A5031582777- Renal cell carcinoma treatment
- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- Ferroptosis and cancer prognosis
- Multiple and Secondary Primary Cancers
- Renal Diseases and Glomerulopathies
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Renal and related cancers
- CNS Lymphoma Diagnosis and Treatment
- Prostate Cancer Diagnosis and Treatment
- Urologic and reproductive health conditions
- Immune cells in cancer
- Genital Health and Disease
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cardiac, Anesthesia and Surgical Outcomes
- Urinary and Genital Oncology Studies
- Prostate Cancer Treatment and Research
- Urological Disorders and Treatments
- Health Systems, Economic Evaluations, Quality of Life
- Diversity and Career in Medicine
- Renal and Vascular Pathologies
- Urinary Bladder and Prostate Research
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
Oregon Health & Science University
2018-2025
OHSU Knight Cancer Institute
2023-2025
Portland VA Medical Center
2023-2025
VA Portland Health Care System
2024
Providence Portland Medical Center
2024
Moffitt Cancer Center
2020-2023
Lexington Medical Center
2023
Oregon Health and Science University Hospital
2023
GTx (United States)
2021
Oregon Medical Research Center
2018-2020
<h3>Importance</h3> Clinical trials have shown an overall survival (OS) benefit associated with first-line immunotherapy (IT) and combination targeted therapy (TT) IT regimens compared TT among patients metastatic clear cell renal carcinoma (RCC). Generalizability of these findings in a real-world cohort outside clinical trial setting is unclear. <h3>Objective</h3> To assess the association TT, IT, OS RCC. <h3>Design, Setting, Participants</h3> This retrospective propensity-matched study...
Level I evidence has failed to demonstrate an overall survival (OS) advantage for cytoreductive nephrectomy in patients with metastatic clear cell renal carcinoma (ccRCC) the modern era, which is at odds observational studies reporting a marked OS benefit associated these operations. These were not designed adjust unmeasured confounding.To assess whether improved ccRCC.This cohort study identified ccRCC National Cancer Database from January 1, 2006, December 31, 2016, who received systemic...
Abstract It is poorly understood how the tumor immune microenvironment influences disease recurrence in localized clear-cell renal cell carcinoma (ccRCC). Here we performed whole-transcriptomic profiling of 236 tumors from patients assigned to placebo-only arm a randomized, adjuvant clinical trial for high-risk ccRCC. Unbiased pathway analysis identified myeloid-derived IL6 as key mediator. Furthermore, novel myeloid gene signature strongly correlated with and overall survival on uni-...
Introduction In clear cell renal carcinoma (ccRCC), tumor-associated macrophage (TAM) induction of CD8+T cells into a terminally exhausted state has been implicated as major mechanism immunotherapy resistance, but deeper biological understanding is necessary. Methods Primary ccRCC tumor samples were obtained from 97 patients between 2004 and 2018. Multiplex immunofluorescence using lymphoid myeloid markers was performed in seven regions interest per patient across three predefined zones,...
A clearer understanding of the tumor immune microenvironment (TIME) in metastatic clear cell renal carcinoma (ccRCC) may help to inform precision treatment strategies. We sought identify clinically meaningful TIME signatures ccRCC. studied tumors from 39 patients with ccRCC using quantitative multiplexed immunofluorescence and relevant marker panels. Cell densities were analyzed three regions interest (ROIs): core, tumor-stroma interface stroma. Patients stratified into low- high-marker...
No AccessJournal of UrologyAdult Urology1 Oct 2021Pathological Downstaging and Survival Outcomes Associated with Neoadjuvant Chemotherapy for Variant Histology Muscle Invasive Bladder CancerThis article is commented on by the following:Editorial Comment Nicholas H. Chakiryan, Da David Jiang, Kyle A. Gillis, Elizabeth Green, Ali Hajiran, Lee Hugar, Logan Zemp, Jingsong Zhang, Rohit Jain, Jad Chahoud, Michael Poch, Brandon J. Manley, Roger Li, Wade Sexton, Scott M. Gilbert ChakiryanNicholas...
Abstract Immune-modulating systemic therapies are often used to treat advanced cancer such as metastatic clear cell renal carcinoma (ccRCC). Used alone, sequence-based biomarkers neither accurately capture patient dynamics nor the tumor immune microenvironment. To better understand ecology of this microenvironment, we quantified infiltration across three distinct ccRCC cohorts using complementarity determining region-3 (CDR3) sequence recovery counts in tumor-infiltrating lymphocytes and a...
