from a USP7 knockdown in T-cell acute lymphoblastic leukemia (T-ALL) cell line, which identified upregulated expression of TAL1-associated proliferative signature lines....
Alternative splicing (AS) is a mechanism of regulation the proteome via enabling production multiple mRNAs from single gene. To date, dynamics AS and its effects on protein sequences individuals in large genetically unrelated population trees have not been investigated. Here we describe diversity events within previously genotyped 268 Populus deltoides their putative downstream functional effects. Using robust bioinformatics pipeline, resulting transcript isoforms were discovered quantified...
<title>Abstract</title> Chimeric antigen receptor T-cells targeting BCMA have revolutionized the treatment of relapsed/refractory multiple myeloma (RRMM) with two approved products, idecabtagene vicleucel (ide-cel) and ciltacabtagene autoleucel (cilta-cel). To explore biological differences, we analyzed pre-infusion products (IP) CAR-enriched peripheral blood mononuclear cells (PBMCs) at expansion using single-cell RNA sequencing (scRNAseq) from 52 samples. Post-quality control 247,500...
ABSTRACT Crassulacean acid metabolism (CAM) is a specialized type of photosynthesis: stomata close during the day, enhancing water conservation, and open at night, allowing CO 2 uptake. Mesembryanthemum crystallinum (common ice plant) facultative CAM species that can shift from C 3 photosynthesis to under salt or drought stresses. However, molecular mechanisms underlying stress induced transition remain unknown. Here we determined time in M. stress. In parallel, single-cell-type...