Abstract Summary Multiplex immunofluorescence (mIF) staining combined with quantitative digital image analysis is a novel and increasingly used technique that allows for the characterization of tumor immune microenvironment (TIME). Generally, mIF data to examine abundance cells in TIME; however, this does not capture spatial patterns throughout TIME, metric recognized as important prognosis. To address gap, we developed an R package spatialTIME enables data, well iTIME web application...
Abstract Background Immunotherapy has improved survival for patients with advanced clear cell renal carcinoma (ccRCC), but resistance to therapy develops in most patients. We use cellular-resolution spatial transcriptomics immunotherapy naïve and exposed primary ccRCC tumors better understand resistance. Results Spatial molecular imaging of tumor adjacent stroma samples from 21 suggests that viable following harbor more stromal CD8 + T cells neutrophils than tumors. YES1 is significantly...
While vascular endothelial growth factor tyrosine kinase inhibitors (VEGF-TKIs) are a mainstay of treatment for advanced renal cell carcinoma (RCC), mechanisms resistance to VEGF-TKIs remain under ongoing investigation. To assess transcriptomic changes in clear-cell RCC (ccRCC) and non-ccRCC exposed VEGF-TKI, we analyzed differential single-cell gene expression tumor-organoids cabozantinib versus control solvent. In ccRCC organoid cells, LRRC75A was notably highly associated with exposure...
While vascular endothelial growth factor tyrosine kinase inhibitors (VEGF-TKIs) are a mainstay of treatment for advanced renal cell carcinoma (RCC), mechanisms resistance to VEGF-TKIs remain under ongoing investigation. To assess transcriptomic changes in clear-cell RCC (ccRCC) and non-ccRCC exposed VEGF-TKI, we analyzed differential single-cell gene expression tumor-organoids cabozantinib versus control solvent. In ccRCC organoid cells, LRRC75A was notably highly associated with exposure...
Immune infiltration is typically quantified using cellular density, not accounting for clustering. Tumor-associated macrophages (TAM) activate oncogenic signaling through paracrine interactions with tumor cells, which may be better reflected by local clustering than global density metrics. Using multiplex immunohistochemistry and digital pathologic analysis we myeloid cell markers in 129 regions of interest from 55 samples 35 patients metastatic ccRCC. CD68+ cells were found to clustered...
Cancer-associated fibroblasts (CAF) are highly prevalent cells in the tumor microenvironment clear cell renal carcinoma (ccRCC). CAFs exhibit a pro-tumor effect vitro and have been implicated proliferation, metastasis, treatment resistance. Our objective is to analyze geospatial distribution of with proliferating apoptotic ccRCC determine associations survival systemic treatment. Pre-treatment primary samples were collected from 96 patients metastatic ccRCC. Three adjacent slices obtained 2...
Upper tract urothelial carcinoma with clinically positive regional lymph nodes is an aggressive disease state a high propensity for metastasis and death. The current literature limited regarding national practice patterns outcomes in this patient population.We identified 1,658 patients the NCDB (National Cancer Database) who had cN+M0 upper carcinoma. Patients were stratified into treatment groups. We compared baseline tumor characteristics between groups, completed survival analysis using...
No AccessJournal of UrologyAdult Urology1 Oct 2021Patterns Recurrence following Inguinal Lymph Node Dissection for Penile Cancer: Optimizing Surveillance StrategiesThis article is commented on by the following:Editorial Comment Nicholas H. Chakiryan, Aaron Dahmen, Marco Bandini, Filippo Pederzoli, Laura Marandino, Maarten Albersen, Eduard Roussel, Yao Zhu, Ding-Wei Ye, Antonio A. Ornellas, Mario Catanzaro, Oliver W. Hakenberg, Axel Heidenreich, Friederike Haidl, Nick Watkin, Michael Ager,...
Physician work relative value units are determined based on operative time, technical skill, mental effort and stress. In theory, should account for the time involved in a procedure, resulting similar per unit short long procedures. We assessed whether is adequately accounted by current assignments.The American College of Surgeons National Surgical Quality Improvement Program database was reviewed from 2015 to 2017. The 50 most frequently coded urology CPT codes were included study. primary...